Bacterial Toxin Research Citations

EAE induction using myelin oligodendrocyte glycoprotein35–55 peptide

Reagents for EAE induction were prepared by dilution of myelin oligodendrocyte glycoprotein35–55 peptide (MOG; 2 mg/mL; AnaSpec, Fremont, CA) in UltraPure™ distilled water (Invitrogen, Life Technologies, Grand Island, NY) emulsified with equal parts of complete Freund’s adjuvant (BD Biosciences Franklin Lakes, NJ) containing 8 mg/mL Mycobacterium tuberculosis H37RA (#231131; BD Biosciences). MOG emulsion was transferred to 1 mL Luer-Lok syringes (BD Falcon) with 27G ½” needles. Pertussis toxin was diluted in UltraPure™ (2 ng/μL; List Biologicals Laboratories, Campbell, CA) and transferred to syringes as described above. While anesthetized under 5% isoflurane gas, 6- to 8-week-old female C57Bl/6 mice (Charles River Laboratories, Wilmington, MA) were induced with EAE by subcutaneous injection of 100 μL MOG emulsion near flanking regions of the tail (200 μL total per mouse). Mice were concomitantly administered 100 μL pertussis toxin via intraperitoneal (IP) injection. After 48 h, mice received a second IP injection of 100 μL pertussis toxin to complete the induction procedure. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Purified proteins … Prn, PT for ELISA assays were from List Biologicals. …

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

• Product #187 – Pertactin from B. pertussis (69 kDa Protein)

EAE:

… Pertussis toxin (200 ng) (List Biological Laboratories, Campbell, CA) in PBS and 50 mM NaCl were administered ip on the day of immunization and again 48 h later. After induction, neurological deficits …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… The intraperitoneal treatment with diphtheria toxin (DT; List Biological Laboratories, Inc …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… A goat anti-cholera toxin, subunit B (CTB) antibody (List Biological Laboratories, Campbell, CA; #703, Lot 7032AA, …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE:

… On days 0 and 2 post-immunization, 200 ng of pertussis toxin (PT) (List Biological, Campbell, CA) was administered intraperitoneally (ip) in 0.2 ml of PBS (Sigma, St. Louis,Minnesota). Mice were observed daily …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… CRM (List Biological Laboratories Inc., Campbell, CA) …

• Product #149A – Diphtheria Toxin CRM197, Mutant

EAE induction.

Adult mice were injected s.c. in each hind leg with 50 μg of MOG35–55 (Peptide Synthesis Facility, University of Alberta) emulsified in 100 μl CFA (MilliporeSigma). Animals received 0.3 μg/ml of pertussis toxin injected i.p. Injection of pertussis toxin (List Biological Laboratories) occurred on the day of induction with MOG35–55, with subsequent infection 48 hours later. Control animals were immunized with CFA and PBS (1:1) and pertussis toxin, as above. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE and treatments:

… EAE was induced using a modification of our previously reported method [8, 9]. In brief, 8 to 10 weeks old C57BL/6 female mice were subcutaneously injected with 100 µg myelin oligodendrocyte glycoprotein (MOG) ₃₅₅₅ emulsified in complete Freund’s adjuvant (CFA) supplemented with intraperitoneal injections of 400 ng pertussis toxin (List Laboratories, Campbell, CA, USA) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE and clinical evaluation:

For the induction of EAE, wild-type (WT) and Slfn8^/ mice (C57BL/6 and 129SV mixed background) were immunized subcutaneously with 150 µg of myelin oligodendrocyte glycoprotein (MOG) peptide (MOG_35-55) (Anaspec, Fremont, CA, USA) emulsified in CFA (day 0). After immunization, the mice were intraperitoneally (i.p.) injected with 200 ng of pertussis toxin (List Biological Laboratories, Campbell, CA, USA) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)