Bacterial Toxin Research Citations

2.2 Drugs

… Diphtheria toxin (List Biological Laboratories) was dissolved in saline and administered at 50 mg/kg, based on previous reports …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Mice

C57BL/6 mice were either home-bred or were purchased from SamTako Ltd (South Korea). B6.SJL, Pmel Tg , Il17a Cre mice were purchased from Jackson Laboratory. Foxp3 DTR mice were kindly provided by Dr. Sin-Hyeog Im (POSTECH) with the permission of Dr. Alexander Rudensky (Memorial Sloan Kettering Cancer Center).23 R26 DTA mice and R26 YFP mice were previously described.24 25 Il17a Cre ×R26 YFP , Il17a Cre ×R26 DTA, and Il17a Cre ×R26 DTA/YFP mouse systems were created by breeding the relevant strains. CCSP Cre ×K-ras G12D mice have been described.7 Male or female mice of 8–12 weeks were used, and each experiment was conducted using age-matched/sex-matched groups. For DTR-mediated depletion experiments, 500 µg of diphtheria toxin (List Biological Laboratories) was intraperitoneally treated every other day after tumor injection in Foxp3 DTR mouse systems. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Mice

Mice used included C57BL/6J (Charles River, #027), Tlr4 KO (Jackson Laboratory, #029015), Tlr4 floxed (Jackson Laboratory, #024872), iDTR and Mcpt5-Cre + (provided by Axel Roers, University of Technology, Dresden, Germany). All mice were used between 6 and 10 weeks. For mast cell depletion in Mcpt5-Cre + x iDTR mice, 8-week old mice were given 200 ng Diphtheria Toxin (DT, Unnicked, from Corynebacterium diphtheriae, List Biological Laboratories) per mouse intravenously once a day for 5 consecutive days prior to intradermal ear immunization. Mcpt5-Cre (- x iDTR mice were used as littermate controls. Control mice were also injected with DT to control for off target effects caused by DT. To generate mast cell-specific TLR4-deficient mice, Mcpt5-Cre + mice were crossed with Tlr4-floxed mice. Cre-non-expressing animals were used as littermate controls. ) per mouse intravenously once a day for 5 consecutive days prior to intradermal ear immunization. Mcpt5-Cre (- x iDTR mice were used as littermate controls. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… For the resistance screen, … diphtheria toxin (List Labs) (Supplementary Information) …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Diptheria toxin List Biological Laboratories Cat# 150 …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Diabetes Induction

Homozygous PANIC-ATTAC mice were administered a single dose of AP20187 homodimerizer (0.08125 mg/kg) prepared according to the manufacturer’s instructions (17). Female NOD mice were surveyed twice-weekly by glucometer, until spontaneous diabetes was diagnosed by glucose >250 mg/dL for two consecutive measurements. DT (List Biological Laboratories; 150) was administered to NSG-DTR mice in a single, 300-μL intraperitoneal injection of 5.0 ng total DT. Control NSG-DTR mice (phosphate-buffered saline; PBS) were treated with an equal volume of 1× PBS (Sigma-Aldrich). All animals in a cohort (with human islets from the same human donor) were injected with DT or PBS on the same day (29).

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

2.5. Multiplex immunoassay

IgG concentrations specific to vaccine antigens PT, PRN, FHA, TT and DT were measured using an in-house multiplex bead-based immunoassay (MIA). IgG specific to FIM 2/3, which is not included in Boostrix, was also measured as a proxy for natural infection. PT, PRN and FHA were kindly provided by GlaxoSmithKline (Belgium). TT was purchased from Sigma-Aldrich. DT and FIM 2/3 were sourced from List Biological Laboratories (USA). A standard curve was generated using a 10-step 3-fold serial dilution of an in-house reference serum. Blanks and two quality control (QC) samples were included on every plate to calculate % critical variance across all assays, which fell between 5.1 and 14.6%. Assay specificity was determined using inhibition and interference assays. No cross reactivity was detected (Supplementary Figs. 1 and 2). …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
• Product #186 – Fimbriae 2/3 from B. pertussis

4-hydroxytamoxifen, diphtheria toxin, and tamoxifen treatment

… Diphtheria toxin (DT, List Biological Laboratories, 150) was dissolved in PBS. 1µg (first dose) or 613 250 ng (subsequent doses) was injected intraperitoneally for each mouse. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Materials and methods

Animals

… To induce γδ T cell depletion in TcrdGDL mice, 15 ng of diphtheria toxin (List Biological) per gram body weight was administered i.p. 24 and 48 hours prior to the initiation of the experiment. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Diphtheria toxin (DTxn, product code #150, List Biologicals) was used to assess mAb binding to native toxin. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae