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4216 total record number 177 records this year

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4216 citations found

ORAI3 is dispensable for store-operated Ca2+ entry and immune responses by lymphocytes and macrophages

Wang, L;Noyer, L;Wang, YH;Tao, AY;Li, W;Zhu, J;Saavedra, P;Hoda, ST;Yang, J;Feske, S;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

MW‑9, a chalcones derivative bearing heterocyclic moieties, attenuates experimental autoimmune encephalomyelitis via suppressing pathogenic TH17 cells

Liu, B;Mao, Z;Yin, N;Gu, Q;Gu, Q;Qi, Y;Li, X;Yang, H;Wu, Z;Zou, N;Ying, S;Wan, C;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Anemoside B4 ameliorates experimental autoimmune encephalomyelitis in mice by modulating inflammatory responses and the gut microbiota

Zou, M;Chen, FJ;Deng, LR;Han, Q;Huang, CY;Shen, SS;Tomlinson, B;Li, YH;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Product: Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Discovery of new macrophage M2 polarization modulators as multiple sclerosis treatment agents that enable the inflammation microenvironment remodeling

Che, J;Li, D;Hong, W;Wang, L;Guo, Y;Wu, M;Lu, J;Tong, L;Weng, Q;Wang, J;Dong, X;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Blockade of the Adenylate Cyclase Toxin Synergizes with Opsonizing Antibodies to Protect Mice against Bordetella pertussis

DiVenere, AM;Amengor, D;Silva, RP;Goldsmith, JA;McLellan, JS;Maynard, JA;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Lipopolysaccharide affects energy metabolism and elevates nicotinamide N-methyltransferase level in human aortic endothelial cells (HAEC)

St?pi?ska, O;Dymkowska, D;Mateuszuk, ?;Zab?ocki, K;

Product: ULTRA PURE LPS from Escherichia coli O111:B4

  • 2.1. Cell culture and treatment

    HAECs were purchased from Lonza. Cells were grown in EGM BulletKit®−2 (Lonza) at 37ºC in an atmosphere of 5% CO2 and 95% air. The culture medium was composed exactly as recommended by the supplier (Lonza). Confluent cells were exposed to 100 ng/ml LPS (Escherichia coli O111:B4, List Biological Laboratories, #421) for a period within 0.5 h to 24 h as indicated in figure legends. In all experiments, cells of the third passage were used.

    Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

AMPK-Nrf2 Signaling Pathway in Phrenic Motoneurons following Cervical Spinal Cord Injury

Michel-Flutot, P;Efthimiadi, L;Djerbal, L;Deramaudt, T;Bonay, M;Vinit, S;

Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

  • 2.2. Animal Groups and Surgical Preparation

    In this study, 63 animals were used, divided into 9 groups: control (n = 4), Sham animals (at 1 h (n = 6), 1 day (n = 7), 3 days (n = 7), and 7 days (n = 7) post-surgery), and C2-injured animals (at 1 h (n = 8), 1 day (n = 8), 3 days (n = 8), and 7 days (n = 8) post-injury). None of the C2-injured animals died during or after the procedure, however one Sham animal died at 1 h due to a technical issue. All animals (except n = 4 of each group) received an intrapleural injection with the retrograde tracer Cholera toxin B fragment (CTB, List Biologicals, Campbell, CA, USA) to identify phrenic motor neurons in the cervical spinal cord, similar to that described previously [41,42,43,44]. Briefly, each rat was anaesthetized with isoflurane (Iso-vet, Piramal, Voorschoten, Netherlands; ~1.5% in O2), placed in the supine position on a surgical table while spontaneously breathing into a face mask. The lateral sides of the rib cage were shaved and gently palpated to identify the fifth intercostal space at the anterior axillary line. A sterilized 26G needle connected to a 50 μL Hamilton syringe was used to inject 25 μL of a 0.2% CTB (dissolved in sterile injectable saline) between the 5th and the 6th ribs into the pleural space on each side. …

    Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

IκBζ controls IL-17-triggered gene expression program in intestinal epithelial cells that restricts colonization of SFB and prevents Th17-associated pathologies

Yamazaki, S;Inohara, N;Ohmuraya, M;Tsuneoka, Y;Yagita, H;Katagiri, T;Nishina, T;Mikami, T;Funato, H;Araki, K;Nakano, H;

Product: LPS from Escherichia coli O111:B4

The expression of nerve growth factor in healthy and inflamed equine chondrocytes analysed by capillary western immunoassay

Nyström, S;Kendall, A;Adepu, S;Lindahl, A;Skiöldebrand, E;

Product: LPS from Escherichia coli O55:B5

  • 2.1. Chondrocyte isolation and culture

    … Samples were placed in sterile saline (0.9% NaCl) solution with gentamicin sulfate (50 mg/l) and amphotericin B (250 μg/ml). The cartilage samples were transported chilled (approx. 5 °C) to the laboratory. Isolation and expansion of chondrocytes were performed as previously described (Ley et al., 2011). Briefly, the chondrocytes were expanded to passage 1 and then seeded at 20,000 cells/cm2 in chondrogenic medium to maintain the phenotype. On day 4, cells were stimulated with LPS (10 ng/ml, Escherichia coli 055:B5; List Biological Laboratories, Campbell, CA, USA) or kept untreated (controls) for 24 h. Cells were grown to confluence and harvested on day 5 and immediately frozen and stored at −80 °C until further analyses.

    Product #203A – LPS from Escherichia coli O55:B5