Bacterial Toxin Research Citations

4.1. Tetanus Toxin Labeling

Alexa Fluor®647 was attached to the tetanus toxin ((Cat. no. 19050A2; List Biological—Campbell, CA, USA), referred to TeNT-647, according to the manufacturer’s protocol from Alexa Fluor®647 Labeling Kit (Cat. no. A30009; Thermo Fisher Scientific—Waltham, MA, USA) and stored at −20 °C. Quantifying the Alexa Fluor®647 ratio per tetanus toxin was obtained by scanning measurements using the NanoDrop One c device (Thermo Fisher Scientific—Waltham, MA, USA) with 280 and 650 nm readings. The average dye per mole of protein obtained was 1.7 moles.

• Product #190 – Tetanus Toxin from Clostridium tetani

… Diphtheria Toxin List Labs 150 …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Neural tract tracing

For targeting the nTS, the brainstem was exposed via a limited occipital craniotomy. Glass micropipettes containing either retrograde
tracer cholera toxin subunit B conjugated to AlexaFluor 555 (CTB; 1% in deionized water, List Biological Laboratories) or latex beads (RetroBeads, Lumafluor) were nanoinjected into the PVN (n = 8) or nTS (n = 12) bilaterally (200 nl for PVN; 60 nl for nTS) using the follow…

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Pertussis Toxin List Biological Laboratories Inc. Cat#180 …

Experimental Models and Tamoxifen delivery

… All adult mice for 5xFAD and cuprizone labeling experiments received injections every other day for 3 days, followed by 3 daily injections and sacrifice. For EAE experiment, Clec7a-CreERT2;LSL-tdTomato mice were aged to 2-4 months and were immunized with 200ug of MOG (35-55), 1mg/mL of complete Freund’s adjuvant supplemented with BD Difco Mycobacterium Tuberculosis H37 Ra Dessicated and 300ng pertussis toxin. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… Mice were also injected intraperitoneally with 500 ng of pertussis toxin (PTX) (List Biological Laboratories Inc.) on the day of immunization (day 0) and the following day …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… 96-well plates (Millipore) were pre-coated with PT (5 ug/ml; list biological) overnight. Then 1*10^5 cells per well were cultured and assayed using the ELISpot reader …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Complete Freund?s Adjuvant (CFA) and heat-killed Mycobacterium tuberculosis H37Ra were purchased from Sigma. Pertussis toxin was purchased from List Biological Laboratories. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell lines

The following commercially available cell lines were used: … 100 ng/mL cholera toxin (List Biological Laboratories, 100B)). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE induction.

Littermate female WT, heterozygous (Fbln2+/–), and homozygous (Fbln2–/–) mice (10–12 weeks old) were injected subcutaneously with 50 μg MOG35–55 peptide (synthesized by Protein and Nucleic acid Facility, Stanford University, Stanford, California, USA) emulsified in CFA (Thermo Fisher Scientific) supplemented with 10 mg/mL heat-inactivated Mycobacterium tuberculosis H37Ra (MilliporeSigma). A total of 100 μL emulsion was injected at 2 sites into hind flanks. Pertussis toxin (300 ng per 200 μL; List Biological Laboratories, 180) was intraperitoneally injected on day 0 and 48 hours after MOG immunization. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… Toxoid tetanus was purchased from List Biological Lab (USA) …

• Product #191 – Tetanus Toxoid from Clostridium tetani