Bacterial Toxin Research Citations

Induction and assessment of EAE:

For active EAE induction, age- and sex-matched mice were immunized s.c. with MOG_35-55 peptide (300 µg) mixed in CFA (Sigma-Aldrich) containing 5 mg/ml heat-killed Mycobacterium tuberculosis H37Ra (Difco). Pertussis toxin (200 ng, List Biological Laboratories) in PBS was administered i.v. on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Muscle injury model:

… One hour after the first immunisation, pertussis toxin (500ng in 100l saline; List Biological Laboratories, Campbell, CA, USA) was intraperitoneally injected into each animal. 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Retrograde tracer injections:

Injections of cholera toxin beta sub-unit (CTb; List Biological Laboratories, Campbell, CA) were used to retrogradely label premotor cells with axonal projections to the XII motor nucleus, …

Author did not specify which CTB was utilized.  List Labs has provided Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae) and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).  The former has been discontinued.  The latter may be substituted for the former, if the researcher takes into account the difference in the buffer in which each product is provided and the amount of protein per vial.  When Product #103B was reconstituted with 200 l of water, it contained 1 mg of protein in 0.05 M Tris, 0.2 M NaCl, 0.001 M Na₂EDTA, 0.003 M NaN_3, pH 7.5.  When Product #104 is reconstituted with 0.25 ml water, it contains 0.5 mg of Cholera Toxin B Subunit (choleragenoid) in 0.01 M Sodium Phosphate at pH 7.5.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Mass spectrometry:

Four-week old mice were euthanized, globes were exenterated and sterilized in 2% iodine (Sigma-Aldrich, St Louis, MI), after which the limbus was dissected, placed epithelium-side-down in 6-well culture plates (Corning Inc., Corning, NY), and incubated in defined keratinocyte serum-free media (dKSFM; Gibco, Fremont, CA) containing 50nM recombinant human epidermal growth factor (Peprotech, Rocky Hill, NJ), 500nM Cholera Toxin (List Biological Labs Inc., Campbell, CA) and 100 U/ml Penicillin-Streptomycin (Thermo Fisher Scientific, Waltham, MA) at 37C in 5% CO_{2. …}

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… CTb Gt Polyclonal List Biological Labs 102946-502 7032A10 1:5,0001:10,000 …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Pertussis toxin (300 ng/200 µL; 180, List Biological Laboratories) was injected intraperitoneally (ip) on days 0 and 2 after MOG immunization. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

EAE:

… For active immunization mice were immunized subcutaneously into the flanks with 200 µg MOG35-55 peptide (Anawa) emulsified in complete Freunds adjuvant (CFA; InvivoGen) and 200 ng pertussis toxin (List Biological Laboratories Inc.) intraperitoneally on day 0 and day 2 post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Pertussis toxin (200 ng; List Biological Laboratories, Campbell, CA, USA) was intraperitoneally administrated at 0 and 48hr postimmunization. EAE scores were assessed daily …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… For adoptive transfer of effector T cells, 5×106 6 Th17 or Th1 1C6 T cells 7 were injected i.p., in cold PBS (Cellgro) into NOD.Scid recipient mice, which additionally received 200 ng of pertussis toxin (List Biological Labs) i.p. on d0 and d2 post-injection. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Cells were incubated for 30 minutes at 37°C under normal cell culturing conditions, after which 10 ng/mL of pertussis AB5 holotoxin(List Biological Laboratories Inc., 179A) was added to the cells and incubation was continued for 2 h. …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)