October 6, 2017
Journal Of Pharmacological Sciences
Product: Unspecified List Labs LPS
Experimental schedule:
Cells were stimulated with LPS (0.2 g/mL; List Biological Labo., Campbell, CA, USA) or nigericin … The expression of inflammasome-related molecules and the level of secreted IL-1 were analyzed by quantitative RT-PCR/western blotting and an enzyme-linked immunosorbent assay (ELISA), after culture for 6 h and 24 h, respectively. Trophoblasts were incubated with N6, 2 -0- dibutyryladenosine 3, 5-cyclic monophosphate (db-cAMP; 0.5 mM; SigmaAldrich) to enhance their fusion toward syncytiotrophoblasts in DMEM supplemented with 2% FBS for 2 days.
Author did not specify which List Labs LPS product was utilized in their research. List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/