Bacterial Toxin Research Citations

Sensitization of mice.

Mice received 1 mg of cashew protein extract supplemented with 10 μg of Cholera Toxin (List Biological Laboratories) per intragastric route, at a rate of one intragastric administration per week for 6 consecutive weeks.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… LPS (E. coli O11: B4-derived) was purchased from List Biologicals (Campbell, CA, USA), …

• Product #201 – LPS from Escherichia coli O111:B4

… Mice also received two intraperitoneal injections of 350 ng of pertussis toxin (List biological laboratories, Inc) at the time of induction and 48 hours later …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

3.2. EAE induction

… After MOG35-55 injection, mice received varying amounts (as listed) of Bordetella pertussis toxin (PTX) in glycerol buffer depending upon the experiment,
intraperitoneally (List Biological Laboratories, Campbell, CA; provided with Hooke Kit™ for EAE Induction) on day 0 and on day 1. …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… On experimental days 0, 7, 14, 21, and 28, mice (n= 8/group) were orally sensitized, by using a blunt needle, to the hen’s egg protein OVA (20 mg/0.5 mL PBS; grade V; Sigma- Aldrich) using cholera toxin (CT; 15 µg/0.5 mL; List Biological Laboratories …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Phenotypes of toxin-receptor-gene knockouts by AncBE4max

sgRNAs targeting ANTXR1 and HBEGF were lentiviral infected into HeLa cells. GFP+ cells were FACS sorted and treated with PA/LFnDTA (70 ng/ml PA plus 50 ng/ml LFnDTA) for 48 hours or 7.5 ng/ml Diphtheria toxin (DT, List Biological Laboratories Inc.) for 60 hours and conducted in triplicates with individual treatment. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Unnicked diphtheria toxin (List Biologicals) was reconstituted at 1mg/1 mL in PBS and aliquoted and stored at -80C. Mice were injected i.p. at a dose of 40 ng/g two days prior to secondary challenge, and administered i.n. 50L (12ng/L) one day prior to secondary challenge …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Histology and microscopy

For vermal Purkinje cell retrograde tracing, goat ati-cholera toxin B subunit primary antibody (1:15000, List labs, 703) …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Immune system reactivity was supported by intraperitoneal administration of 0.6 μg or 1.2 μg PT (List Laboratories, Campbell, California, USA) dispersed in PBS (phosphate buffer saline) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Disruption of BBB and passive transfer of IgG from NMOSD patients

Animal procedures are summarized in Fig. 1a. To disrupt the BBB, mice were anaesthetized with intraperitoneal (i.p.) injection of ketamine (100 mg/kg) and xylazine (10 mg/kg). They received subcutaneous injections of complete Freund’s adjuvant (CFA, BD Biosciences, USA) containing heat-killed H37Ra Mycobacterium tuberculosis (Difco, USA) at 4 sites (50 μg in 50 μl CFA each site) on the hind flank on 7 days before IgG transfer. In addition, mice received i.p. injections of pertussis toxin (PTx, 200 ng in 0.2 ml PBS, List Biological Laboratories, USA) on 7 and 3 days prior to IgG transfer.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)