Bacterial Toxin Research Citations

Toxin Activity Inhibition Assay:

… The cytotoxic titer was defined as the reciprocal of the highest dilution that produced rounding in 80% of Vero cells for each sample. Vero cells treated with purified C. difficile toxins (A and B) and antitoxin (List Biological Labs, 152C and 155C; TechLabs, T5000) were used as controls. A test cytotoxicity assay was run prior to assays to ensure that the 2-AI molecules did not affect the cytoskeleton of Vero cells at the tested concentrations.

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile

Pertussis Toxin Preparation:

Lyophilized pertussis toxin (List Biological) was reconstituted with distilled water. The reconstitute was then further diluted to a concentration of 2500 ng/ml then loaded into 1ml syringes for IP injection (Stromnes and Goverman, 2006).

EAE Induction:

Mice were shaved dorsally from the base of the tail to the middle of the back rostrally. Subcutaneous (SC) injections of MOG35-55/CFA emulsion were performed on day 0 and day 7. Each mouse received 4 subcutaneous (SC) injections of 0.05 ml at separate locations on the rump, yielding a total of 0.2 ml per mouse. Injections sites were arranged rostro-caudally, two on either side of the dorsal midline. A single IP injection 0.1ml of pertussis toxin was administered at a concentration of 2500 ng/ml on day 0 and day 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Pertussis toxin assay:

Pertussis toxin (List Biological Laboratories, Inc., Campbell, California) was activated by incubation …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Pertussis Toxin from B. pertussis, Lyophilized in Buffer List Biological laboratories, Inc Cat 180 …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

EAE Induction:

Active induction of EAE was performed by subcutaneous injection of nave 68 week B10.PL IL-3^/, IL-3^+/, and IL-3^+/+ littermate mice over four sites in the flanks with 200 µg MBP Ac1-11 (CS Bio, Menlo Park, CA, USA) emulsed in CFA (Difco, Becton Dickinson Co., Franklin Lakes, NJ, USA). Pertussis toxin (200ng) (List Biological Laboratories, Campbell, CA, USA) was injected i.p. at the time of immunization and 48h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Flow cytometry:

Cells were trypsinized, rinsed in cold PBS-BSA, and labeled with m825-MMAE, an isotype-matched human IgG, or FITC-conjugated protective antigen (List Biologicals Laboratories) in PBS-BSA at 4^o C. …

• Product #175 – Anthrax PA 63 – FITC Conjugate

… Escherichia coli lipopolysaccharide (O55:B5), a TLR4 agonist, was purchased from List Biologicals (USA). …

• Product #203A – LPS from Escherichia coli O55:B5 

– One hour prior to study drug administration, systemic inflammation was induced by bolus administration of 1 ng/kg Escherichia coli type O113 lipopolysaccharide (LPS; List Biological Laboratories Inc. Campbell, California, USA) …

• Product #9433 – GMP LPS Lipopolysaccharide List™ Hpt™ From Escherichia Coli Type O113

EAE Induction:

Mice (8-12 wk of age) were immunized subcutaneously with 100 µg MOG p35-55 (2 mg/mL in 1x PBS) in an emulsion (volume ratio 1:1) with CFA (2 mg/mL heat-killed Mycobacterium tuberculosis H37Ra; Difco Laboratories) as described previously (225). MOG p35-55 was synthesized by the Stanford Pan Facility (Stanford, CA). Mice were also injected intravenously with 125 ng Bordetella pertussis toxin (List Biological Laboratories) on day 0 and 2 post immunization. Mice were examined daily for clinical signs of EAE for 30 d and were scored on a five-point scale: 0, no clinical disease; 1, limp tail; 2, hindlimb weakness in one or both hindlimbs; 3, complete paralysis in one or both hindlimbs; 4, hindlimb paralysis plus some forelimb weakness; and 5, moribund or dead.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

Two doses of pertussis toxin (400 ng/mouse; List Biologicals, Campbell, CA) were given intraperitoneally: one on day 0 and the other on day 2 …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)