Bacterial Toxin Research Citations

January 6, 2025

Vaccines

Choi, E;Pyo, S;Kim, S;Jeon, J;Rhie, G;Yun, M;Yi, H;Chung, Y;

Product: Botulinum Neurotoxin Type B Complex, Nicked, from Clostridium botulinum

2.4. Preparation of Toxins

BoNT/A was harvested from C. botulinum strain ATCC19397 following a 6-day anaerobic culture as described previously [18,19]. BoNT/B complex was purchased from List Bio Laboratory Inc. (Campbell, CA, USA). The potencies of the toxins were determined using standard mouse bioassays with established LD50 values for BoNT/A and B. The LD50 values of BoNT/A and B in mice were confirmed to be 0.1 ng and 0.18 ng, respectively.

December 31, 2024

Nutrients

Gentili, V;Schiuma, G;Dilliraj, L;Beltrami, S;Rizzo, S;Lara, D;Giovannini, P;Marti, M;Bortolotti, D;Trapella, C;Narducci, M;Rizzo, R;

Product: Anthrax Lethal Factor (LF), Recombinant from B. anthracis

2.13. Priming of Inflammasomes

Inflammasome activation was measured according to Primiano et al.’s description [28]. HCM3 cells were plated in EMEM without supplements at 1 × 105 cells/well. Cells were stimulated for 1 h with 10 g/mL (13.4 mM) of nigericin (tlrl-nig, InvivoGen, San Diego, CA, USA) for 2 h with 2 mM ATP (tlrl-atpl, InvivoGen), or overnight with 200 g/mL of monosodium urate crystals (MSU; tlrl-msu, InvivoGen) for NLRP3 induction; flagellin/DOTAP complex, obtained with 25 μL HBS buffer with 500 ng flagellin (final concentration at 1 μg/mL; tlrl-stfla, InvivoGen) and 3 μL DOTAP (11202375001, Sigma-Aldrich) for each well for NLRC4 induction; with 0.5 mL Opti-MEM supplemented with GlutaMax (Opti-MEM™ Reduced Serum Medium containing GlutaMAX™ Supplement, 51985034, Gibco, Grand Island, NY, USA) containing 1 μg/mL Bacillus anthracis lethal factor (172B, List Biological Laboratories, Campbell, CA, USA) for 3–6 h for NLRP1 induction; and with a poly(dA:dT)/lipofectamine complex, obtained by mixing 50 μL Opti-MEM containing 1 μg poly(dA:dT) (final concentration at 2 μg/mL) with 50 μL Opti-MEM containing 2.5 μL lipofectamine 2000 (11668027, Thermo Fisher’s Scientific, Waltham, MA, USA) for each well for AIM2 induction. …

• Product #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis

December 31, 2024

The Journal Of Infectious Diseases

Baliban, SM;Shridhar, S;Luo, K;Kolasny, J;Hyun, S;Zhao, Z;Tennant, SM;Cross, AS;

Product: LPS from Escherichia coli O111:B4

with 20 mg of D-galactosamine (DGalN, MilliporeSigma), to sensitize them to the biological effects of endotoxin, and simultaneously challenged with 5 ng of E. coli O111:B4 LOS (List Biological Laboratories, Campbell, CA) [23]. Survival was monitored daily up to 30 days. Animals that were overtly moribund were immediately euthanized and recorded as having died

December 30, 2024

Nature Communications

Wu, Y;Park, J;Le, Q;Byun, J;Choi, J;Xu, H;Lee, J;Oh, Y;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

unds adjuvant (CFA) (Chondrex, Woodinville, WA, USA, cat# 7001), consisting of 4?mg/mL of heat-killed _M. Tuberculosis_ H37 RA. Subsequently, the mice received 400?ng of Pertussis toxin (PTX) (List Biological Labs, Campbell, CA, USA, cat# 180) intraperitoneally at 2 and 24?h post-injection of the emulsion. Intravenous injection of Cy5-labeled nanoparticles at a dose of 40?mg per kg was ... unds adjuvant (CFA) (Chondrex, Woodinville, WA, USA, cat# 7001), consisting of 4?mg/mL of heat-killed _M. Tuberculosis_ H37 RA. Subsequently, the mice received 400?ng of Pertussis toxin (PTX) (List Biological Labs, Campbell, CA, USA, cat# 180) intraperitoneally at 2 and 24?h post-injection of the emulsion. Intravenous injection of Cy5-labeled nanoparticles at a dose of 40?mg per kg was;reunds adjuvant (CFA) (Chondrex, Woodinville, WA, USA, cat# 7001), consisting of 4?mg/mL of heat-killed M. Tuberculosis H37 RA. Subsequently, the mice received 400?ng of Pertussis toxin (PTX) (List Biological Labs, Campbell, CA, USA, cat# 180) intraperitoneally at 2 and 24?h post-injection of the emulsion. Intravenous injection of Cy5-labeled nanoparticles at a dose of 40?mg per kg was ... reunds adjuvant (CFA) (Chondrex, Woodinville, WA, USA, cat# 7001), consisting of 4?mg/mL of heat-killed M. Tuberculosis H37 RA. Subsequently, the mice received 400?ng of Pertussis toxin (PTX) (List Biological Labs, Campbell, CA, USA, cat# 180) intraperitoneally at 2 and 24?h post-injection of the emulsion. Intravenous injection of Cy5-labeled nanoparticles at a dose of 40?mg per kg was

December 29, 2024

Pharmaceuticals

Kim, S;Jeong, S;Kim, J;Kim, M;Lee, W;Lee, C;Yook, T;Yang, G;

Product: Unspecified List Labs LPS

e serum obtained Invitrogen (Carlsbad, CA, USA), 10,000 units/mL penicillin, and 10,000 ?g/mL streptomycin. 3.2. REAGENTS We obtained purified lipopolysaccharides (LPS) from Escherichia coli from List Bio-logical Laboratory (Campbell, CA, USA), which we dissolved in endotoxin-free water. We obtained MSU from Invivogen (Carlsbad, CA, USA), and we crystallized it in phosphate-buffered saline (PB

December 23, 2024

Plos One

Yibcharoenporn, C;Kongkaew, T;Worakajit, N;Khumjiang, R;Saetang, P;Satitsri, S;Rukachaisirikul, V;Muanprasat, C;

Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

SA). Antibodies against CFTR and ?-actin were purchased from Cell Signaling Technology (Boston, MA, USA). Cholera toxins from _Vibrio cholerae_ (product no.100B: Lot no. 10071A2) were purchased from List Biological Laboratories (Campbell, CA, USA). Other compounds and chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA). CELL CULTURE T84 cells at passage 10-30 from the American Ty ... SA). Antibodies against CFTR and ?-actin were purchased from Cell Signaling Technology (Boston, MA, USA). Cholera toxins from _Vibrio cholerae_ (product no.100B: Lot no. 10071A2) were purchased from List Biological Laboratories (Campbell, CA, USA). Other compounds and chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA). CELL CULTURE T84 cells at passage 10-30 from the American Ty;USA). Antibodies against CFTR and ?-actin were purchased from Cell Signaling Technology (Boston, MA, USA). Cholera toxins from Vibrio cholerae (product no.100B: Lot no. 10071A2) were purchased from List Biological Laboratories (Campbell, CA, USA). Other compounds and chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cell culture T84 cells at passage 10-30 from the American Typ ... USA). Antibodies against CFTR and ?-actin were purchased from Cell Signaling Technology (Boston, MA, USA). Cholera toxins from Vibrio cholerae (product no.100B: Lot no. 10071A2) were purchased from List Biological Laboratories (Campbell, CA, USA). Other compounds and chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cell culture T84 cells at passage 10-30 from the American Typ

December 23, 2024

Plos Neglected Tropical Diseases

Jiranantasak, T;Bluhm, A;Chabot, D;Friedlander, A;Bowen, R;McMillan, I;Hadfield, T;Hartwig, A;Blackburn, J;Norris, M;

Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis

0-fold dilutions in DMEM supplemented with 10% FBS and 10 mM HEPES. Pooled serum samples from unvaccinated animals served as a control. The diluted serum samples were then incubated with 50 ng/ml PA (List Labs, Campbell, CA, USA) and 160 ng/ml LF (List Labs, Campbell, CA, USA) for 30 min before adding to RAW264.7 cells. The mixtures of cells, serum, and toxin were incubated for 6 h. After incubati;0-fold dilutions in DMEM supplemented with 10% FBS and 10 mM HEPES. Pooled serum samples from unvaccinated animals served as a control. The diluted serum samples were then incubated with 50 ng/ml PA (List Labs, Campbell, CA, USA) and 160 ng/ml LF (List Labs, Campbell, CA, USA) for 30 min prior adding into RAW264.7 cells. The mixtures of cells, serum, and toxin were incubated for 6 h. After incubat

December 20, 2024

Jci Insight

King, EM;Zhao, Y;Moore, CM;Steinhart, B;Anderson, KC;Vestal, B;Moore, PK;McManus, SA;Evans, CM;Mould, KJ;Redente, EF;McCubbrey, AL;Janssen, WJ;

Product: LPS from Escherichia coli O55:B5

Tamoxifen was suspended in corn oil and delivered at a concentration of 0.2 mg/g per dose. Animals were rested for 4 weeks after the final tamoxifen dose (13, 38). E. coli LPS O55:B5 (List Biological Laboratories) was instilled into the tracheas of mice sedated with isoflurane (Baxter) at a concentration of 20 ?g in 50 ?L of PBS using a modified gel-loading tip with direct laryngoscopy (13)

December 19, 2024

Science (New York, N.Y.)

Canesso, MCC;Castro, TBR;Nakandakari-Higa, S;Lockhart, A;Luehr, J;Bortolatto, J;Parsa, R;Esterházy, D;Lyu, M;Liu, TT;Murphy, KM;Sonnenberg, GF;Reis, BS;Victora, GD;Mucida, D;

Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Eight days after oral administration of OVA, 1 mg OVA + 20 ?g cholera toxin (100B; List Biological) in 0.2 M sodium bicarbonate were provided by gavage once per week

December 18, 2024

Frontiers In Molecular Neuroscience

Xia, H;Jerde, TJ;Fehrenbacher, JC;

Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Diphtheria toxin (DTX; List Biological Laboratories, Product #150) was reconstituted to 20??g/mL in sterile 0.9% saline, aliquoted, and stored at ?80°C until used for injections

Bacterial Toxin Research Citations

Development of a Recombinant Fusion Vaccine Candidate Against Lethal Clostridium botulinum Neurotoxin Types A and B

DAG-MAG-ΒHB: A Novel Ketone Diester Modulates NLRP3 Inflammasome Activation in Microglial Cells in Response to Beta-Amyloid and Low Glucose AD-like Conditions

A core glycolipid vaccine elicits cross-reactive antibodies against Salmonella spp. and protects against invasive nontyphoidal Salmonella disease in mice

NET formation-mediated in situ protein delivery to the inflamed central nervous system

Zanthoxylum piperitum Attenuates Monosodium Urate-Induced Gouty Arthritis: A Network Pharmacology Investigation of Its Anti-Inflammatory Mechanisms

Inhibition of CFTR-mediated intestinal chloride secretion by nornidulin: Cellular mechanisms and anti-secretory efficacy in human intestinal epithelial cells and human colonoids

Toxin and capsule production by Bacillus cereus biovar anthracis influence pathogenicity in macrophages and animal models

Gpnmb and Spp1 mark a conserved macrophage injury response masking fibrosis-specific programming in the lung

Identification of antigen-presenting cell-T cell interactions driving immune responses to food

Sensory innervation in the prostate and a role for calcitonin gene-related peptide in prostatic epithelial proliferation