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4165 citations found

Effects of a single dose of orally and rectally administered misoprostol in an in vivo endotoxemia model in healthy adult horses

Kimura, S;McCoy, A;Boothe, D;Wooldridge, A;Graff, E;Hammack, S;Cresci, L;Hofmeister, E;Lascola, K;

Product: Unspecified List Labs LPS

  • Misoprostol and LPS administration

    For each treatment arm, manure was manually evacuated prior to administration of misoprostol (M-PO, M-PR) or water (CON). For per os administration (M-PO), misoprostol tablets (100 μg; Lupin Pharmaceuticals) were dissolved in 30 mL of water and administered via oral syringe. This was followed by administration of 30 mL of water through the same syringe for a total volume of 60 mL delivered. Water (60 mL) was also administered per rectum via 16-inch 8-French red-rubber catheter advanced approximately 30 cm into the rectum. For per rectum administration (M-PR), misoprostol tablets dissolved in 30 mL of water were infused via red-rubber catheter as described above, followed by infusion of 30 mL of water through the same syringe for a total volume of 60 mL delivered. Water (60 mL) was then administered orally via syringe. Horses in the CON treatment received water per os (60 mL) and per rectum (60 mL) as already described. Immediately following treatment administration (M-PR/M-PO/CON), LPS (10 µg/mL; List Biological Labs) was administered IV at 30 ng/kg diluted in 500 mL saline (0.9% NaCl) solution as a 30-minute continuous rate infusion.

    Author did not specify which List Labs LPS product was utilized in their research.
    List Labs provides the following LPS products:

C910 chemical compound inhibits the traffiking of several bacterial AB toxins with cross-protection against influenza virus

Wu, Y;Mahtal, N;Paillares, E;Swistak, L;Sagadiev, S;Acharya, M;Demeret, C;Werf, SV;Guivel-Benhassine, F;Schwartz, O;Petracchini, S;Mettouchi, A;Caramelle, L;Couvineau, P;Thai, R;Barbe, P;Keck, M;Brodin, P;Machelart, A;Sencio, V;Trottein, F;Sachse, M;Chicanne, G;Payrastre, B;Ville, F;Kreis, V;Popoff, MR;Johannes, L;Cintrat, JC;Barbier, J;Gillet, D;Lemichez, E;

Product: Shiga Toxin 1 from Escherichia coli

Therapeutic Effect of Anti-CD52 Monoclonal Antibody in Multiple Sclerosis and Its Animal Models Is Mediated via T Regulatory Cells

Kiapour, N;Wu, B;Wang, Y;Seyedsadr, M;Kapoor, S;Zhang, X;Elzoheiry, M;Kasimoglu, E;Wan, Y;Markovic-Plese, S;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

The pro-inflammatory effect of triglyceride on human CD4+ T cells and experimental autoimmune uveitis

Tan, S;Feng, X;Liu, Z;Wang, Q;Jiang, Q;Ye, X;Li, H;Su, G;Zhou, C;Wang, Y;Yang, P;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Sex dependent glio-vascular interface abnormality in the hippocampus following postnatal immune activation in mice

Ardalan, M;Chumak, T;Quist, A;Jabbari Shiadeh, SM;Mallard, AJ;Rafati, AH;Mallard, C;

Product: ULTRA PURE LPS from Escherichia coli O55:B5

  • … All animals were kept on a 12h dark-light cycle and standard housing conditions with ad libitum food access. At postnatal day 5 (P5), C57Bl/6 mice were randomly assigned to 4 experimental groups: lipopolysaccharides (LPS) (1 mg/kg, #423, List Biological Laboratories, Campbell, CA, USA)-injected mice …

    Product #423 – ULTRA PURE LPS from Escherichia coli O55:B5

Retrograde Axonal Transport of Liposomes from Peripheral Tissue to Spinal Cord and DRGs by Optimized Phospholipid and CTB Modification

Fukui, T;Tateno, H;Nakamura, T;Yamada, Y;Sato, Y;Iwasaki, N;Harashima, H;Kadoya, K;

Product: Anti-Cholera Toxin B Subunit (Goat)

  • … For immunolabeling, sections were blocked with 10% horse serum (Thermo Fisher Scientific, Waltham, MA, USA) in 0.1 M TBS, followed by the overnight incubation with primary antibody against choline acetyltransferase (ChAT, goat, 1:200, AB144P; Sigma-Aldrich, St. Louis, MO, USA) or CTB (goat, 1:500, 703; List Biological Laboratories, Inc., Campbell, NJ, USA) at 4 °C. …

    Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Indoleamine 2,3-dioxygenase 1 activation in mature cDC1 promotes tolerogenic education of inflammatory cDC2 via metabolic communication

Gargaro, M;Scalisi, G;Manni, G;Briseño, CG;Bagadia, P;Durai, V;Theisen, DJ;Kim, S;Castelli, M;Xu, CA;Zu Hörste, GM;Servillo, G;Della Fazia, MA;Mencarelli, G;Ricciuti, D;Padiglioni, E;Giacchè, N;Colliva, C;Pellicciari, R;Calvitti, M;Zelante, T;Fuchs, D;Orabona, C;Boon, L;Bessede, A;Colonna, M;Puccetti, P;Murphy, TL;Murphy, KM;Fallarino, F;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

  • Induction of EAE

    EAE was induced with 50 μg of myelin oligodendrocyte glycoprotein fragment MEVGWYRSPFSRVVHLYRNGK (MOG35–55 peptide; #crb1000205n Cambridge Research Biochemicals) mixed with incomplete Freund’s Adjuvant (#263910, BD) containing 4 mg/ml Mycobacterium tuberculosis TB H37 Ra (#231141, BD), at a ratio of 1:1 (v/v). Mice received two subcutaneous injections of 100 μl each of the MOG/CFA mix. Mice received a single intraperitoneal injection of pertussis toxin (#180, List Biological Laboratories) at a concentration of 1 ng/μL in 200 μL of PBS. A second injection of pertussis toxin at the same concentration was inoculated two days after the EAE induction. …

    Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Validating visual evoked potentials as a preclinical, quantitative biomarker for remyelination efficacy

Cordano, C;Sin, JH;Timmons, G;Yiu, HH;Stebbins, K;Guglielmetti, C;Cruz-Herranz, A;Xin, W;Lorrain, D;Chan, JR;Green, AJ;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer