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3951 citations found

Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

  • 2.4.1. Retrograde labeling

    … A pulled glass pipette (~20 μm outer tip diameter) was attached to the arm of the stereotaxic apparatus. A solution of 0.25% CTB (List Biological Labs, Campbell, CA, USA) in sodium phosphate buffer (pH 7.5) was backfilled through the pipette tip using negative pressure, then a wire connected to a current source (Stoelting) was inserted into the tracer solution. …

    Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Connections of the mouse subfornical region of the lateral hypothalamus (LHsf)

Ugur, M;Doridot, S;la Fleur, SE;Veinante, P;Massotte, D;

Product: Anti-Cholera Toxin B Subunit (Goat)

Activation of Transcription Factor 4 in Dendritic Cells Controls Th1/Th17 Responses and Autoimmune Neuroinflammation

Manoharan, I;Swafford, D;Shanmugam, A;Patel, N;Prasad, PD;Thangaraju, M;Manicassamy, S;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Regulatory T cells function in established systemic inflammation and reverse fatal autoimmunity

Hu, W;Wang, ZM;Feng, Y;Schizas, M;Hoyos, BE;van der Veeken, J;Verter, JG;Bou-Puerto, R;Rudensky, AY;

Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Species-Specific Endotoxin Stimulus Determines Toll-Like Receptor 4- and Caspase 11-Mediated Pathway Activation Characteristics

Ernst, O;Khan, MM;Oyler, BL;Yoon, SH;Sun, J;Lin, FY;Manes, NP;MacKerell, AD;Fraser, IDC;Ernst, RK;Goodlett, DR;Nita-Lazar, A;

Product: LPS from Escherichia coli J5 (Rc)

  • Top-down lipopolysaccharide structural analysis using multistage MS (MS/MS and MS3)

    To determine the structures and validate the purity of different rough-type LPS preparations, top-down LPS structural elucidation was performed as described previously (27). Highly purified LPS of Bordetella pertussis 165 strain and Escherichia coli J5 strain were obtained from List Biological Laboratories, Inc. (Campbell, CA). LPS samples were dissolved at an approximate concentration of 20 μg ml−1 in 2-propanol/water (50:50 [vol/vol]). …

    Product #301 – LPS from Escherichia coli J5 (Rc)

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

ZEB1 promotes pathogenic Th1 and Th17 cell differentiation in multiple sclerosis

Qian, Y;Arellano, G;Ifergan, I;Lin, J;Snowden, C;Kim, T;Thomas, JJ;Law, C;Guan, T;Balabanov, RD;Kaech, SM;Miller, SD;Choi, J;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

  • EAE induction and activity

    Eight to twelve weeks-old mice were injected subcutaneously with 100mL of an emulsion containing 200mgofMycobacterium tuber-culosisH37Ra (Cat# DF3114-33-8, Difco) and 200mg of MOG35–55(Cat# MOG3555-P2, Genemed Synthesis) distributed over threesites on the flank. On day 0 and 2 after immunization, 200 ng pertussis toxin (Cat# 180, List Biological Laboratories) were adminis-tered intraperitoneally (i.p.). …

    Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Inhibition of elastase enhances the adjuvanticity of alum and promotes anti-SARS-CoV-2 systemic and mucosal immunity

Kim, E;Attia, Z;Woodfint, RM;Zeng, C;Kim, SH;Steiner, HE;Shukla, RK;Liyanage, NPM;Ghimire, S;Li, J;Renukaradhya, GJ;Satoskar, AR;Amer, AO;Liu, SL;Cormet-Boyaka, E;Boyaka, PN;

Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis

CD11b+ lung dendritic cells at different stages of maturation induce Th17 or Th2 differentiation

Izumi, G;Nakano, H;Nakano, K;Whitehead, GS;Grimm, SA;Fessler, MB;Karmaus, PW;Cook, DN;

Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

  • Allergic sensitization and mouse model of asthma

    For allergic sensitization, mice were lightly anesthetized with isoflurane and given two oropharyngeal (o.p.) instillations, 1 week apart, of 100 μg LPS-free OVA (Worthington Biomedical) with 10 μL HDE or 100 ng LPS (Millipore Sigma) in a total volume of 50 μL in PBS (HDE/OVA or LPS/OVA)30. The HDE was prepared as previously described32,59. Briefly, vacuumed dust samples from homes in North Carolina were passed through a coarse sieve, then extracted at 100 mg/mL with PBS at 4 °C with overnight mild agitation. The samples were centrifuged to remove insoluble debris, and supernatants were sterilized by passage through a 0.22 μm filter (Millipore Sigma). Endotoxin concentration was 50 ng LPS/10 μL HDE, as measured by a Limulus Amebocyte Lysate assay (Lonza, catalog #50-648U). In some experiments, 100 μg Alexa Fluor (AF) 647-conjugated OVA or 10 μg DQ-OVA (ThermoFisher Scientific) was used to analyze antigen uptake60. In some experiments, DTX (20 ng/g body weight) (List Biological Laboratories) was injected into peritoneal cavity of zDC-DTA mice prior to airway sensitization …

    Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Functional diagnostics using fresh uncultured lung tumor cells to guide personalized treatments

Talwelkar, SS;Mäyränpää, MI;Søraas, L;Potdar, S;Bao, J;Hemmes, A;Linnavirta, N;Lømo, J;Räsänen, J;Knuuttila, A;Wennerberg, K;Verschuren, EW;

Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

  • CR cultures

    Both murine and human EpCAM+ cells were propagated using a Conditional Reprogramming (CR) culture protocol. In brief, EpCAM+ cells were plated on irradiated (30 gray) 3T3 cells in F-medium composed of 1:3 v/v DMEM: F-12 nutrient HAM supplemented with 5% FBS, 10 ng/ml EGF (BD Biosciences; 354052), 5 μg/ml insulin (Sigma; I2643), 24 μg/ml adenine (Sigma; A2786), 0.4 μg/ml hydrocortisone (Sigma; H4001), 10 ng/ml cholera toxin (List Biological laboratory; 100B), and 10 μM ROCK inhibitor (Y-27632; ENZO). All CR cultures were maintained at 37°C and 5% CO2. When CR cultures reached 80% confluence, they were differentially trypsinized using a two-step procedure the first to remove loosely attached feeder cells, and the second to trypsinize epithelial cells.

    Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae