Bacterial Toxin Research Citations

… current pulses, 7 s duration every 7 s for 10 min) of the retrograde tracer cholera toxin subunit b – CTb (low salt, 1% in distilled water, List Biological Laboratories, Campbell, USA). CTb was detected using a goat anti-CTb antibody (1:2000, List Biological …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Perfusions and tissue sectioning:

… Freefloating sections were immunostained for CTb, NeuN or cFos by overnight incubation in goat antiCTb (List Biological Laboratories, 7032A9, 1: 50,000 dilution), …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… The mice were also injected with intravenous Bordetella pertussis toxin (200 ng; PTX, List Biological Laboratories Inc.) in 1X Phosphate Buffered Saline (PBS) at the time of and two days following immunization …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… For the detection of GM1 ganglioside, biotinylated cholera toxin B subunit (1:1000; List Biological Laboratories, Inc., Campbell, CA, USA) was used. …

• Product #112 – Cholera Toxin B Subunit Biotin Conjugate

… The primary antibodies used were guinea pig anti-oxytocin 1:5,000 (OXT; Peninsula Laboratories, San Carlos, CA, USA) and goat anti-CTB 1:100,000 (List Biological Labs, Campbell, CA, USA). …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Injection site visualization:

… In the other cases (S4-5, S11-13 and S17-18), the sections were first rinsed with PB 0.1M and then permeated with PB 0.1 M and 0.3 % Triton X-100. They were then incubated overnight in a choleragenoid antibody raised in goat (goat anti-CTb, lot no. 703, List Biological Laboratories, USA) diluted 1: 2000 in a blocking solution (PB 0.1 M, 5 % BSA). …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Microinjection of CTB. Under general anaesthesia with isoflurane, a total of 3 µL 1% CTB (List Biological Labs, Campbell, California, USA) was used for injections at GB14, ST2 and ST6 in different rats (n=3 for each acupuncture point) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

EXPERIMENTAL PROCEDURES

Antigens and antibodies:

PTx (holotoxin) in glycerol was purchased from List Biological Laboratories, Inc. (Campbell, CA) …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Cholera toxin was purchased from List Biological Laboratories. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Cell stimulation:

RA synovial fibroblasts were seeded into 24 well plates and incubated at 37C, 5% CO₂ until confluent. The culture medium was replaced with 500l fresh complete RPMI 1640 medium (10% FCS, 400M L-glutamine, 2,000U/ml penicillin, 2mg/ml streptomycin, 1% v/v NEAA, 1% v/v sodium pyruvate, all from Sigma Aldrich, Gillingham, UK) prior to treatment with 10ng/ml of each of the cytokines IL-1, IL-17, IFN, TNF, TGF, IL-4 (all R&D Systems, Abingdon, UK), or IL-6 (Peprotech, New Jersey, USA) (20ng/ml), or TLR agonists 300ng/ml bacterial lipoprotein (bLP; InvivoGen, San Diego, US), 10g/ml poly(I:C) (InvivoGen, San Diego, US), or 100ng/ml LPS (List Biological Laboratories, Campbell, USA). The cells were incubated for 24hours at 37C, 5% CO₂ after which RNA was extracted for Gal9 expression as determined by Real time PCR.

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/