Citations

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4165 total record number 127 records this year

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4165 citations found

Mitofusin 2 confers the suppression of microglial activation by cannabidiol: Insights from in vitro and in vivo models

Li, M;Xu, B;Li, X;Li, Y;Qiu, S;Chen, K;Liu, Z;Ding, Y;Wang, H;Xu, J;Wang, H;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Genetic tracing reveals transcription factor Foxp3-dependent and Foxp3-independent functionality of peripherally induced Treg cells

van der Veeken, J;Campbell, C;Pritykin, Y;Schizas, M;Verter, J;Hu, W;Wang, ZM;Matheis, F;Mucida, D;Charbonnier, LM;Chatila, TA;Rudensky, AY;

Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Insights gained from Single-Cell analysis of immune cells on Cyclosporine A treatment in autoimmune uveitis

Duan, R;Xie, L;Li, H;Wang, R;Liu, X;Tao, T;Yang, S;Gao, Y;Lin, X;Su, W;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Identification of ADS024, a newly characterized strain of Bacillus velezensis with direct Clostridiodes difficile killing and toxin degradation bio-activities

O'Donnell, MM;Hegarty, JW;Healy, B;Schulz, S;Walsh, CJ;Hill, C;Ross, RP;Rea, MC;Farquhar, R;Chesnel, L;

Product: Toxin B from Clostridium difficile

  • Toxin degradation assay

    Toxin degradation was assessed using ADS024 CFS and reconstituted lyophilized cells. The CFS was prepared as previously described and was serially diluted 1:2 in BHI broth 10 times. Lyophilized ADS024 was reconstituted and serially diluted in phosphate-buffered saline. Purified C. difficile toxins A and B (0.2 mg/mL) were obtained from List Biological Laboratories (Campbell, CA, USA). Purified toxin was reconstituted in sterile water to contain 50 mM Tris, 50 mM NaCl, pH 7.5, and 0.1% trehalose and was stored at 2–8 °C; 2.5-μL (0.2 mg/mL) aliquots of purified C. difficile toxin A or B were co-incubated for 2 h at 37 °C with 30 μL of either ADS024 CFS, or lyophilized ADS024, and the reactions were stopped, heated, and electrophoresed in a 3–8% NuPAGE Tris–acetate protein gel as 15-μL volumes/well. The gel was electrophoresed at 150 V for 50 min. Western blot using TcdA or TcdB primary antibody (1:5000 dilution from List Biological Laboratories) and fluorescent secondary antibodies was performed.

    Product #152C – Toxin A from Clostridium difficile
    Product #155 – Toxin B from Clostridium difficile

CEST MRI and MALDI imaging reveal metabolic alterations in the cervical lymph nodes of EAE mice

Thomas, AM;Yang, E;Smith, MD;Chu, C;Calabresi, PA;Glunde, K;van Zijl, PCM;Bulte, JWM;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

  • EAE induction

    All animal studies are approved by the Johns Hopkins University Animal Care and Use Committee. Animals received standard husbandry. C57Bl/6 mice (female, 6–10 weeks, Jackson Laboratories, n = 5–8) were injected s.c. with 200 μl of emulsion containing incomplete Freund’s adjuvant (Sigma F5506) Mycobacterium tuberculosis H37Ra (Difco BD 231141, 5 mg/ml) and myelin oligodendrocyte glycopeptide (MOG35-55, Johns Hopkins Synthesis and Sequencing Facility, 0.5 mg/ml). Mice were injected i.p. with 100 μl of pertussis toxin (List Laboratories #180, 300 ng) on the day of induction and 2 days later. …

    Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Visualizing Spatial and Stoichiometric Barriers to Bispecific T-Cell Engager Efficacy

You, R;Artichoker, J;Ray, A;Gonzalez Velozo, H;Rock, DA;Conner, KP;Krummel, MF;

Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Macrophages disseminate pathogen associated molecular patterns through the direct extracellular release of the soluble content of their phagolysosomes

Greene, CJ;Nguyen, JA;Cheung, SM;Arnold, CR;Balce, DR;Wang, YT;Soderholm, A;McKenna, N;Aggarwal, D;Campden, RI;Ewanchuk, BW;Virgin, HW;Yates, RM;

Product: ULTRA PURE LPS from Salmonella minnesota R595 (Re)

  • … For imaging, cells were seeded in 96 well μClear® plates (Greiner Bio-One) or NuncTM MicroWellTM optical bottom 96-well plates (Thermo Fisher Scientific) and allowed to establish a confluent monolayer 16–24 h prior to evaluation by high content or scanning confocal microscopy, respectively. For immunofluorescence analysis, cells were seeded on a 12-mm microscope cover glass (VWR) in a 24-well plate overnight. Where indicated, BMMØs were cultured in the presence of either 10 ng/mL recombinant murine IL-4 or 100 U/mL recombinant murine IFN-γ (PeproTech) for 24 and 18 h respectively, prior to phagosomal assessment. Where indicated, cells were pulsed overnight with 2 mg/mL Bella Fluor (BF) 350SE-labelled (Setareh Biotech) dextran (70 kDa) with the dextran being chased into lysosomes the following day for 2 h. Where indicated, 2.5 mM of LLOMe was added to cells 30 min after phagocytosis of experimental particles and incubated for 2 h prior to imaging. Where indicated, 10 nM of myricetin (Alfa Aesar), 10 nM of kaempherol (Selleckchem) or 100 nM of EACC (Life Chemicals) were added to cells at the same time as experimental particles. Where indicated 0.5 μM Torin 1 (Tocris Bioscience), 0.5 μM rapamycin (Sigma Aldrich), 1× MEM amino acid solution (Sigma Aldrich), 10 ng/mL ultrapure lipopolysaccharide from S. Minnesota R595 (LPS; List Biologicals) were added to assay medium 30 min after the addition of reporter particles or 50 nM Lysotracker® Red (Invitrogen) 2 h after the addition of experimental particles and remained in the assay medium for the duration of phagosomal assessment.

    Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

Development of a TLR7/8 agonist adjuvant formulation to overcome early life hyporesponsiveness to DTaP vaccination

Dowling, D;Barman, S;Smith, A;Borriello, F;Chaney, D;Brightman, S;Malhem, G;Brook, B;Menon, M;Soni, D;Schüller, S;Siram, K;Nanishi, E;Bazin, H;Burkhart, D;Levy, O;Evans, J;

Product: Pertactin from B. pertussis (69 kDa Protein)

  • … For anti-filamentous hemagglutinin (FHA) or anti-pertactin (PRN) ELISAs, CoStar 96 well high-binding
    plates (Corning, Corning, NY) were coated with either 2 µg/ml FHA (BP-FHA-100, The Native Antigen
    Company/Cedarlane, Burlington, NC) or 1 µg/ml PRN (B. pertussis Pertactin, Strain: B. pertussis strain
    165, The List Labs
    , St Campbell, CA) in carbonate buffer pH 9.6, incubated overnight at 4°C, washed 3x
    with wash buffer (KPL 10X Phosphate Buffered Saline with 0.05% Tween 20 (Fisher Scientific)) and
    blocked with Superblock (ScyTek) for 1 h at room temperature (RT). …

    Product #187 – Pertactin from B. pertussis (69 kDa Protein)

Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae