Citations

Induction of concanavalin A-induced hepatitisand experimental autoimmune encephalomyelitis

For induction of experimental autoimmuneencephalomyelitis (EAE), C57BL/6 or SJL/J mice wereinjected subcutaneously with 100 µg of myelin oligodendro-cyte glycoprotein (MOG) 35–55 peptide (MEVG-WYRSPFSRVVHLYRNGK) or Myelin proteolipidprotein (PLP) 139–151 (HSLGKWLGHPDKF) emulsifiedwith complete Freund’s adjuvant on day 0 and intravenous
intravenousinjection of 400 ng of pertussis toxin (List BiologicalLaboratories, Campbell, CA, USA) on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… pertussis toxin was from List Biological Laboratories (Campbell, CA), …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… List Biological Laboratories, Inc., Campbell, CA) was injected at either 6 or 7 weeks of age at either 25 or 50 ng/g depending on the potency of DT, which varied with each lot and over time during storage at 20 C. DT was delivered intramuscularly (IM) …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

2.2. EAE induction

C57BL/6 were immunized subcutaneously with 150 μg of rodent MOG p35–55 (rpMOG) (Genemed Synthesis Inc.) or human recombinant MOG protein1-125 (hMOG) (Anaspec) emulsified in Complete Freund’s adjuvant (5 mg/ml heat killed M. tuberculosis), followed by intraperitoneal injection of 250 ng Bordetella pertussis toxin (List Biological Laboratories Inc.) at day 0 and day 2 following immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Thereafter, intranasal OVA challenge was performed 3 times a week from day 21 to day 102. In addition, mice were challenged weekly with 10 ng of staphylococcus enterotoxin B (SEB) (List Biological Laboratories, Campbell, CA) from day 49 to day …

Chemicals

… PA63 protein was prepared from PA83 using furin (53) or purchased from List Biological Laboratories (Campbell, CA) in the purified form. …

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

Induction and clinical scoring of EAE

Direct immunization against MOG35–55 in C57Bl/6J and TCR2D2 mice
Animals were immunized subcutaneously with 100 μg of MOG35–55 peptide (Genemed Synthesis, San Antonio, TX, USA) in complete Freund’s adjuvant (CFA) containing 400 μg Mycobacterium tuberculosis (Mt) H37Ra (Difco Laboratories, Detroit, MI, USA). Mice received 200 ng PT (List Biological, Campbell, CA, USA) by i.p. injection at the time of and 48 h post-immunization. Control mice were sham-immunized with phosphate-buffered saline in CFA and received the same PT dosage.

Direct immunization against PLP139–151 in SJL/J mice
SJL/J mice were injected with 100 μg PLP139–151 in 400 μg CFA subcutaneous and 2 × 50 ng PT i.p. on days 0 and 2. Control mice were sham-immunized with phosphate buffer saline in CFA and received the same PT dosage.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.2. Antigens

Vibrio cholerae toxin (CT; lot 10154B5A) and diphtheria toxoid (DPT; lot 1517B3) were from List Biological Labs (Campbell, CA, USA); …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
• Product #151 – Diphtheria Toxoid, from Corynebacterium diphtheriae

… These inoculations were followed with pertussis toxin (PTx) (List Biologicals) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Next, the cells were stimulated by adding 100 ng/ml LPS (ultrapure smooth LPS from E. coli O111:B4; List Biological Laboratories, Campbell, CA) for 1 h or 4 h. …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4