Bacterial Toxin Research Citations

Tetramer, antibodies, and other reagents

… For antibody blocking experiments, BC-CML-bearing mice were injected with 200 μg/mouse αPD-1 (RMP1-14, BioLegend), 200 μg/mice αLAG3 (C9B7W;64 gift of Dario Vignali and Creg Workman), 200 μg/mice αTim-3 (RMT3-23, BioLegend), 500 μg/mice αTIGIT (10A7, Genentech) or respective murine or rat isotype controls (BioXCell), i.p. three times per week for 2 weeks starting from day 7 post BMT. Diphtheria toxin (DT) was from List Biological Laboratories. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Western Blotting

… Media concentrates and cell lysates were analyzed by Western blot (Figure S2) using antibodies against C. difficile toxin A or B (List Biological Labs, Inc., Campbell, CA, USA) followed by a goat anti-chicken horseradish peroxidase secondary antibody (Invitrogen).

Mouse model of -lactoglobulin food allergy: sensitization and probiotic administration.

Our model was adapted from the work of Li et al. (38). After an adaptation period, female BALB/cByJ mice
were either sensitized once a week for 5 weeks by intragastric administration of WP (15 mg/mouse;
Lacprodan 80; Arla, Lyon, France) and CT (10 µg/mouse; List Biological, Campbell, CA) suspended in
100 µl of PBS (sensitized group) or treated with 10 µg CT alone in PBS (nonsensitized group) (Fig. 1). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

The introduction of Pertussis toxin

Ptx (List Biological Laboratories #180), was injected at 1.5μg/200μl. This was performed intra-venously 5 hours prior to live imaging by multi-photon microscopy. N = 3–5 animals per group, 33 total. Surgical set up takes about 15 minutes, and the animal is on the scope for about an hour—1 1/2 hrs resulting in 30–45 mins of usable data.

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Elisa

TcdA and TcdB toxins of the infected tree shrew stools were detected by ELISA method. The fresh fecal samples were collected after C. difficile infection for one week of all animals. Feces were suspended to 0.5 g/ml concentration by using PBS buffer (pH 7.4). The fecal suspensions were coated in 96-well plate at 4 °C overnight, and then washed with PBST (PBS pH 7.4 + 0.05% tween-20). The plate was blocked with 5% skim milk at 37 °C for 2 h. The polyclonal antibodies of TcdA and TcdB (List Biological Laboratories, Chicken IgY, 1: 1000 dilution) were used for first hybridization; then Goat anti-Chicken IgY Secondary Antibody, HRP (Invitrogen, 1: 5000 dilution) was used. ELISA-TMB Chromogenic Reagent kit (Sangon, China) was used to determine the absorbance at 450 nm. Pure toxin A and toxin B (List Biological Laboratories) were used as positive controls, and the standard curves were determined at 20 ng/ml, 2 ng/ml, 0.2 ng/ml, 0.02 ng/ml and 0.002 ng/ml both for TcdA and TcdB.

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile

Oral Sensitization and Challenge of Mice

After 2 weeks, mice were sensitized weekly for 5 times with 10 µg cholera toxin (List Biological Laboratories, Campbell, CA, USA) or 20 mg whey (sweet whey protein concentrate 60 [sWPC60];

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Immunohistochemistry

… CTB (goat, 1:4,000, List Biological Laboratories),…

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… For basophil depletion, WT and basophil-depleted mice were treated with 0.375μg of diphtheria toxin (catalog no. 150; LIST Biological Laboratories) intraperitoneally every other day; mice were killed 37?days post-N. brasiliensis infection …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Induction of EAU and clinical scoring

To induce EAU, 300 μg Inter photoreceptor binding protein (IRBP) residues 651–670 (Hanhong group, Shanghai, China) were emulsified with an equal volume of complete Freund’s adjuvant (CFA) (Sigma-Aldrich, USA) containing 2.5 mg/ml Mycobacterium tuberculosis strain 37RA (Sigma-Aldrich, USA). Pertussis toxin (0.5 μg) (List Biological Laboratories, USA) was administered intraperitoneally on the day of immunization and 24 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… The EAE model was provided by “Salari Institute of Cognitive and Behavioral Disorders (SICBD)”. …All mice received two doses of 0.3 μg pertussis toxin (List Biological Lab, Campbell, CA, USA) through theintraperitoneal (i.p.) injection about two hours and forty-eight hoursafter immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)