Citations

IMC isolation and differentiation:

B6 mice were immunized with an emulsion of 100 ml PBS and 100 ml CFA… Each animal also received 200 ng pertussis toxin (PT; List Biological Laboratories) i.v. on days 0 and 2 postimmunization. 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental protocol:

…To examine the role of chemoattractant receptors and CCR4 on Treg migration during CS, mice underwent sensitization and challenge as already described. Subsequently, anesthetized mice were injected intradermally with either PTx (List Biological Laboratories, Campbell, CA; 100 ng in PBS)…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE exacerbation, pharmacokinetics and treatment:

C57BL/6 mice were immunized subcutaneously at four sites in the flanks with 100 µg hMOG3555 peptide… 200 ng of pertussis toxin (List Biological Laboratories) was injected i.p. on days 0 and 2 to disrupt the blood-brain barrier. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell Line and Cell Culture:

… After mice were killed, the eyeballs were enucleated by the pathogen-free disposable punch (2.5 mm), and washed with Ca²⁺-free PBS (pH 7.2) containing 1X antibiotic-antimycotic… After 7 to 10 days, nearly 100% confluence of the cells formed, the half corneal button could be placed in a new cell culture plate to start a new explant culture as described above. …and subcultured at CnT-50 containing 100 ng/mL cholera toxin (LIST Biological Laboratories, Inc., Campbell, CA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Cell culture and treatments:

… 10 ng/mL cholera toxin (List Biological Laboratories, Inc.), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… LPS was obtained from List Biological Laboratories (San Jose, CA, USA) and was dissolved in endotoxin-free water. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… Reagents and cell culture:

Staphylococcal enterotoxin B was obtained from List Biological Laboratories Inc. (Campbell, CA, USA). …

Reagents:

LPS (Escherichia coli serotype O111:B4) was purchased from List Biological Laboratories, INC (Campbell, CA); …

• Product #201 – LPS from Escherichia coli O111:B4

Induction, treatment, and clinical evaluation of EAE:

The murine EAE model was produced as described previously [56]. Briefly, female C57BL/6 mice were immunized on day 0 by subcutaneous (s.c.) injection with 150 µl of an emulsion containning MOG₃₅₅₅ CFA …Each mouse received an additional 400 ng of Bordetella pertussis toxin (List Biological Laboratory) by intraperitoneal (i.p.) injection in 200 µl of PBS on day 0 and day 2 post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Anatomy:

A separate set of tissue containing NTS coming from the same group of treated rats (n = 4) was incubated overnight at room temperature with the primary antibody for CTb (1:20,000, Goat anti-CTb; 703, List Biological Laboratories)

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)