Bacterial Toxin Research Citations

August 13, 2024

Communications Chemistry

Martínez-Carranza, M;kerlová, J;Lee, PG;Zhang, J;Kr?, A;Sirohiwal, A;Burgin, D;Elliott, M;Philippe, J;Donald, S;Hornby, F;Henriksson, L;Masuyer, G;Kaila, VRI;Beard, M;Dong, M;Stenmark, P;

Product: Botulinum Neurotoxin Type A from Clostridium botulinum

% were discounted). The preparations were then washed 3× for 5?min and after a further stabilization period of 20?min, 1?ml of 10x BoNT (final concentration 10?pM for BoNT/A and BoNT/B (both LIST Biological Laboratories, Campbell, USA), and recombinant (r)BoNT/X, and 100 pM for LHN/X, in KHB supplemented with 0.05% (w/v) gelatin type A (Sigma) was added to the tissue bath and electrical s ... % were discounted). The preparations were then washed 3× for 5?min and after a further stabilization period of 20?min, 1?ml of 10x BoNT (final concentration 10?pM for BoNT/A and BoNT/B (both LIST Biological Laboratories, Campbell, USA), and recombinant (r)BoNT/X, and 100 pM for LHN/X, in KHB supplemented with 0.05% (w/v) gelatin type A (Sigma) was added to the tissue bath and electrical s

August 13, 2024

Proceedings Of The National Academy Of Sciences

Diercks, A;Podolskaia, I;Murray, T;Jahn, A;Mai, D;Liu, D;Amon, L;Nakagawa, Y;Shimano, H;Aderem, A;Gold, E;

Product: LIPID A monophosphoryl from Salmonella minnesota R595

ions.Frozen stocks of LPS (List Biological #R595) or PAM3 (Invivogen #vac-pms) were thawed and sonicated for 5 min before dilution in BMDM medium. The diluted stimuli were again sonicated for 5 min before addition to BMDM cultures for a final concentration of 10 ng/mL for LPS and 300 ng/mL for PAM3. CIM cells were stimulated with 100 ng/mL LPS. Unless otherwise indicated, cells were incubated with 25HC (5 ?M) (Sigma #H1015), OSW-1 (0.1 nM) (Cayman Chemicals #30310), itraconazole

August 11, 2024

Journal Of Neuroinflammation

Chumak, T;Jullienne, A;Ek, CJ;Ardalan, M;Svedin, P;Quan, R;Salehi, A;Salari, S;Obenaus, A;Vexler, ZS;Mallard, C;

Product: ULTRA PURE LPS from Escherichia coli O55:B5

ious publication [14]. The day of birth was defined as postnatal day (P) 0. At P9, pups received a single intraperitoneal injection of lipopolysaccharide (LPS from E. coli O55:B5, 1 mg/kg, Cat# 423, List labs) or equivalent volume of saline. The dams remained on the experimental diet until offspring sacrifice (P10-P12). To allocate pups to particular experimental groups (LPS or saline), randomiza

August 10, 2024

Medrxiv : The Preprint Server For Health Sciences

Ismahil, MA;Zhou, G;Gao, M;Bansal, SS;Patel, B;Limdi, N;Xie, M;Antipenko, S;Rokosh, G;Hamid, T;Prabhu, SD;

Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

netic depletion was performed in CD169DTR/+ mice upon i.p. injection with 10 ?g/kg DT (List Biological, Lot #15043A1) in 100 ?L PBS. For pharmacological depletion, mice were injected i.v. with 4 mg/kg of clodronate-loaded liposomes (Clo-Lip) in 100 ?L suspension solution (Encapsula Nanosciences). This low dose of clo-lip selectively depletes macrophage

August 9, 2024

Microbes And Infection

Belcher, T;Coutte, L;Debrie, AS;Sencio, V;Trottein, F;Locht, C;Cauchi, S;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

pertussis and/or given 100 ng purified PT (List Laboratories) in 20 ?l of phosphate-buffered saline (PBS) twice 4 weeks apart. To determine the bacterial load at different ;- Bacterial-viral co-infections are frequent, but their reciprocal effects are not well understood. Here, we examined the effect Bordetella pertussis infection and the role of

August 7, 2024

Biorxiv : The Preprint Server For Biology

Willemsen, L;Lee, J;Shinde, P;Soldevila, F;Aoki, M;Orfield, S;Kojima, M;Ricardo, DSA;Sette, A;Peters, B;

