Bacterial Toxin Research Citations

EAE immunization:

… EAE was induced by subcutaneous injection of 300g of myelin oligodendrocyte glycoprotein peptide (MOG3555; KJ Ross-Petersen ApS, Copenhagen, Denmark) emulsified with complete Freund’s adjuvant (CFA; Sigma, USA) in both hind flanks of each mouse. All mice received an intraperitoneal (i.p.) injection of 0.3µg pertussis toxin (List Biological Lab, USA) immediately and 48h after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Macrophage isolation and polarization:

Using aseptic technique, femurs were harvested, and the bone marrow flushed from C57BL/6 and Tmem173^gt mice for macrophage polarization studies. The BMDMs were expanded in culture for 7 d in the presence of L-929 mouse fibroblast conditioned medium as a source of M-CSF. Cells were re-seeded onto 6-well plates at 210⁶ cells/well and polarized for 48 hrs with the addition of 50ng/ml LPS (List Biological Labs) and 50ng/ml murine IFN (Cedarlane) for M1, 40ng/ml murine IL-4 (R&D Systems) for M2 or left untreated for M0 cells. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… After 7 days, BMDMs were treated with nHDL in RPMI-1600 media containing 2% FBS for 18 hours, followed by washing of cells and LPS stimulation (ultrapure LPS; 10 ng/mL; List Biological Laboratories, Inc; NC9633766) for 4 hours in the presence of 30% L929-conditioned …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… For Treg ablation, Foxp3-DEREG mice were injected intraperitoneally with 1.5 g (75 g/kg) diphtheria toxin (List Biologicals) at days 1 and 2 post antigen immunization. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Samples were run in parallel for phenotypic analysis (above) and intracellular cytokine staining. 1-1.5 million PBMC suspensions were cultured at a final volume of 200µl of complete RPMI medium in the presence of Staphylococcus Aureus Enterotoxin B (SEB, List Biological Labs, CA) …

… Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae List Biological Laboratories Cat# 150 …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… LPS-neutralizing assays utilized smooth-type LPS from Escherichia. coli O55:B5 (List Biological Laboratories, Campbell, CA, USA) as an endotoxin. …

• Product #203A – LPS from Escherichia coli O55:B5

Chemicals and Reagents:

… Recombinant LF was from List Biological Laboratories (Campbell, California). …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169L (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

Mouse Primary Corneal Epithelial Cells (MPCEC):

… Primary corneal epithelial cell cultures were established using a modification of the established explant culture method.^38,39 Briefly, eyes were enucleated, and the cornea was washed with Ca⁺⁺-free PBS (pH 7.2). Each cornea was cut in half and placed in a 35-mm dish (Fisher Scientific) with the epithelial side up. One and half milliliters of DMEM with 10% serum containing 40 g/mL gentamicin, 1% ITS, and 100 ng/mL cholera toxin (LIST Biological Laboratories, Inc., Campbell, CA, USA) was added and the tissue was cultured in a humidified incubator at 37C with 5% CO2. Culture medium was replaced every 2 days. Cells were passged using 0.25% trypsin (Fisher Scientific), and subculturing in DMEM with 3% serum containing 40 g/mL gentamicin, 1% ITS, and 100 ng/mL cholera toxin. 

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Cells and Reagents:

… Salmonella minnesota ultra pure lipopolysaccharide (LPS) was from List Biologicals Laboratories (Campbell, CA), …

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)