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adding reagent for LPS detection. Experimental procedures Materials HEK293S GnTI? cells were obtained from ATCC. LPS derived from Salmonella minnesota R595 (Re) was purchased from List Biological Laboratories, Inc. (Campbell, CA). An average molecular
Recombinant Bacillus anthracis WT PA83, F427A PA83 mutant, and (D512K + K245G-R252N) bicomponent octameric variant of PA were produced as described in refs 35, 45, 78. PA63 was prepared from PA83 using furin79 or purchased from List Biological Laboratories, Inc. (Campbell, CA, USA) in the purified form. The following chemical reagents were used: KC
Human SDF1? was purchased from PeproTech; UK14304, rapamycin, BFA, monensin, nigericin, swainsonine and Exo2 were from Sigma Aldrich; PTX was from List Biological Laboratories; gallein was from Tocris Bioscience; wortmannin, AS-604850 and GSK2292767 were from ApexBio; TGX-221 and HS-173 were from Adooq Bioscience
Page 19. 19 Reagents Highly purified rough LPS from E. coli K12 strain LCD25 was from List Biological Labs. The 3H/14C labeled LPS from Salmonella enterica sv. Typhimurium PR122 (Rc) was a kind gift from Prof. Robert Munford (NIAID). The normal plasma samples
diameter: ~20 µm) inserted with a tungsten wire was filled with 0.5-2.0 µl of the mixture of A3V-RSV-mCherry and 0.5% CTB (List Labs, Campbell, CA, USA) CTB (#703, Lot#7032H, List Labs; RRID: AB_10013220), and a mouse
May 15, 2021
European Journal Of Pharmaceutics And Biopharmaceutics : Official Journal Of Arbeitsgemeinschaft Fur Pharmazeutische Verfahrenstechnik E.V
study are given in Table 1 and Fig. 1. Cholera toxin (CT) was used as a positive control adjuvant , ,  from List Biological Laboratories (Campbell, CA, USA). The endotoxin levels in the compounds were monitored
After immunization, the mice were i.p. injected with 200 ng of pertussis toxin (List Biological Laboratories) on days 0 and 2. Animals were scored for clinical signs of EAE according to the following criteria: 0, no clinical signs; 1, complete tail paralysis; 2, abnormal gait; 3, partial hindlimb paralysis; 4, complete hindlimb paralysis; 5, moribund or death, as described before (64)
on day 0. For the relapsing-remitting EAE model, SJL/J mice were subcutaneously immunized with PLP139151 (100 ?g per mouse) emulsified with CFA containing Mycobacterium tuberculosis H37 RA with the final concentration of 2 mg/ml on day 0. Immunized C57BL/6 and SJL/J mice were then i.p. administered with 200 ng pertussis toxin (List Biological Labs, Campbell, CA, USA) on day 0 and day 2
11b (101216, 1:500 for flow cytometry) and APC anti-mouse Ly-6G (127614, 1:500 for flow cytometry) were from Biolegend. Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae was purchased from List Biological Laboratories, INC. Anti-Flag affinity gel was from Sigma. pLenti-CRISPR v2 was from Addgene. First-Strand cDNA Synthesis SuperMix was purchased from TransGen Biotech. SYBR quantitative … 11b (101216, 1:500 for flow cytometry) and APC anti-mouse Ly-6G (127614, 1:500 for flow cytometry) were from Biolegend. Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae was purchased from List Biological Laboratories, INC. Anti-Flag affinity gel was from Sigma. pLenti-CRISPR v2 was from Addgene. First-Strand cDNA Synthesis SuperMix was purchased from TransGen Biotech. SYBR quantitative