Product: Tetanus Toxin from Clostridium tetani

rforming an indirect serological assay with xMAP Microspheres (details described in xMAP Cookbook, Luminex 5th edition). Pertussis, Tetanus, and Diphtheria antigens (PT, PRN, Fim2/3, TT, DT (all from List Biological Laboratories), and FHA (MilliporeSigma)) and as a negative control Ovalbumin (OVA, MilliporeSigma) were coupled to uniquely coded beads (xMAP MagPlex Microspheres, Luminex Corporation) ... rforming an indirect serological assay with xMAP Microspheres (details described in xMAP Cookbook, Luminex 5th edition). Pertussis, Tetanus, and Diphtheria antigens (PT, PRN, Fim2/3, TT, DT (all from List Biological Laboratories), and FHA (MilliporeSigma)) and as a negative control Ovalbumin (OVA, MilliporeSigma) were coupled to uniquely coded beads (xMAP MagPlex Microspheres, Luminex Corporation)

August 5, 2024

Research Square

Yu, R;Lozinski, B;Seifert, A;Ta, K;Zandee, S;Kaushik, D;Park, J;Klement, W;Larouche, S;Tsimikas, S;Witztum, J;Prat, A;Dong, Y;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

ctivated Mycobacterium tuberculosis H37Ra (Sigma-Aldrich), in which 50 µl emulsion was deposited on each side of the tail base. Intraperitoneal injection of pertussis toxin (300 ng per 200 µl; 180, List Biological Laboratories) was performed days 0 and 2 after MOG immunization. Mice were monitored and scored daily on a scale of 0-15. EAE mice during chronic disease (day 45) were euthanized wit ... ctivated Mycobacterium tuberculosis H37Ra (Sigma-Aldrich), in which 50 µl emulsion was deposited on each side of the tail base. Intraperitoneal injection of pertussis toxin (300 ng per 200 µl; 180, List Biological Laboratories) was performed days 0 and 2 after MOG immunization. Mice were monitored and scored daily on a scale of 0-15. EAE mice during chronic disease (day 45) were euthanized wit

August 5, 2024

Journal Of Advanced Research

Zhang, C;Liu, X;Gu, C;Su, Y;Lv, J;Liu, Y;Gao, Y;Chen, H;Xu, N;Xiao, J;Xu, Z;Su, W;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

vol On day 0, we injected mice subcutaneously with 200 ?g of this mixture. After immunization, 0.25 ?g of pertussis toxin (PTX) (List Biological Laboratories, Campbell, California, USA) dissolved in phosphate-buffered saline (PBS) was injected intraperitoneally on day 0 and day 2

August 4, 2024

Nature Communications

Ma, J;O'Malley, JJ;Kreiker, M;Leng, Y;Khan, I;Kindel, M;Penzo, MA;

Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

er Scientific) were injected into the TRN (0.5?µL) and PVT (0.6?µL), respectively, and allowed 4 d for retrograde transport. For retrograde tracing of PVT-projecting TRN cells unconjugated CTB (List Labs Product No. 104) was injected into the PVT (0.6?µL). For retrograde labeling of PVT-projecting TRN cells for RNAscope, retrobeads (0.6?µL, LumaFluor, Inc.) were injected into the PVT a

August 1, 2024

Experimental & Molecular Medicine

Kang, YJ;Song, W;Lee, SJ;Choi, SA;Chae, S;Yoon, BR;Kim, HY;Lee, JH;Kim, C;Cho, JY;Kim, HJ;Lee, WW;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

lsified in CFA (BD Biosciences, San Jose, CA), followed by i.p. injection of 250?ng pertussis toxin (PTX) (List Biological Labs, Campbell, CA) 4 and 24?h later. The clinical scores of EAE mice were assessed daily

Bacterial Toxin Research Citations

Activity of botulinum neurotoxin X and its structure when shielded by a non-toxic non-hemagglutinin protein

Oxysterol binding protein regulates the resolution of TLR-induced cytokine production in macrophages

Maternal n-3 enriched diet reprograms the offspring neurovascular transcriptome and blunts inflammation induced by endotoxin in the neonate

Splenic CD169 + Tim4 + Marginal Metallophilic Macrophages Are Essential for Wound Healing After Myocardial Infarction

Pertussis toxin-dependent and -independent protection by Bordetella pertussis against influenza

Th1 polarization in Bordetella pertussis vaccine responses is maintained through a positive feedback loop

Oxidized phosphatidylcholines induce chronic neurodegeneration partly through IL-1? mediated positive feedback

Histone deacetylases facilitate Th17-cell differentiation and pathogenicity in autoimmune uveitis via CDK6/ID2 axis

Convergent direct and indirect cortical streams shape avoidance decisions in mice via the midline thalamus

Inhibition of BCAT1-mediated cytosolic leucine metabolism regulates Th17 responses via the mTORC1-HIF1? pathway