Bacterial Toxin Research Citations

… and ip injection with 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA) in 0.2 ml PBS on day 0 and 2. Animal weight and clinical score were monitored daily after symptom appearance. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

MOG-Induced EAE:

Mice were s.c. immunized with 50 µg of MOG35-55 peptide (Gene Script) emulsified in complete Freunds adjuvant (CFA) supplemented with 5 mg/mL Mycobacterium tuberculosis (Difco), followed by 150 ng of Pertussis toxin (List Biological Laboratories) administration on day 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis Toxin LIST Biological Laboratories Cat#180 …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Staphylococcus enterotoxin-B (SEB) assay:

Primary PBMCs (or mouse splenocytes) were obtained from healthy donor leukopacs (Stemcell technologies) and incubated +/ increasing concentrations of the super-antigen SEB (List Biological Laboratories), in the presence or absence of the PD1-Fc-OX40L ARC, human/mouse IgG (Jackson Immunoresearch, Inc.), antibody controls, or single-sided fusion protein controls (Acro Biosystems and Sino Biologicals); as both monotherapies and combinations. All antibodies and ARC proteins were used at the concentrations indicated in the figures. After 3days, culture supernatants were collected and assessed by ELISA for levels of IL-2 (R&D Systems). Additionally, the SEB assay was performed in human PBMCs depleted of CD4, CD8, or both CD4/CD8 cells, using magnetic positive selection kits (StemCell Technologies). …

… For the assays with stimulated rabbit PMNs, purified PMNs (1 x 106 cells/mL) were pre- 197 incubated for 1 h at 37C, 5% CO2 with lipopolysaccharide (LPS) purified from Escherichia coli O111 (500 198 ng/mL, List Laboratories). …

• Product #201 – LPS from Escherichia coli O111:B4

Recording solutions

… PTX (List Biological Laboratories, Inc., Campbell, CA) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin (List Biological Lab, USA) (300 ng) solved in 100 µl PBS and was administered intraperitoneally,   2 h after immunization. The pertussis toxin injection was repeated the next day. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and treatment of EAE model:

… Age matched mice were subcutaneously immunized with peptide MOG 35-55 (GL Biochem, Shanghai, China) mixed 1:1 with complete Freund’s adjuvant (Sigma, St. Louis, MO, USA), followed by pertussis toxin (200 ng; List Biological Laboratories, Campbell, CA, USA) administration i.v. on day 0 and day 2, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Llama immunization, serum fractionation and serology:

Recombinant TcdB (aa 17512366; hereafter referred to as TcdB_1751-2366) was a generous gift from Dr. Kenneth Ng (University of Calgary, Calgary, AB, Canada). A llama was immunized with 100 g of TcdB_1751-2366 per injection, following a similar immunization schedule and adjuvanted as previously reported [29]. Serum from blood drawn 42 days post immunization was tested for binding to TcdB_1751-2366 by ELISA essentially as described [10]. Serum was fractionated according to established protocols with protein G and protein A affinity columns [30] and conventional and heavy-chain IgG fractions tested for TcdB_1751-2366 binding by ELISA [10]. The ability of polyclonal fractions to neutralize TcdB VPI 10463 (List Biological Laboratories) was examined by Vero cell toxin inhibition assays, essentially as described for TcdA [19] with minor modifications. Vero cell monolayers were incubated with a final TcdB concentration of 10 pM (2.7 ng/mL) or 30 pM (8.1 pg/mL) and 1 M of day 42 fractionated serum for 72 h at 37C and 5% CO₂ before addition of WST-1 cytotoxicity reagent (Roche, Mississauga, ON, Canada) for 30 min and subsequent absorbance measurement at 450 nm.

• Product #155 – Toxin B from Clostridium difficile

… The sections were sequentially incubated for 3 days at 4C with goat anti-CTB antibody (1:10,000 dilution; List Biological Laboratories, Campbell, CA, USA, Cat# 703 subunit B from Vibrio cholerae type Inaba 569B, which was obtained from List Biological …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

List Labs has provided Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae) and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).  The former has been discontinued.  The latter may be substituted for the former, if the researcher takes into account the difference in the buffer in which each product is provided and the amount of protein per vial.  When Product #103B was reconstituted with 200 l of water, it contained 1 mg of protein in 0.05 M Tris, 0.2 M NaCl, 0.001 M Na₂EDTA, 0.003 M NaN_3, pH 7.5.  When Product #104 is reconstituted with 0.25 ml water, it contains 0.5 mg of Cholera Toxin B Subunit (choleragenoid) in 0.01 M Sodium Phosphate at pH 7.5.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt