Bacterial Toxin Research Citations

… RGCs were labeled with 1% cholera toxin B subunit (CTB; List Biological Laboratories, Campbell, CA) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… The retrograde tracers CTB (0.5% diluted in distilled water; List Biological) or fluorogold (FG; 2% in 0.1M cacodylate buffer; Fluorochrome) were iontophoretically injected (for CTB, 7/7s on/off duty cycle, 1A; for FG, 2/2s on/off duty cycle, 1 …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Vero cell cytotoxicity assay of C. difficile positive fecal samples

… Control containing purified C. difficile toxins (A and B; List Biological Labs, CA, USA) and antitoxin were included. …

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile
• Product #753A – Anti-Clostridium difficile Toxin A (Chicken lgY)
• Product #754A – Anti-Clostridium difficile Toxin B (Chicken lgY)

IL-12p40 flow cytometry assays:

For determination of IL-12p40 production by activated DCs andmacrophages, GM-CSF cultures were set up from get40 mice as de-scribed in section 2.2.1. On day 9, cells were stored at 4 C for 30 minand afterwards scraped off the dishes. Cells were centrifuged, re-suspended in fresh medium and counted for culturing in non-treated12-well plates (Sigma-Aldrich). Cells were seeded at a density of5 105cells/well for a final volume of 1 ml and initially treated with 5concentrations of 01 M CpG 2216 (TIB Molbiol) or 010 ng/ml lipopolysaccharide (LPS; List Biological Laboratories, Inc.), respectively,for 16 h to determine suboptimal stimulation conditions. For testing ofnatural compounds, cells were incubated with 0.1, 1 or 10 M of nat-ural products with or without 0.1 M CpG 2216 or 1 ng/ml LPS or leftuntreated at 37 C for 16 h. Untreated cells or cells treated with 0.1 MCpG 2216 or 1 ng/ml LPS were used as controls. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… Endotoxins from Escherichia coli O55:B5, E. coli O111:B4, Salmonella minnesota R595 Re, S. typhimurium were purchased from List Biological Laboratory, Inc. (CA, USA). …

• Product #203A – LPS from Escherichia coli O55:B5
• Product #304 – LPS from Salmonella minnesota R595 (Re)
• Product #225 – LPS from Salmonella typhimurium

Stereotactic surgery (6-OHDA infusion and BoNT-A Injection):

… 6-OHDA infused and control rats then received BoNT-A injection (List Biological Laboratories, Campbell, California, USA) at the right EPN four weeks after 6-OHDA surgery. Delivery of 0.5 ng of BoNT-A into the EPN was performed by injection of 0.5 uL of BoNT-A solution (0.5 L of 1 ng/uL BoNT-A dissolved in phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)). Infusions were performed over 5 minutes via a 31G injection cannula attached to a 10 uL Hamilton syringe. Correspondingly, vehicle animal groups were administered with an equivalent volume of PBS with 0.1% BSA. The stereotactic coordinates with reference to bregma were: AP -2.5 mm, L 3 mm, DV 7.7 mm (Paxinos and Watson, 2009).

EAE immunization:

… EAE was induced by subcutaneous injection of 300g of myelin oligodendrocyte glycoprotein peptide (MOG3555; KJ Ross-Petersen ApS, Copenhagen, Denmark) emulsified with complete Freund’s adjuvant (CFA; Sigma, USA) in both hind flanks of each mouse. All mice received an intraperitoneal (i.p.) injection of 0.3µg pertussis toxin (List Biological Lab, USA) immediately and 48h after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Macrophage isolation and polarization:

Using aseptic technique, femurs were harvested, and the bone marrow flushed from C57BL/6 and Tmem173^gt mice for macrophage polarization studies. The BMDMs were expanded in culture for 7 d in the presence of L-929 mouse fibroblast conditioned medium as a source of M-CSF. Cells were re-seeded onto 6-well plates at 210⁶ cells/well and polarized for 48 hrs with the addition of 50ng/ml LPS (List Biological Labs) and 50ng/ml murine IFN (Cedarlane) for M1, 40ng/ml murine IL-4 (R&D Systems) for M2 or left untreated for M0 cells. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… After 7 days, BMDMs were treated with nHDL in RPMI-1600 media containing 2% FBS for 18 hours, followed by washing of cells and LPS stimulation (ultrapure LPS; 10 ng/mL; List Biological Laboratories, Inc; NC9633766) for 4 hours in the presence of 30% L929-conditioned …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… For Treg ablation, Foxp3-DEREG mice were injected intraperitoneally with 1.5 g (75 g/kg) diphtheria toxin (List Biologicals) at days 1 and 2 post antigen immunization. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae