Bacterial Toxin Research Citations

EAE induction. Mice were s.c. injected into both flanks with a total of 200 μl of emulsion containing either 300 μg of MOG35–55 (Synpeptide) or 500 μg of bMOG (see above) dissolved in saline and mixed with an equal volume of complete Freund’s adjuvant containing 500 μg of killed Mycobacterium tuberculosis H37 RA (Difco Laboratories). Mice were also i.p. injected with 20 μg/kg of PTX (List Biological Laboratories) immediately and 2 days after immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Diphtheria Toxin List Biological Labs 150

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Mouse primary corneal epithelial cells (MPCEC)

… Each cornea was cut in half and placed in a 35 mm dish (Fisher Scientific, PA) with the epithelial side up. After approximately 5 minutes in the laminar air-flow culture hood, the half corneal button attached itself to the bottom of the plate, and 1.5 mL of DMEM (Life technology, NY) with 10% serum containing 40 μg/mL gentamicin (Life technologies, NY), 1% ITS (BD Biosciences, Bedford, MA), and 100 ng/mL cholera toxin (LIST Biological Laboratories, Inc., Campbell, CA) were added and the tissue was placed in a humidified incubator at 37 °C with 5% CO2. Culture medium was replaced every 2 days. After 7 to 10 days cells were nearly 100% confluent. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE:

Mycobacterium tuberculosis (H37Ra; Difco Laboratories). In addition, all mice were given 200 ng pertussis toxin (List Biologicals Laboratories) intraperitoneally on days 0 and 2 post-immunization (pi). Mice were graded for clinical 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Antigens:

… Recombinant protective antigen (rPA) was obtained from List Biological Laboratories (Campbell, CA). Each vial was reconstituted  

Quantification of anti-PA IgG antibodies:

Wells of an Immulon 2 HB, 96-well round bottom microtiter plate (Southern Biological, Birmingham, AL) were coated with 100 l of a coating solution (rPA diluted at 1 g/ml in a 0.01 M PBS solution, pH 7.4). …

Titration Curve:

Eight rPA-coated microtiter plates were prepared. Following overnight incubation, approximately 200 l of REF plasma that had been diluted to 1:500 was added to the first well of three rows on each plate then two-fold serial dilutions carried out. …

Specificity:

In the second experiment, two rPA coated microtiter plates were prepared, except that ovalbumin (100 l diluted at 1 g/ml in a 0.01 M PBS solution), a heterologous protein to B. anthracis PA, was also used to coat six wells in each plate. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… LPS (List Laboratories, Los Gatos, CA), …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/

… Mice also received intraperitoneal (ip) injections of 200 ng Bordetella pertussis toxin (List Biological, Campbell, CA) on days 0 and 2. For induction of passive EAE by adoptive transfer, donor mice were immunized sc with 200 µg of MOG35-55 in CFA …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

IL-27HA and IL-35HA gene therapy:

Chronic EAE was induced in female C57BL/6 mice by subcutaneous immunization with 300µl of 200µg per mouse of MOG3555 in Freunds Adjuvant Incomplete liquid, IFA, (Sigma) supplemented with 8mgml1 Mycobacterium tuberculosis (strain H37Ra; Difco, Lawrence, KS, USA). Pertussis toxin (500 ng, List Biological Laboratories, Campbell, CA, USA) was injected i.v. on the day of the immunization and again 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mouse model of neonatal WMI:

Neonatal unilateral WMI was induced in C57BL/6 mice (Envigo) by inducing a hypoxic-ischemic insult, directly followed by an inflammatory stimulus, as adapted from Shen et al.^20,21. Briefly, at P5 HI+LPS mouse pups (n=6) underwent permanent occlusion of the right common carotid artery under isoflurane anesthesia, followed by exposure to 35minutes of hypoxia (6% oxygen). Immediately after hypoxia, pups received 1mg/kg LPS i.p. (List Biological Laboratories, Campbell, CA). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Vaccination regimen with ovalbumin as antigen

Ovalbumine (OVA) is a commonly used antigen in order to assess adjuvant activity [19,32]. In two distinct assays, a total of fourteen mice per group received intra-rectally (IR) 20μg of ovalbumin (ovalbumin grade VII, Sigma) with 7μg of adjuvant, either cholera toxin (CT) (List Biological Laboratories, INC), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae