Citations

Reagents:

LPS was obtained from List Biological Laboratories (San Jose, CA, USA). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

RNAi experiments:

RNAi using RAW264.7 cells was performed largely as described [43]. … Twenty-four hours after siRNA transfection, cells were stimulated with the indicated PAMPs for six hours. Doses used were 20 ng/ml LPS (List Biological Labs), …

Author indicated that the following LPS product was used:

• Product #201 – LPS from Escherichia coli O111:B4

Reagents:

… LPS from E. coli O111:B4 for in vitro experiments was purchased from List Biological Laboratories; …

Immunoprecipitations and Western blotting analysis:

HEK 293T cells were transfected with plasmids encoding 4-1BBL and TLR4, and cell lysates were prepared and incubated with the appropriate primary antibodies before being incubated with Protein G-agarose. The immunoprecipitates were then resolved by SDS-PAGE and analyzed by Western blotting according to standard protocols. To examine physical interactions between endogenous proteins, we treated mouse peritoneal macrophages with LPS, subjected cell lysates to immunoprecipitation with anti-TLR4 or anti-TIRAP antibodies, and then analyzed samples by Western blotting, which was performed with chemiluminescence substrate, and bands corresponding to proteins of interest were visualized with x-ray film or with the Odyssey scanner (LI-COR Bioscience, Lincoln, NE).

• Product #201 – LPS from Escherichia coli O111:B4

EAE:

…Immediately following the MOG 33-55 injection, mice were injected ip with 300 µl of salt-free pertussis toxin (List Biological Laboratories, INC, Campbell, CA at 1 ng/l) on the same day and again 48 h later.

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

EAE:

Active EAE was induced using previously-described protocols [79]. In accordance with Canadian Council on Animal Care (CCAC) and UBC guidelines, female 6-8 week old CL57BL/6 mice … 200 ng Pertussis toxin (List Biologicals, Campbell, CA) was delivered intraperitoneally at immunization and two days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Control animals were administered CFA without PLP peptide. Two and 48 h after EAE induction, all animals received an intraperitoneal injection of 400 ng of pertussis toxin (List Biological Laboratories, Campbell, Calif., USA) in 100 l of saline. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

S1P Receptor Agonists and Other Reagents:

… Pertussis toxin was purchased from List Biological Laboratories (Campbell, CA). …

In Vitro Assessment of Downstream Signaling Pathways Mediated by S1P1 Agonists Activation of G i -coupled GPCR S1P 1 triggers multiple cell signaling pathways:

… Receptor-specific signaling was demonstrated by the absence of signal on parental cells and receptor-expressing cells in the presence of pertussis toxin (PTX) for Ca2+ mobilization (Suppl. Fig. S1A), cAMP (Suppl. Fig. S1B), and pERK (Suppl. Fig. S1C) assays. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Determination of loss of mitochondrial membrane potential (m):

… followed by pretreatment with 50 ng/ml PTX (List Biological Laboratories, Campbell, CA, USA) for 24 h. After washout of PTX, the cell density of PTX-treated and control group cells (non-treated cells) were again adjusted to 1105 cells/ml, followed by treatment with 50 M Pg for an additional 24-h incubation. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction:

… Pertussis toxin (200 ng/100 µl/mouse, List Biological Laboratories, Campbell, CA, USA) was injected intraperitoneally to these mice on the day of immunization (day 0) and again 48 h later (day 2). EAE was scored daily as described …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE Using the Myelin Oligodendrocyte Glycoprotein_35-55 Peptide:

…While under anesthesia, the mice were also injected with 200 ng of pertussis toxin (2 ng/µl; List Biologicals Laboratories, Campbell, CA, http://www.listlabstage.wpengine.com) through intraperitoneal (ip) ad- ministration. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)