Bacterial Toxin Research Citations

Reagents

… Lethal Toxin was from List Biological Laboratories. …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

• Product #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

Synthetic CII peptide, Toll-like receptor (TLR) ligands, and reagents

… Ultra-pure lipopolysaccharide (LPS; from Escherichia coli 0111:B4) was purchased from List biological laboratories, Inc. (Campbell, CA). …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

… The introduction of Pertussis toxin: Ptx (List Biological Laboratories #180), was injected at 1.5µg/200µl. This was performed intra-venously 5 hours prior to live imaging by multi-photon microscopy.

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… Botulinum neurotoxin A, Clostridium botulinum List Biological Labs Cat# 130B …

• Product #130 – Botulinum Neurotoxin Type A from Clostridium botulinum

Animals and treatment

On the 14th day before their sacrifice, mice were induced with LPS (LPS Escherichia coli O111: B4, List Biological Laboratory, Inc.) by administering as much as 10 L of 4 mg/ml LPS solution intranasally.

• Product #201 – LPS from Escherichia coli O111:B4

Induction of EAE in mice

EAE mice were induced with MOG35-55 (200 µg), and the mice were intraperitoneally injected with pertussis toxin (500 ng, List Biological Laboratories, Inc.) at 0 and 48 h following immunization. At least two investigators weighed and evaluated the animals for clinical scores in a blinded manner. There were two animals which appeared to be in intolerable distress and self-mutilated limbs; these were sacrificed using pentobarbital sodium (150 mg/kg).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… One hundred nanograms of pertussis toxin (List Biological Labs, Campbell, CA, USA) in 0.1 mL saline was also injected intraperitoneally on day 0, and 48 hours later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Antibody measurements:

For PT-specific IgG ELISA, plates were coated with 0.5µg/mL purified PT (List Biologicals Campbell, California) in PBS and the assay performed as previously described [133]. …

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

… The effect of Ex-4 on cytokine levels in microglia cell cultures was also studied. Whole brains of young mice (day 2-3 after birth) were homogenized and microglia cells cultured and enriched. They were then stimulated with lipopolysaccharide (LPS) (10 ng/mL, List Biological Laboratories Inc., Cambell, CA) with or without Ex-4 (10 nmol/L). …

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/

… During the final 8 weeks, mice were challenged with 10 ng of Staphylococcal enterotoxin B (SEB; Product# 122; List Biological Laboratories Inc., Campbell, CA, USA), diluted in 1 PBS, immediately after the instillation of 3% OVA. …