Bacterial Toxin Research Citations

September 1, 2024

Iscience

OSell, J;Cirulli, V;Pardike, S;Aare-Bentsen, M;Sdek, P;Anderson, J;Hailey, D;Regier, M;Gharib, S;Crisa, L;

Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Diphtheria Toxin from Corynebacterium Diphtheria (unnicked) List Biological Laboratories Inc Cat# 150 Liberase TL Roche-Millipore Cat#05401020001

August 29, 2024

Molecular Immunology

Mani, NL;Weinberg, SE;Chaudhuri, S;Montauti, E;Tang, A;Iyer, R;Fang, D;

Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

either pH 6.5, pH 7.2, or pH 6.5 with 100uM sodium-formate. 2 ×106 Treg cells 2, were retro-orbitally injected at a volume of 100 uL in PBS. Control animals received 100 uL PBS alone. FoxP3DTR mice to receive transferred Treg cells were treated with 50?mg/kg diphtheria toxin (DT) (List Labs, 150) starting from 5 days prior to tumor inoculation and for every 3 days until time of euthanization. Mice were monitored daily for tumor growth. Tumor volume was measured through digital calipers along the x, y, and z axes and calculated as (xyz)/2. Mice were to be euthanized if tumors exceeded size limit agreed by the Institutional Review Board (IRB), however no animal reached this volume during the duration of the experiment. On day 14, mice

August 28, 2024

Biorxiv

Brune, Z;Lu, A;Moss, M;Brune, L;Huang, A;Matta, B;Barnes, B;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

atories). On day 0 (D0) and 1 day after (D1) immunization, mice were intraperitoneally injected with 200 ng of pertussis toxin (Cat#: 180, List Biological Laboratories). All mice were examined and graded daily for neurological signs in a blinded manner as previously described. For the tail: 0, no disease; 1, half paralyzed; 2, full paralysis. For each hind and forelimb assessed separately: 0, no disease; 1, weak or altered gait; 2, paresis; 3, limb

August 26, 2024

Journal Of Immunology (Baltimore, Md. : 1950)

Gibbs, LC;Oviedo, JM;Ondigo, BN;Fairfax, KC;

Product: Tetanus Toxoid from Clostridium tetani

with FlowJo. Tetanus toxoid fluorophore conjugation Tetanus toxoid (191B; List Labs) was conjugated using the Lightning Link FITC conjugation kit (ab102884; Abcam) per manufacturers instruction. OVA conjugated to biotin (ab201795; Abcam) was used in conjunction with the conjugated tetanus toxoid to control for nonspecific B cell binding. The decoy was incubated with streptavidin-BV786 (563858; BD Biosciences) for 10 min at room temperature before mixing with the conjugated tetanus toxoid Ab. Cells were sta

August 23, 2024

Biochemical Pharmacology

Chen, H;Song, J;Zeng, L;Zha, J;Zhu, J;Chen, A;Liu, Y;Dong, Z;Chen, G;

Product: ULTRA PURE LPS from Escherichia coli O111:B4

dose LPS injection method to establish a CKD animal model as described previously [27] . Male 6-8 weeks old C57BL/6J/Sja mice (Hunan Slack King Experimental Animal Company) were maintained under specific pathogen-free conditions. Ultra Pure LPS (Escherichia coli 0111:B4, LIST Biological Laboratories, Campbell, USA) was dissolved in sterile normal saline and injected intraperitoneally (1 mg/kg) every two days to induce chronic endotoxemia for 4 weeks, followed by an injection every two weeks until the 12th week. A subgroup of the mice received a high-salt diet (4 % NaCl in chow plus 1 % in the drinking water) or treatment with

August 22, 2024

Biorxiv : The Preprint Server For Biology

Luebbers, A;Janicot, R;Zhao, J;Philibert, CE;Garcia-Marcos, M;

Product: Pertussis Toxin from B. pertussis (in Glycerol)

irus-containing medium was replaced by fresh medium 6 hr later. In some conditions, the change of media was accompanied by the addition of 0.1 ?g/ml pertussis toxin (List Biological Labs, cat#179A) to wells. Approximately 18-22 hr after the change to fresh medium, cells were washed and gently scraped in room temperature PBS, centrifuged (5 minutes at 550 × g), and resuspended in 750 ?l of BRET buffer. Fifty ?l of cells were added to a white opaque 96-well plate, followed by addition of 50 ?l of BRET buffer and the nanoluciferase substrate Nano-Glo (final dilution 1:200) before measuring luminesc

