Bacterial Toxin Research Citations

… A: representative images of LifeAct-Ruby HIEs over time (116 h) after exposure to Fluorobrite DMEM medium alone or purified TcdA (List Biologicals) in Fluorobrite DMEM …

• Product #152C – Toxin A from Clostridium difficile

Depletion and monitoring of platelets

… To model thrombocytopenia by diphtheria toxin receptor-mediated megakaryocyte depletion, PF4-iCre mice were crossed to ROSA26iDTR (iDTR) mice (iDTRPf4). On the first day of depletion, 400 ng of diphtheria toxin (DT) (List Biological Laboratories, Cat. No. 150) was injected i.p. into Cre-positive mice and Cre-negative littermates. On the second and fourth days of depletion, 200 ng of DT was injected i.p. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… To identify the neurochemical nature of LT cells projecting to the LA and the AStr, single retrograde tracings were carried out using CTB (List Biological Laboratories, 104) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Experimental Autoimmune Encephalomyelitis

Experimental autoimmune encephalomyelitis (EAE) was induced as described previously (Radbruch et al., 2016). Briefly, mice were immunized subcutaneously with 250 μg of MOG35–55 (Pepceuticals, United Kingdom) emulsified in CFA (BD Difco, Germany) and received 400 ng pertussis toxin (PTx, List Biological Laboratories, Inc.) intraperitoneally at the time of immunization and 48 h later. Intravital multi-photon microscopy was carried out at the peak of disease.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of targeted EAE with DREADD

… We intravenously administered pertussis toxin (200 ng, List Biological Laboratories) immediately prior to and 48 h after cytokine injection. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE

EAE model was induced following the procedure as described previously [15,16]. … Furthermore, an intraperitoneal injection of 0.5 ml pertussis toxin (PT) (List Biological Labs, Inc, San Josa, CA) was given at the beginning of the induction and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… The mice also received intraperitoneal injections of 300 ng pertussis toxin in 100 l PBS (List Biological Lab, Campbell, CA, USA) on days 0 and 2 and were monitored for disease activity that was scored every day until day 25 …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

CT was purchased from List Biological Laboratories, Inc. (Campbell, CA, USA).

Immunization

… To remove nasal mucus, 15 μg of N-acetylcysteine (Sigma-Aldrich, St Louis, MO, USA) in 10 μl of PBS was intra-nasally administered to mice as previously described (35). After 30 min, PspA and CT were intra-nasally administered to mice as described above.

Enzyme-linked immune sorbent assay

Nunc MaxiSorp flat-bottom 96-well plates (Thermo Fisher Scientific, Waltham, MA, USA) were coated with PspA (0.05 μg per well) or CT (0.5 μg per well) in PBS and incubated overnight at 4°C. …

Enzyme-linked immunospot

Enzyme-linked immunospot (ELISpot) plates (MSHAN45, Millipore, Burlington, MA, USA) were coated with PspA (0.05 μg per well) or CT (0.5 μg per well) and incubated overnight at 4°C. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Reagents

Pertussis toxin was purchased from List Biological Labs (Campbell, CA).

EAE induction

For chronic EAE induction, female C57BL/6 mice with an age of 8–9 weeks old were injected subcutaneously with an emulsion of 200 μg MOG35–55 peptide in Complete Freund’s adjuvant containing Mycobacterium tuberculosis H37 RA (final concentration 2 mg/ml) on day 0 followed by intraperitoneal administration with 200 ng pertussis toxin on day 0 and day 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… The mice were then subsequently intraperitoneally injected with 200 ng of pertussis toxin (List Biological Laboratories) on day 0 and day 2 …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)