Bacterial Toxin Research Citations

… Bacillus anthracis protective antigen (List Biological Laboratories, cat. no. 171E) …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

2.1. Induction of EAE with Myelin Oligodendrocyte Glycoprotein (MOG)35–55 Peptide

Reagents for EAE induction were prepared by diluting MOG35–55 peptide (2 mg/mL; Cat #: AS-60130-5; AnaSpec, Fremont, CA, USA) in UltraPure™ DNase/RNase-Free distilled water (ThermoFisher, Waltham, MA, USA) and emulsifying with equal parts of Complete Freund’s Adjuvant (BD Biosciences, Franklin Lakes, NJ, USA) containing 8 mg/mL Mycobacterium tuberculosis H37RA (Cat #: 231131; BD Biosciences, San Jose, CA, USA) by passage through glass Luer-Lok syringes and a micro-emulsifying needle for 45 min. The emulsion was then transferred to 1 mL Luer-Lok syringes with 27G ½” needles. Pertussis toxin was diluted in UltraPure™ water (2 ng/μL; Cat #: 181; List Biologicals Laboratories, Campbell, CA, USA) and transferred to syringes as described above. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… For pyrin inflam- masome activation, cells were resuspended in Opti-MEM (Life Technol- ogies) and stimulated with C. difficile supernatant (AB 2 [control] or AB+) for 12-16 h. Alternately, cells were stimulated with 0.2 mg/ml TcdB (List Biological Laboratories)…

• Product #155 – Toxin B from Clostridium difficile

Induction of EAE model

… Each rat was immunized by subcutaneous injection of antigen emulsion at a dose of 0.75ml/kg into its footpad, followed by an auxiliary injection of 10ug/kg diluted in 0.5ml pertussis toxin (List Biological Laboratories) in the dorsum of the foot. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… pathogenicity and toxin production. 0.5 ml commercially purchased purified CT (LIST Biological Laboratories, 101B, …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… To label abdominal premotor neurons in the caudal ventral respiratory group (cVRG), 6 rats were microinjected with the retrograde tracer cholera toxin subunit B (CTB, List Biologicals …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Test sera were duplicated and diluted two-fold starting with a 1:50 PA 500 ng/mL in lethal factor (LF) 400 ng/mL (List Biological Laboratories Inc., USA). …

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

Induction of EAE and Clinical Assessment of Animals

Active EAE was induced according to a standard protocol [35]. Mice were immunized on day 0 by subcutaneous injection on both sides of the tail base with 100 μL of an emulsion composed of either PLP139-151 or MOG35-55 peptide in an equal volume of Freund’s adjuvant, supplemented with 4 mg/mL killed M. tuberculosis H37Ra (Difco Laboratories, Detroit, MI). The initial dose of antigen was 100 μg of PLP peptide for inducing relapsing EAE in SJL/J mice, or 200 μg of MOG peptide per GFAP-luc/B6(Cg)-Tyrc-2J/J mouse for induction of acute monophasic EAE. GFAP-luc/SJL/J mice received a second PLP antigen immunization on day 7. Immediately following these immunizations, all mice received intravenous (IV) injections of 200 ng Bordetella pertussis toxin diluted in 200-μL phosphate buffered saline (PBS) (List Biological Laboratories, Campbell, CA). The GFAP-luc/B6(Cg)-Tyrc-2J/J mice received an additional IV injection of 200 ng Bordetella pertussis toxin on postinoculation day 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… according to the manufacturer’s instructions with 300 nM recombinant light chain from BoNT/C (#625A, List Biological Laboratories). After electroporation, the keratinocytes were washed and then seeded with sensory neurons …

• Product #625A – Botulinum Neurotoxin Type C Light Chain, Recombinant

… The assay mixtures were allowed to incubate at r.t. for 30 min, before 25 μL of SNAPtide substrate flP6 (List Labs) dissolved in assay buffer was added to give a final substrate concentration of 4 μM, initiating the reaction …

• Product #523 – SNAPtide® Peptide Substrate flP6(DABCYL/5-IAF) for C. botulinum Type A Neurotoxin