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3951 citations found

Optic Nerve Engraftment of Neural Stem Cells

Do, JL;Allahwerdy, S;David, RCC;Weinreb, RN;Tuszynski, MH;Welsbie, DS;

Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

  • In Vivo Optic Nerve Transection and NSC Transplant

    … Three days before tissue collection, animals received intravitreal injections of cholera toxin subunit B (CTB; 2 µL, 1 µg/µL; List Biological Labs, Campbell, CA, USA) to anterogradely label RGC axons. Animals were perfused transcardially with cold PBS, followed by 4% PFA, and post fixed in 4% PFA overnight. The eyes were dissected and stored in PBS. The optic nerves were dissected and cryoprotected in 30% sucrose.

    Retina and Optic Nerve Immunohistochemistry

    Primary antibodies to CTB (1:1000; List Biological Labs), …

    Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
    Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Lipopolysaccharide Derived From the Lymphoid-Resident Commensal Bacteria Alcaligenes faecalis Functions as an Effective Nasal Adjuvant to Augment IgA Antibody and Th17 Cell Responses

Wang, Y;Hosomi, K;Shimoyama, A;Yoshii, K;Nagatake, T;Fujimoto, Y;Kiyono, H;Fukase, K;Kunisawa, J;

Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

  • Immunization

    Immunization was performed as described previously (17). Briefly, on days 1, 7, and 17, the mice were intranasally immunized with 5 μg of ovalbumin (OVA) (Sigma-Aldrich) alone or 10 μg of Alcaligenes LPS or 1 μg of cholera toxin (CT) isolated from Vibrio cholerae (List Biological Laboratories, Campbell, CA, USA) in 15 µL of PBS and administered as 7.5 µL in each nostril of mice without anesthesia. One week after the final immunization, nasal wash, bronchoalveolar lavage fluid (BALF), serum, nasal passage, NALT, cervical lymphoid nodes (CLNs), and spleen were collected as previously described (17, 18) and used for analysis.

    Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Piezo1 channels restrain regulatory T cells but are dispensable for effector CD4+ T cell responses

Jairaman, A;Othy, S;Dynes, JL;Yeromin, AV;Zavala, A;Greenberg, ML;Nourse, JL;Holt, JR;Cahalan, SM;Marangoni, F;Parker, I;Pathak, MM;Cahalan, MD;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Engineered extracellular vesicles for concurrent Anti-PDL1 immunotherapy and chemotherapy

Chen, Y;Wang, L;Zheng, M;Zhu, C;Wang, G;Xia, Y;Blumenthal, E;Mao, W;Wan, Y;

Product: Tetanus Toxoid from Clostridium tetani

  • 2.13. Recall antigen assay

    Approximately 1 × 105 PBMCs per well were seeded in a 96-well tissue culture plate and cultured in 100 μl of RPMI-1640 supplemented with 10% FBS overnight at 37 °C and 5% CO2. havPD-1 EVs in various concentration were added to the cells at 50 μl per well. In control group, 50 μl of Atezolizumab or isotype control IgG1 were added. After 1-h incubation, 50 μl of 0.8 μg/ml Tetanus Toxoid (List labs, 191A) was added to wells. After 5-day culture, supernatant was collected and IFNγ was measured (R&D Systems, DIF50) according to manufacturer’s instructions.

    Product #191 – Tetanus Toxoid from Clostridium tetani

Regulation of humoral immune response by HIF-1α-dependent metabolic reprogramming of the germinal center reaction

Li, L;Feng, C;Qin, J;Li, D;Liu, M;Han, S;Zheng, B;

Product: Unspecified List Labs LPS

  • For in vitro class-switching assays, cells were stimulated with 20 ng/mL interleukin (IL)-4 (R&D Systems, Minneapolis, MN, USA) and 10 µg/mL lipopolysaccharide (LPS; List Biological Laboratories, Campbell, CA, USA) for the time periods described …

    Author did not specify which List Labs LPS product was utilized in their research.
    List Labs provides the following LPS products:

TIM-3 restrains anti-tumour immunity by regulating inflammasome activation

Dixon, KO;Tabaka, M;Schramm, MA;Xiao, S;Tang, R;Dionne, D;Anderson, AC;Rozenblatt-Rosen, O;Regev, A;Kuchroo, VK;

Product: Toxin A from Clostridium difficile

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Visualizing Sphingosine-1-Phosphate Receptor 1(S1P1) Signaling During Central Nervous System De- and Re-Myelination

Hashemi, E;Tsai, H;Yoseph, E;Moreno, M;Yeh, L;Mehta, S;Kono, M;Proia, R;Han, M;

Product: Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

  • EAE Experiments

    EAE was induced in male S1P1 -GFP signaling mice and GFP reporter mice aged 8-12 weeks. According to methods defined by Tsai et al [30, 31], mice were immunized with an emulsion containing 200 micrograms of Complete Freund’s Adjuvant (CFA), 100 micrograms of myelin oligodendrocyte glycoprotein (MOG35-55) peptide35–55, and 200 nanograms of Bordetella pertussis toxin (lot# 181236A1, List Biological Laboratories) on days zero and two. The spleen tissues were collected after eight days of immunization which marked the onset of the clinical EAE symptoms.

    Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Smek1 deficiency exacerbates experimental autoimmune encephalomyelitis by activating proinflammatory microglia and suppressing the IDO1-AhR pathway

Duan, RN;Yang, CL;Du, T;Liu, A;Wang, AR;Sun, WJ;Li, X;Li, JX;Yan, CZ;Liu, QJ;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Human iPS cells engender corneal epithelial stem cells with holoclone-forming capabilities

Watanabe, S;Hayashi, R;Sasamoto, Y;Tsujikawa, M;Ksander, BR;Frank, MH;Quantock, AJ;Frank, NY;Nishida, K;

Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae