Bacterial Toxin Research Citations

Materials:

… Tetanus toxoid from Clostridium tetani List Biological Laboratories …

• Product #191 – Tetanus Toxoid from Clostridium tetani

Reagents and Antibodies:

… Anthrax lethal toxin was purchased from List Biological Laboratories, …

LDH cytotoxicity assays:

RAW 264.7 cells were seeded at 0.5 106 cells/well in 6-well plates in 15 
standard growth medium 24 hours prior to treatment. Cells were treated with the indicated agents
for the indicated times. Supernatants were then harvested and analyzed for LDH activity using an
LDH cytotoxicity assay kit (Pierce).  For experiments with proteasome inhibitors or bestatin
methyl ester, cells were treated with these agents 30 minutes prior to the addition of Val-boroPro
and/or LT, and then incubated for the indicated amount of time. …

Immunoblotting experiments:

RAW 264.7 macrophages were seeded at 0.5 106 cells/well in
6-well plates or at 5 106 cells/dish in 10 cm plates, and 24 h later were treated with agents as
described. Bestatin methyl ester and bortezomib, if used, were added 30 minutes prior to LT …

Immunoprecipitation experiments:

For immunoprecipitation experiments, HEK 293T cells were
seeded at 7 106 cells/well in 10 cm plates and transiently transfected with 5 g of N-terminal V5
and C-terminal FLAG-tagged Nlrp1b and 5 g of N-terminal HA-tagged ubiquitin using Fugene 10 
HD. After 24 h, cells were then treated with bortezomib for 30 minutes followed by LT or vehicle
for an additional 3 hours and harvested. …

Anthrax Lethal Toxin (LT) = Anthrax Lethal Factor (LF) + Anthrax Protective Antigen (PA)

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… SEB-stimulated (0.5 g/ ml; List Biological Laboratories) and unstimulated samples for each animal were included. …

Blood-brain barrier permeability assay:

Permeability across the blood-brain barrier was studied using a noninvasive whole body imaging InVivoXtreme 4MP (BRUKER, formerly Carestream). Brain permeability was assessed ex situ 24 hours after treatment in either control S. pneumoniaeinfected or pertussis toxin treated animals (200 ng/d from B. pertussis for 2 consecutive days treated as a positive control [#180 Islet activating protein, List Biological Laboratories]). …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Effect of αDCIR2-PLP139–151 fusion mAbs on the induction of EAE

To determine the therapeutic effect of DCIR2+ DC targeting on the clinical outcome of EAE, cohorts of 6 to 10-week-old female SJL/J mice were either left untreated or injected i.p. with 1μg of fusion mAbs (αDCIR2-PLP139–151 mAb, isotype control/ PLP139–151 mAb) 10 days before inducing EAE. Where indicated, cohorts of control mice were injected with 1 μg of conventional αDCIR2 mAb. For EAE induction, SJL/J females were immunized s.c. with 75 μg of synthetic PLP139–151 peptide emulsified in CFA. Pertussis toxin (200 ng, List Biological Laboratories, Campbell, CA) was administered i.v. on the next day. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… The CTb antibody (1:10,000, List Biological, cat#703) was a goat polyclonal raised against choleragenoid. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

LPS was purchased from List Biological Laboratories, …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

EAE:

… Mice also received 300 ng of Pertussis toxin (List Biological Laboratories) iv on the day of immunization and 2 days later. EAE score was …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin (List Biological Labs) in 100 ul saline was injected. intravenously twice, on days 0 and 2, and disease severity was assigned scores. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

EAE was induced in 8-week-old mice by subcutaneous immunization with 150g µ myelin oligodendrocyte glycoprotein (MOG3555) peptide (Genemed Synthesis Inc.) emulsified in complete Freunds adjuvant (CFA, Difco Laboratories) per mouse, followed by administration of 200ng pertussis toxin (PTX, List Biological Laboratories, Inc.) on days 0 and 2 as described …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)