Citations

2.2. EAE induction and treatment
EAE was induced according to previously established methods [17,18]. Briefly, mice were subcutaneously immunized with 200 mg MOG 35–55 peptide emulsified in complete Freund’s adjuvant containing 0.4 mg Mycobacterium tuberculosis H37 Ra (BD Difco) and intraperitoneally injected with 200 ng pertussis toxin (List Biological Laboratories) on days 0 and 2 post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

4.1. Cell Line and Reagents for Development of the AP-1 Reporter Assay
Murine hepatoma Hepa1c1c7 cells (CRL-2026™) purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) were cultured in an alpha minimum essential medium (MEM), supplemented with 10% FBS, 100 IU/mL penicillin, 100 µg/mL streptomycin, and 2 mM L-glutamine. Cultures were maintained at 37 °C in an incubator with 5% CO2. Lyophilized recombinant protective antigen (PA) and lethal factor (LF) were purchased from List Biological Laboratories, Inc. (Campbell, CA, USA), and reconstituted in sterile water to make stock solutions with a final concentration of 1 mg/mL for LF and 2 mg/mL for PA. The stock solutions were then diluted 10 times with 50% glycerol in sterile water to make stock solutions with a final concentration of 0.1 mg/mL for LF and 0.2 mg/mL for PA. …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

… Pertussis toxin (200 ng/mouse, List Biological Laboratories, Inc., Campbell, CA, USA) in sterile saline was administered via the tail vein on day 0 and day 2 after MOG35-55 immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Limbus processing procedures

… 0.1 nM cholera toxin (List Biological Laboratories, Campbell, California, USA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Pertussis toxin (List Biologicals) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Lipopolysaccharide, Bleomycin, House Dust Mite Administration

Lipopolysaccharide (LPS, Escherichia coli O111: B4, List Biological Laboratories, Campbell, CA, USA) was administered by oropharyngeal aspiration under isoflurane anesthesia at a dose of 2.0 mg/kg of mouse body weight in 50μL of PBS. …

• Product #201 – LPS from Escherichia coli O111:B4

… Pertussis toxin List Biological Laboratories Inc 180 …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Serological B. pertussis–Specific Immunoglobulin G by ELISA

Levels of antigen-specific serum immunoglobulin (Ig) G were measured as described elsewhere [21]. Nunc 96-well plates were coated with 0.2 µg/mL pertussis toxin (PT), 0.5 µg/mL filamentous hemagglutinin adhesin, 2 µg/mL pertactin, 0.2 µg/mL fimbriae 2 and 3, or 10 µg/mL lipooligosaccharide. For adenylate cyclase toxin (ACT), Bordetella intermediate protein A (BipA), and HKBp, the plates were coated with 1 µg/mL ACT, 1 µg/mL BipA, or HKBp at an optical density of 0.9 at 600 nm. All pertussis antigens were obtained from List Biologicals. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

• Product #186 – Fimbriae 2/3 from B. pertussis

• Product #187 – Pertactin from B. pertussis (69 kDa Protein)

• Product #188L – Adenylate Cyclase Toxin, Recombinant from B. pertussis

Immunizations

8- to 12-week-old male and female mice were immunized subcutaneously with a 100 μl emulsion of 100 μg α-syn32-46 peptide (A&A peptides; San Diego, CA) dissolved in PBS with Complete Freund Adjuvant (CFA) containing 200 μg M. tuberculosis H37Ra (BD Difco Adjuvant, Fisher Scientific, Cat #DF0638-60-7; Waltham, MA). The day of immunization was designated as day 0. Mice received a boost with the same immunization dose two weeks later. Mice received intravenous injections of 400 ng of B. pertussis toxin (List Biological Laboratories, Cat #181; Campbell, CA) 14- and 16-days post-initial immunization (day 0 and 2 post boost). …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Methods
In a monocentric, randomized, double-blind, placebo-controlled, parallel-group, phase I trial, 57 healthy, smoking subjects were treated for 28 days with either oral BI 1026706 100 mg bid or placebo. At day 21, turbo-inversion recovery magnitude magnetic resonance imaging (TIRM MRI) was performed. On the last day of treatment, pre-challenge bronchoalveolar lavage fluid (BAL) and biopsies were sampled, followed by segmental LPS challenge (40 endotoxin units/kg body weight) and saline control instillation in different lung lobes. Twenty-four hours later, TIRM MRI was performed, then BAL and biopsies were collected from the challenged segments. In BAL samples, cells were differentiated for neutrophil numbers as the primary endpoint. Other endpoints included assessment of safety, biomarkers in BAL (e.g. interleukin-8 [IL-8], albumin and total protein), B1R expression in lung biopsies and TIRM score by MRI as a measure for the extent of pulmonary oedema.

… Next, Lipopolysaccharide (Endotoxin from E. coli Type O113 provided by National Institutes of Health Clinical Center, Bethesda, Maryland, USA and produced by List Biological Laboratories Inc., Campbell, California, USA) at a dose of 40 endotoxin units (E.U.)/kg …

• Product #9433 – GMP LPS Lipopolysaccharide List™ Hpt™ From Escherichia Coli Type O113