August 21, 2024

Nature Communications

Glangetas, C;Guillaumin, A;Ladevèze, E;Braine, A;Gauthier, M;Bonamy, L;Doudnikoff, E;Dhellemmes, T;Landry, M;Bézard, E;Caille, S;Taupignon, A;Baufreton, J;Georges, F;

Product: Anti-Cholera Toxin B Subunit (Goat)

2 primary antibody (1/500; Abcam ab18465), or with a rat anti-MBP (1/500, Merckmillipore), a guinea pig anti-NeuN/Fox3 (1/1000, cat 26604, Synaptic system), or with a goat anti-CTb antibody (1/10000, list labs #703), or a rabbit anti-GFP primary antibody (1/1000; Millipore, AB3080), a mouse anti-GAD67 primary antibody (1/500; Millipore MAB5406), a guinea pig anti-PV primary antibody (1/1000, synap

August 20, 2024

Cell Metabolism

Cordeiro, B;Ahn, JJ;Gawde, S;Ucciferri, C;Alvarez-Sanchez, N;Revelo, XS;Stickle, N;Massey, K;Brooks, DG;Guthridge, JM;Pardo, G;Winer, DA;Axtell, RC;Dunn, SE;

Product: Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Heat-killed Mycobacterium tuberculosis H37Ra Difco Laboratories Difco Laboratories Cat#: DF3114-33-8 Bordetella pertussis toxin List Biologicals List Biologicals Cat#: 181 Luxol fast blue Sigma Sigma Cat#:L0294 Collagenase IV Sigma Sigma Cat#:C5138-500MG Collagenase D Roche Roche Cat#: 11088866001 DNAse I Sigma Sigma Cat#:11284932001 Percoll Fisher Scientific Fisher Scientific Cat#: 45-001-747 FBS Corning Corning Cat#: 35-077-CV EDTA Bioshop

August 16, 2024

Chemrxiv

Ji, J;Kawano, R;

Product:

3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Liss Rhod 80 PE, Avanti Polar Lipids, USA); 1-octanol (SigmaAldrich Co., USA); ?-hemolysin (?HL; Sigma-Aldrich, 81 St. Louis, MO, USA, and List Biological Laboratories, Campbell, CA, USA), Alexa 488 (Thermo Fisher 82 Scientific, USA), Glucose and sucrose (KISHIDA, JAPAN)

August 15, 2024

The Journal Of Clinical Investigation

He, J;Chen, Y;Ding, H;Zhou, JA;Xing, Z;Yang, X;Fan, Q;Zuo, Y;Wang, T;Cheng, J;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

tion. Six- to 8-week-old Vegfb WT and cKO female mice were used in the EAE model. EAE was induced by subcutaneous immunization with MOG35-55 peptides (200 ?g/mouse, catalog T510219, Sangon Biotech) emulsified in complete Freunds adjuvant (catalog F5881, Sigma-Aldrich), followed by administration of pertussis toxin (200 ng/mouse, PTX, List Biological Laboratories Inc.) on days 0 and 2 as described above. Clinical signs of EAE were assessed according to the following score: 0, no signs of disease; 1, loss of tone in the tail; 2, hind limb paresis; 3, hind limb paralysis; 4, tetraplegia; 5, moribund. H&E an

Bacterial Toxin Research Citations

Disruption of perinatal myeloid niches impacts the aging clock of pancreatic ? cells

Acidity induces durable enhancement of Treg cell suppressive functions for tumor immune evasion

IRF5 mediates adaptive immunity via altered glutamine metabolism, mTORC1 signaling and post-transcriptional regulation following T cell receptor activation

Maternal Helminth Infection Causes Dysfunctional B Cell Development in Male Offspring

Dietary sodium modulates mTORC1-dependent trained immunity in macrophages to accelerate CKD development

Direct detection of endogenous G?i activity in cells with a sensitive conformational biosensor

A population of Insula neurons encodes for social preference only after acute social isolation in mice

Obesity intensifies sex-specific interferon signaling to selectively worsen central nervous system autoimmunity in females

Microfluidic generation of cell-sized liposomes using a budding strategy

Autocrine VEGF-B signaling maintains lipid synthesis and mitochondrial fitness to support T cell immune responses