Bacterial Toxin Research Citations

… Diphtheria toxin (DTxn, product code #150, List Biologicals) was used to assess mAb binding to native toxin. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Experimental groups and sensitization/challenge protocols

… Simultaneously, from week 5, considering the triweekly instillations, mice were intranasally challenged on a weekly basis with SEB (500 ng) (Product# 122; List Biological Laboratories, Inc., Campbell, CA, USA). …

… followed by ip injection of 200 ng of pertussis toxin (PTX) (List Biological Labs …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… To induce EAE, 10-week-old female C57BL/6 J mice (The Jackson Laboratory, Bar Harbor, ME) were injected with an emulsion of MOG35-55 and complete Freund’s adjuvant (CFA) (Sigma-Aldrich, St. Louis, MO), as well as 2 injections of pertussis toxin (PTX) (List Labs, Campbell, CA), spaced 2 days apart …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin List Biologicals Cat# 181 …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Induction of EAE and Visual Acuity (VA) Measurements in Mice

Before immunization, mice were housed under humidity and temperature-controlled conditions with 12-hour dark/light cycle for two weeks. Mice then underwent baseline DBSI of optic nerves. Two weeks after baseline DBSI, mice were immunized with 50 µg myelin oligodendrocyte glycoprotein (MOG)35−55 peptide emulsied (1:1) in incomplete Freund’s adjuvant (IFA), followed by intravenous Pertussis toxin (300 ng; PTX, List Laboratories, Campbell, CA, USA) injection on the day of MOG35 − 55 immunization and two days later to induce EAE (Fig. 1A). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and in vivo treatment

Immunization: EAE was induced in 8-10 week-old naïve C57/B6 or female SJL/J mice by subcutaneously injection (s.c.) over four sites in the flank with 200 µg MOG 35-55 or PLP 139-151 respectively (C S bio) in an emulsion with CFA (DF3113605 Thermo Fisher Scientific). 200 ng pertussis toxin (181 List Biological) per mouse in PBS was injected i.p. at the time of immunization and 48 h later for C57/B6 mice. Female SJL/J mice only received one injection of pertussis toxin at the time of immunization. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… All mice then received a single intraperitoneal injection of pertussis toxin (List Biological Laboratories, 180) …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

PBMC stimulation, flow cytometry and gating strategy

… Positive control samples were stimulated using Staphylococcal Enterotoxin B (SEB, List Biological Laboratories) at 1μg/mL. …

… Pertussis toxin List Biological Cat# 180 …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer