Bacterial Toxin Research Citations

… emulsified with Freund’s Complete Adjuvant (CFA) containing 5 mg/mL heat-killed Mycobacterium tuberculosis H37RA (Difco Laboratories, Detroit, MI, USA) on day 0. In addition, 200 ng of Pertussis toxin (List Biological…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.2. Reagents

… Ultrapure Lipopolysaccharide (LPS) was purified from Salmonella minnesota R595 (List Biological …

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

… A total of 50 µl emulsion was deposited on either side of the tail base. Pertussis toxin (300 ng per 200 µl; 180, List Biological Laboratories) was intraperitoneally injected on days 0 and 2 after MOG immunization …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

2.5. Multiplex immunoassay

IgG concentrations specific to vaccine antigens PT, PRN, FHA, TT and DT were measured using an in-house multiplex bead-based immunoassay (MIA). IgG specific to FIM 2/3, which is not included in Boostrix, was also measured as a proxy for natural infection. PT, PRN and FHA were kindly provided by GlaxoSmithKline (Belgium). TT was purchased from Sigma-Aldrich. DT and FIM 2/3 were sourced from List Biological Laboratories (USA). A standard curve was generated using a 10-step 3-fold serial dilution of an in-house reference serum. Blanks and two quality control (QC) samples were included on every plate to calculate % critical variance across all assays, which fell between 5.1 and 14.6%. Assay specificity was determined using inhibition and interference assays. No cross reactivity was detected (Supplementary Figs. 1 and 2). …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
• Product #186 – Fimbriae 2/3 from B. pertussis

Antigens and biotinylation

… pertussix toxin (List Biological Laboratories), …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

STAR★methods
KEY RESOURCES TABLE
REAGENT OR RESOURCE Source Identifier

… Ultra-pure LPS List Biological Laboratories Cat# 225 …

ELISA
Mouse peritoneal macrophages were primed with ultra-pure LPS (3 ng/ml; List Biological Lab) for 3 hr and were then stimulated with crystals for 3 hr at 37°C. IL-1β in cell culture supernatants (sup.) was measured by ELISA (R&D Systems).

• Product #225 – LPS from Salmonella typhimurium

Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae List Biological Laboratories, INC. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Pertussis Toxin List Biological Technologies Cat# 180 …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

4-hydroxytamoxifen, diphtheria toxin, and tamoxifen treatment

… Diphtheria toxin (DT, List Biological Laboratories, 150) was dissolved in PBS. 1µg (first dose) or 613 250 ng (subsequent doses) was injected intraperitoneally for each mouse. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Pertussis toxin (salt free; 250 ng; List Biological Laboratories, Inc.) was injected intraperitoneally on day 0 and day 2 post-immunization. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)