Bacterial Toxin Research Citations

Reagents:

… Native Toxin B standard purified from C. difficile (ribotype 087) was purchased from List Laboratories (Campbell, CA). …

MultiPath C. difficile toxin B test:

To prepare the assay mixture, stool was diluted to 8% with a mix of stool diluent, 7e8 particles/mL of antibody conjugated magnetic particles, 1.1e7 particles/mL of antibody conjugated fluorescent particles, and the indicated amount of toxin B (diluted in 50mM Tris-HCl, pH 7.8 buffer containing 2mg/mL BSA, 0.05% w/v Tween-20 and 0.05% v/v Proclin-300) or just buffer for blank. …

Limit of detection, limit of blank, dynamic range, and precision profile:

These measurements were performed using the pooled negative stool sample. The limit of detection for the C. difficile MultiPath toxin B assay was determined by running 24 replicates of sample with no analyte and 12 replicates each of 7 toxin B concentrations. …

Testing C. difficile clinical samples:

320 clinical samples were tested as described above using a 3 well assay (test, positive control, and neutralization control for each sample). Each sample examined was tested independently by two operators. For the positive control, assay mixture inclusive of patient sample was spiked with 100pg/mLC. difficile toxin B to detect matrix inhibitory effects. …

• Product #155 – Toxin B from Clostridium difficile

MOG_35-55 induction of EAE and evaluation of clinical progression:

… The immunized mice were then injected intraperitoneally (i.p.) with two doses of 350 µg pertussis toxin (List Biological Laboratories, Inc., Campbell, CA, USA), on days 1 and 3 post-induction (dpi), with MOG₃₅₅₅. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Oral immunization

OVA, chicken egg, grade V (Sigma-Aldrich) was dissolved in sterilized PBS. Mice were orally administered 10 or 100 mg of OVA alone or plus 10 µg of CTA subunit, CTB subunit, or CT (List Biological Lab., Campbell, CA) or 10 µg of CTA subunit, CTB subunit, or CT alone in 0.3 ml of PBS.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Sections were incubated at 4 C for 48 h with 0.5% Triton X-100 in PBS containing goat polyclonal antiserum against CTB (1:20,000, List Biological Laboratories) and non-immune donkey serum (1:200) to prevent non-specific binding …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Four primary antibodies … or goat anti-cholera toxin subunit B (List Biological Labs, Inc. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Ultrapure LPS from Salmonella minnesota R595 (Re), List Biologicals, 434, …

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

Lipopolysaccharide administration (LPS):

Postnatal day 9 kits (n=6) received intraperitoneally-administered LPS (Escherichia coli O111:B4, List Biological, CA) at a dose of 1mg/kg every 12h for 3 doses. After the last dose of LPS, kits were exposed to hypoxia (6% oxygen for 45min) followed by hyperoxia (100% oxygen for 6h). Control animals (n=4) received equal volume saline injections and normoxia. Kits were sacrificed at P12.

• Product #201 – LPS from Escherichia coli O111:B4

Biological Material:

…Cholera Toxin (FAD)

8,3 ng/ml (working concentration)

List Biological laboratories, INC (Company)

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Materials:

… Botulinum Neurotoxin Type A (BoNT/A) List Biological Labs Cat# 130B …

• Product #130B – Botulinum Neurotoxin Type A from Clostridium botulinum

EAE induction and Treatment:

Female C57BL/6 mice of 812 weeks old were immunized subcutaneously with synthetic peptide derived from myelin oligodendrocyte glycoprotein (MOG₃₅₅₅) peptide (MEVGWYRSPFSRVVHLYRNGK, AnaSpec). Immunization was performed by mixing MOG₃₅₅₅ peptide in CFA containing 10mg/ml heat-killed H37Ra, strain of Mycobacterium tuberculosis (Difco Laboratories). Mice were immunized intraperitoneally with 250ng pertussis toxin (List Biological Laboratories) on the day of MOG injection and then again 48hours later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)