Bacterial Toxin Research Citations

EAE induction and ex vivo IL-17 production:

EAE was induced according to the previously described method (21). Briefly, C57BL/6 mice were immunized subcutaneously with 100 l of an emulsion containing 100 g/mouse of MOG 3555 in 50 l of PBS and 50 l of complete Freunds adjuvant (CFA) on day 0. CFA was prepared by mixing incomplete Freunds adjuvant (Difco, Detroit, MI) with 20mg/ml of Mycobacterium tuberculosis H37RA (Difco). One hundred nanograms per mouse of pertussis toxin (List Biological Laboratories Inc., Campbell, CA) was intravenously injected on days 0, 2 and 7. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin (PTX) was obtained from List Biological Laboratories (Campbell, CA), …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction, prevention, and treatment:

Mice (1216 wk old) were immunized s.c. with 50 mg MOG3555 peptide (MEVGWYRSPFSRVVHLYRNGK; AnaSpec) and 500 mg Mycobacterium tuberculosis (Difco) emulsied in incomplete Freunds adjuvant (Difco). In addition, the animals received 200 ng pertussis toxin (List Biological Laboratories) i.p. on days 0 and 2. The severity of EAE was monitored and graded on a clinical score of 05: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mouse immunizations:

Six- to eight-week-old female SJL/J mice were immunized with 300 mg AqpZ protein or a mixture containing equal ratios of homologous AqpZ peptides (AqpZ5567 [GHISGGHFNPAVT], AqpZ82101 [VGYVIAQVVGGIVAAALLYL], AqpZ174190, and AqpZ203220 [EQLWFFWVVPIVPGIIGG]) or homologous Aqp4 peptides (Aqp489101 [GHISGGHINPAVT], Aqp4116135 [VFYIAAQCLGAIIGAGILYL], AqpZ201217, and Aqp4228245 [ENHWIYWVGPIIGAVLAG]) (University of Illinois Proteomic Facility) and emulsied in CFA, as we reported previously (33, 36). Control mice were injected with CFA only. All of the mice also received 100 ng pertussis toxin (List Biological Laboratories, Campbell, CA) i.p. 24 h postimmunization (PI). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Mice received an intravenous injection of 200 ng pertussis toxin (List Biological Laboratories) in 100 l PBS on the same day and at 48 h after the injection of TNF- and IFN-. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell Lines and Chemicals:

… cholera toxin from List Biological Laboratories Inc. (CA, USA); …

Intestinal Fluid Secretion and Absorption Measurements in Mice:

… Loops were injected with 100 µl of phosphate-buffered saline (PBS) or PBS containing cholera toxin (1 µg/loop) with or without DQA (100 µM).

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Purified LPS was purchased from List Biological Laboratories (San Jose, CA, USA). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… immunized mice) (n = 10). These animals were inoculated intranasally twice with three weeks interval with 100 µg HS5 lysate + 5 µg cholera toxin (CT) (List Biological Laboratories Inc., Madison, NJ, USA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Macrophage Culture Conditions:

We harvested primary macrophages using four mice per strain, by intraperitoneal lavage 4 days after injection with thioglycollate (BD, Sparks, MD, USA). … The next day, cells were incubated for 4 hr with 1% FBS DMEM media in control-treated cells, media plus 2 ng/ml LPS (List Biological Inc., Campbell, CA, USA), or media plus 50 g/ml OxPAPC.

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… BMDMs were then replated in 6- or 24-well plates for experiments. BMDMs were primed with 0.5 mg ml21 Escherichia coli LPS serotype O1101:B4 (List Biological Laboratories) for 4 h and then stimulated with ATP (5 mM; Sigma), DOTAPtransfected flagellin (20 mg ml21 ; InvivoGen), or Lipofectamine 2000-transfected pcDNA3.1 (1 mg ml21 ; Invitrogen). …

• Product #201 – LPS from Escherichia coli O111:B4