Bacterial Toxin Research Citations

EAE Induction:

Mice were immunized subcutaneously (s.c.) in the flanks with an emulsion containing MOG3555 (100 µg/mouse) and M. tuberculosis H37Ra extract (3 mg/mL, Difco Laboratories) in CFA (100 L/mouse). Pertussis toxin (100 ng/mouse, List Biological Laboratories) was administered i.p. on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Sensitization of the CM:

In total, 35 healthy CMs (1 year old) were enrolled for the whole experiment (Table 1). Four groups (Group 1 to Group 4) were used for the intranasal administration of cedar pollen extract (100 µg/head) (Cosmo Bio, Tokyo, Japan) with cholera toxin (5 µg/head) (List Biological Labs. Inc., CA, USA) every 7 days. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Briefly, Nunc 96 well plates were coated overnight with 2 g/mL PT, 0.5 g/mL FHA, 0.2 g/mL FIM 2/3, or 2 g/mL PRN (List Biologicals, Campbell, CA). Horseradish peroxidase conjugated goat anti-monkey IgG polyclonal secondary antibody …

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

• Product #186 – Fimbriae 2/3 from B. pertussis
• Product #187 – Pertactin from B. pertussis (69 kDa Protein)

Reagents:

Purified lipopolysaccharides (LPS) from Escherichia coli was obtained from List Biological Laboratories Inc. (Campbell, CA, USA) and dissolved in endotoxin-free water …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

EAE:

… At 0 and 48 h after immunization, the mice were intraperitoneally injected with 400 ng/mouse of pertussis toxin (List Biological Laboratories, Inc., Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Abstract – …A fluorescent tag-labeled synthetic peptide (SNAPtide) derived from a segment of SNAP-25, an intracellular substrate of BoNT/A, is used to detect and assay the endopeptidase activity of BoNT/A.

List Biologicals developed a 13-mer peptide by replacing the Glutamine-194 of SNAP-25 with glutamic acid SNAPtide, which showed catalytic efficiency (Kcat/Km) of LCA as 28.5 10 4 M 1 sec 1 (Todd et al., 2005), which is comparable to full-length substrate GST 

List Labs provides the following SNAPtide products:

• Product #520 – SNAPtide Peptide Substrate (o-Abz/Dnp) for C. botulinum Type A Neurotoxin
• Product #521 – SNAPtide Peptide Substrate (FITC/DABCYL) for C. botulinum Type A Neurotoxin

Heptanol abrasions & tracer applications:

… One week after abrasion, 1% Cholera toxin B (CTb; List Laboratories, Campbell, CA) was applied to the cornea using a similar method, but with a smaller metal ring (7-gauge (3.8 mm ID), HTX-07R, Small Parts, Inc.).  For both tract tracer applications, the dye was left on the cornea for 30 peer-reviewed) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was not. http://dx.doi.org/10.1101/242685doi: bioRxiv preprint first posted online Jan. 4, 2018;
5 minutes [22].  Animals were perfused 1 week after CTb application as described below and trigeminal ganglia
(TG) were examined for the presence of single (CTb)- or dual (CTb and FG)-labeled neurons (see section 2.5).

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Treatment with inflammatory inducers:

The cells were incubated 3 to 7 days in monolayer culture and were then stimulated with recombinant IL-1 (5 ng/mL, R&D Systems) or LPS (10 ng/mL, Escherichia coli 055:B5, List Biological Laboratories, CA, USA) for 24 h. Unstimulated cells were used as controls.

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

… Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native, List Biological Laboratories, Cat#169A. Anthrax Protective Antigen (PA), Recombinant from B. anthracis, List Biological Laboratories, Cat#171E. …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

List Labs Product #169A (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence; Lyophilized) has been discontinued; however, Product #169L (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence; Liquid) is available for purchase.

• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

… Pertussis Toxin (PTX) (List Biological Labs, Campbell, CA, USA), …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)