Citations

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4664 total record number 37 records this year

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4664 citations found

The therapeutic effect of gold clusters in an EAE model of multiple sclerosis by regulating T cell differentiation

He, Z;Lai, J;Wang, H;He, Y;Zhang, C;Gao, L;Huang, H;Zheng, L;Hao, J;Gao, X;Gao, F;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Fate mapping of Spp1 expression reveals age-dependent plasticity of disease-associated microglia-like cells after brain injury

Lan, Y;Zhang, X;Liu, S;Guo, C;Jin, Y;Li, H;Wang, L;Zhao, J;Hao, Y;Li, Z;Liu, Z;Ginhoux, F;Xie, Q;Xu, H;Jia, J;He, D;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Characterization of pathological stages in a mouse model of progressive multiple sclerosis

Hamano, S;Yoshimizu, T;Mori, M;Iida, A;Yamashita, T;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

A peptide from the Japanese encephalitis virus failed to induce the production of anti-N-methyl-d-aspartate receptor antibodies via molecular mimicry in mice

Luo, H;Yang, J;Yang, X;Han, Z;Fang, Z;Huang, D;Gui, J;Ding, R;Chen, H;Cheng, L;Ma, J;Jiang, L;

Product: Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

  • 2.3. Study design and active immunization

    Action immunization was conducted as previously reported [16]. The peptides were dissolved in saline (2 mg/ml), and then fully
    emulsified in an equal volume of complete Freund’s adjuvant containing 1 mg/ml heat-killed Mycobacterium tuberculosis H37Ra
    [(CFA), #F5881, Sigma Aldrich, USA]. Mice were randomly assigned to experimental, negative control, or positive control groups.
    Each mouse received a subcutaneous injection of emulsion (200 μl containing 200 μg of peptide) at four sites on the back, which was
    repeated three times at 2, 4, and 6 weeks (Fig. 1). The GluN1359-378 peptide emulsion was injected as the positive control. For the sham
    groups, an equal volume of saline emulsified in CFA was used in place of the peptide. Two hundred nanograms of pertussis toxin (1 ng/μl, #181, List Biological Laboratories, USA) was intraperitoneally administered to each mouse at the time of the last immunization and 48 h later.

    Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
    Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
    Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
    Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Defined microbial communities and their soluble products protect mice from Clostridioides difficile infection

Douchant, K;He, SM;Noordhof, C;Greenlaw, J;Vancuren, S;Schroeter, K;Allen-Vercoe, E;Sjaarda, C;Vanner, SJ;Petrof, EO;Sheth, PM;Guzman, M;

Product: Toxin A from Clostridium difficile

  • Silver staining and western blot analysis

    TcdA (1 μg) (List Biological Laboratories, Inc. California, USA) was incubated with DMC-4 (35 μl) for 1 h at 37 °C. Samples were loaded using 6X SDS loading buffer and heated at 95 °C for 10 min. All samples were loaded on to gradient SDS-PAGE gels (4-15%) after boiling. Silver staining was performed using the Pierce Silver Stain Kit as per the manufacturer’s instructions (Thermo Fisher Scientific. USA).

    TcdA and TcdB (100 ng) were incubated with DMC-4 (35 μL) for 1 h at 37 °C. Samples were loaded using 6X SDS loading buffer and heated at 95 °C for 10 min. All samples were loaded into gradient SDS-PAGE gels (4-15%) after boiling. …

    Product #152C – Toxin A from Clostridium difficile

Investigation into Cardiac Myhc-α 334–352-Specific TCR Transgenic Mice Reveals a Role for Cytotoxic CD4 T Cells in the Development of Cardiac Autoimmunity

Sur, M;Rasquinha, M;Mone, K;Massilamany, C;Lasrado, N;Gurumurthy, C;Sobel, R;Reddy, J;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Identification of a novel perifornical-hypothalamic-area-projecting serotonergic system that inhibits innate panic and conditioned fear responses

Bernabe, CS;Caliman, IF;de Abreu, ARR;Molosh, AI;Truitt, WA;Shekhar, A;Johnson, PL;

Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Thymic-Epithelial-Cell-Dependent Microenvironment Influences Proliferation and Apoptosis of Leukemic Cells

Patel, S;Zhdanovskaya, N;Sergio, I;Cardinale, A;Rosichini, M;Varricchio, C;Pace, E;Capalbo, C;Locatelli, F;Macone, A;Velardi, E;Palermo, R;Felli, M;

Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

  • 4.1. Cell Culture and Generation of Human TEC Culture

    … Cells were cultured in a humidified atmosphere of 5% CO2 in a medium composed of DMEM:Ham’s F-12 (3:1 ratio), 10% fetal calf serum (FCS) (ThermoFisher, Waltham, MA, USA), adenine (0.18 mM, Sigma-Aldrich, St. Louis, MO, USA), hydrocortisone (0.4 μg/mL, Sigma-Aldrich, St. Louis, MO, USA), insulin (5 μg/mL, Eli Lilly, Indianapolis, Indiana), cholera toxin (0.1 nM, List Labs, Campbell, CA, USA), glutamine (4 mM, ThermoFisher, Waltham, MA, USA), triiodothyronine (2 nM, Sigma), and antibiotics. Epidermal growth factor (10 ng/mL, Austral Biologicals, San Ramon, CA, USA) was added to the medium after 48 h of culture. …

    Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

The choroid plexus maintains ventricle volume and adult subventricular zone neuroblast pool, which facilitates post-stroke neurogenesis

Taranov, A;Bedolla, A;Iwasawa, E;Brown, F;Baumgartner, S;Fugate, E;Levoy, J;Crone, S;Goto, J;Luo, Y;

Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Maternal n-3 enriched diet reprograms neurovascular transcriptome and blunts inflammation in neonate

Chumak, T;Jullienne, A;Joakim Ek, C;Ardalan, M;Svedin, P;Quan, R;Salehi, A;Salari, S;Obenaus, A;Vexler, Z;Mallard, C;

Product: ULTRA PURE LPS from Escherichia coli O55:B5

  • Diet and treatment

    Breeding cages were randomly assigned to one of the experimental isocaloric diets (AIN93G, BioServ): standard (Stand, BioServ #S7628), n-3 long chain (LC)-PUFA enriched (n-3, BioServ #S7630; soybean oil replaced with Menhaden Fish oil) or n-6 LC-PUFA supplemented (n-6, BioServ #S7629; based on corn oil). The detailed composition of the diets is presented in our previous publication (Chumak et al., 2022). The day of birth was defined as postnatal day (P) 0. At P9 pups received a single intraperitoneal injection of lipopolysaccharide (LPS from E. coli O55:B5, 1mg/kg, Cat# 423, List labs) or equivalent volume of saline. The dams remained on the experimental diet until offspring sacrifice (P10-P12). Efforts were taken to include equal number of pups of both sexes in each experimental group.

    Product #423 – ULTRA PURE LPS from Escherichia coli O55:B5