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4694 citations found

T cells become licensed in the lung to enter the central nervous system

Odoardi, F;Sie, C;Streyl, K;Ulaganathan, VK;Schlger, C;Lodygin, D;Heckelsmiller, K;Nietfeld, W;Ellwart, J;Klinkert, WE;Lottaz, C;Nosov, M;Brinkmann, V;Spang, R;Lehrach, H;Vingron, M;Wekerle, H;Flgel-Koch, C;Flgel, A;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Product: ULTRA PURE LPS from Escherichia coli O111:B4

  • Oligodeoxynucleotides and reagents:

    Ultra pure lipopolysaccharide (LPS; from Escherichia coli 0111:B4) was purchased from List Biological Laboratories, Inc. (Campbell, CA). …

    Transfection and reporter gene assays:

    RAW264.7 cells (2 106 cells/well) were plated into 6-well plates and then incubated for 24 hr to reach approximately 80% confluence. Cells were co-transfected with pRL-TK-luciferase (1 g) and wild type or mutant Irak-m promoter-luciferase reporter constructs (3 g) using Lipofectamine (Invitrogen) according to the manufacturer’s protocol. … Transfected cells were incubated for 6 hr, pooled, and washed 3 times with culture media. Cells (1 or 2105 cells/well in 96-well culture plates) were stimulated with medium, CpG DNA (6 g/ml), non-CpG DNA (6 g/ml), LPS (50 ng/ml), …

Product: LPS from Salmonella minnesota R595 (Re)

Cultured primary macrophage activation by lipopolysaccharide depends on adsorbed protein composition and substrate surface chemistry

Diekjiirgen, D;Astashkina, A;Grainger, DW;Holt, D;Brooks, AE;

Product: Unspecified List Labs LPS

Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

  • 2.2.2. Cell Culture

    HEK293 human embryonic kidney cells (gift of Frank Graham) were maintained
    in Minimum Essential Medium (MEM) supplemented with 10% fetal bovine serum
    (FBS). This cell line has been transformed with the E1 region of type 5
    adenovirus (Graham et al., 1977). NIH3T3 murine embryonic fibroblasts (ATCC
    #CRL-1658) were maintained in high glucose Dulbecco’s Modified Eagle Medium
    (DMEM) supplemented with 10% FBS. hTERT-HME1 human mammary
    epithelial cells (ATCC #CRL-4010) were maintained in DMEM/F12 supplemented
    with 10 mM HEPES, 10 µg/mL insulin, 5 mg/mL BSA, 10 ng/mL Cholera toxin, 10
    ng/mL epidermal growth factor (EGF), and 0.5 µg/mL hydrocortisone. MCF-10A
    human mammary epithelial cells (ATCC #CRL10317) were maintained in
    modified DMEM/F12 with 5% FBS, 10 µg/mL insulin, 10 ng/mL Cholera toxin, 20
    ng/mL EGF, and 0.5 µg/mL hydrocortisone. MTHJ murine mammary
    adenocarcinoma cells were established as described (Hummel, Safroneeva, and
    Mossman, 2005) and maintained in high glucose Dulbecco’s Modified Eagle
    Medium (DMEM) supplemented with 10% FBS. ZR-75-1 human breast cancer
    cells (ATCC #CRL-1500) were cultured in RPMI 1640 supplemented with 10%
    FBS. All cell lines were supplemented with 2 mM L-glutamine, 0.1 mg/mL
    penicillin, and 100 U/mL streptomycin, and grown at 37ºC in a humidified
    atmosphere at 5% CO2. All media were purchased from Sigma Chemicals and
    all supplements were purchased from Gibco except for cholera toxin (List
    Biologicals)
    .

    Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Galectin-1 deactivates classically activated microglia and protects from inflammation-induced neurodegeneration

Starossom, SC;Mascanfroni, ID;Imitola, J;Cao, L;Raddassi, K;Hernandez, SF;Bassil, R;Croci, DO;Cerliani, JP;Delacour, D;Wang, Y;Elyaman, W;Khoury, SJ;Rabinovich, GA;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Product: SNAPtide® Peptide Substrate (o-Abz/Dnp) for C. botulinum Type A Neurotoxin

  • SNAPtide assay of BoNT/A LC with screened compounds:

    SNAPtide (o-Abz-Dnp) was purchased from List Biological Laboratories and was used
    for analyzing the kinetics of protease inhibition.  The BoNT/A LC is included in a mixture
    (denoted Big Mix) to minimize discrepancies in concentration of enzyme and to have a constant
    concentration of BoNT/A LC throughout the whole experiment.  Big Mix includes 1xPBS,
    20M Zn2+, and 0.2M LC/A-SUMO.   Compounds B, D, and E were dissolved in DMSO and
    the LC Buffer (1xPBS, 20M Zn2+) and DMSO was tested as a negative control to determine any
    possible interference in fluorescence.  The catalytic activity of BoNT/A LC is largely insensitive
    to the presence of DMSO, making this an ideal co-solvent (Boldt et al, 2006).   The SNAPtide
    assay was initiated by the addition of 2M SNAPtide and the reaction was allowed to proceed to
    5% completion and the initial rate was determined.  The protease activity was monitored by the
    increase of donor fluorescence intensity.  The compound 7-(phenyl(8-quinolinylamino)-8
    quinolinol, better known as QAQ (84096), has previously shown in an HPLC-based enzymatic
    assay to inhibit BoNT/A LC by 74% at 20M enzyme concentration (Lai et al, 2009). …

Quetiapine, an atypical antipsychotic, is protective against autoimmune-mediated demyelination by inhibiting effector T cell proliferation

Mei, F;Guo, S;He, Y;Wang, L;Wang, H;Niu, J;Kong, J;Li, X;Wu, Y;Xiao, L;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

  • EAE Mice Model and Treatment Protocols:

    C57BL/6 mice were obtained from the Animal Center of Third Military Medical University. All experiments were performed in accordance with Health Guide for the Care and Use of Laboratory Animals, with the approval of Third Military Medical University Committee on Animal Care (permission NO: SCXK-JUN-2007-015). Female C57BL/6 mice (N=43, 8 weeks old) were immunized subcutaneously with 200 µg of MOG3555 peptide (Invitrogen, Carlsbad, CA) emulsified in complete Freund adjuvant (CFA, Sigma Aldrich, Saint Louris, MO) on Day 0 and Day 7, and received 300 ng pertussis toxin (PT, List Biological Laboratories, Campbell, CA) in 0.1 ml PBS intraperitoneal at the time of immunization and 48 hours later. …

    Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
    Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
    Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
    Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Protection from the acquisition of Staphylococcus aureus nasal carriage by cross-reactive antibody to a pneumococcal dehydrogenase

Lijek, RS;Luque, SL;Liu, Q;Parker, D;Bae, T;Weiser, JN;

Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

  • Immunization with Purified Antigens:

    As previously described (38), mice were immunized intranasally with 4 µg of recombinant protein and 1 µg cholera toxin as adjuvant (List Biological Laboratories) per 20 µL dose. Control mice received adjuvant alone. Three immunizations were given at weekly intervals, followed by intranasal S. aureus challenge at week 5, as described above.

    Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae