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EAE was induced in 8-week-old mice by subcutaneous immunization with 150 μg MOG35–55 peptide emulsified in complete Freund adjuvant (Difco Laboratories, Detroit,
MI) per mouse, followed by administration of 200 ng pertussis toxin (List biological Laboratories, Inc., Campbell, CA) on days 0 and 2 as described. …
by glucometer, until spontaneous diabetes was diagnosed by glucose 250 mg/ dL for two consecutive measurements. DT (List Biological Laboratories; 150) was administered to NSG-DTR mice in a single, 300-?L intraperitoneal injection of 5.0 ng total DT. Control NSG-DTR mice (phosphate-buffered saline; PBS) were treated with an equal volume of 1× PBS (Sigma-Aldrich)