Bacterial Toxin Research Citations

… For retrograde tracing we injected either Fast Blue (FB; Polysciences), Fluorogold (FG; Santa Cruz Biotechnology, Heidelberg, 37 Germany), or cholera-toxin b (CtB; List Biological Labs Ltd., California, USA). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Materials:

… Toxin A (#152C) and B (#155A) from C. difficile were purchased from List Biological Labs. …

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile

… Following the FISH protocol, sections were blocked for 10min in 10% donkey normal serum (DNS) in phosphate-buffered saline (PBS) solution supplemented with 0.2% Tween-20 (PBST), then incubated for 1h with goat anti-CTb (1:2,000; List Biological Laboratories, no. 703, lot no. 7032A10). …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Pertussis toxin (200ng) (List Biological Laboratories Inc., Campbell, CA) was injected intraperitoneally on the day of and 48h following immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… The peptide was emulsified in 0.2ml complete Freunds adjuvant (CFA, Sigma). Four hours later, the mice were injected intraperitoneally with 200ng pertussis toxin (List Biological Labs), and an additional 200ng pertussis toxin 24h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… A set of CFA-only administered mice were used as control for EAE induction. Two intraperitoneal injections of pertussis toxin, Bordatella pertussis, (List Biological Labs) were also administered to all mice on the day of induction and 48 hours thereafter. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin (PTX, List Biological Laboratories, CA, USA) was injected intraperitoneally on the day of immunization and again after 2 days. MyR-c(MyD 44) was administered by gavage vs equal volume DMSO/PBS on days 0, 2, and 4. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Lipopolysaccharide (LPS, Escherichia coli 055:B5) was purchased from List Biological Laboratories, Inc. (Campbell, CA, USA) and reconstituted with phosphate-buffered saline (PBS) buffer (10 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl, pH 7.4) prior to use. …

• Product #203A – LPS from Escherichia coli O55:B5

… Cells were placed into 6-well plates for stimulation with 759 10ng/ml Salmonella Minnesota R595 (Re) ultra-pure LPS (List Biological) for different time760 points …

• Product #434 – ULTRA PURE LPS from Salmonella minnesotaR595 (Re)

Animals:

Animals were maintained in a pathogen-free environment on a 12-hour light/12-hour dark cycle with full access to food and water. SftpcCreERT2mice (59), obtained from Harold Chapman (UCSF, San Francisco, California, USA), were crossed to Rosa26-mTmG (abbreviated mTmG) mice (The Jackson Laboratory). Male SftpcCreERT2^+/;mTmG^+/ mice were given 20 mg/ml tamoxifen in corn oil at a dose of 0.25 mg/g body weight i.p. every other day for 3 doses at age 6 weeks. Recombination efficiency is shown in Supplemental Figure 1. At 12 weeks of age, mice were treated with 40 g LPS (E. coli 0111:B4, List Biological Laboratories) in 50 l 0.9% saline by intratracheal instillation or left untreated. Mice were randomly assigned to treatment groups. Mice were euthanized 7 or 27 days later. Sprague-Dawley rats aged 24 months were purchased from Charles River or obtained from the University of Michigan Rodent Recycling Program. Tgfbr2^fl/fl mice were purchased from The Jackson Laboratory.

• Product #201 – LPS from Escherichia coli O111:B4