Bacterial Toxin Research Citations

Surgery:

… For VP cell lesions or retrograde NAc labelling, mice were unilaterally injected into the VP (0.4 mm anterior and 1.3 mm lateral to bregma, 4.3 mm below the dura) with 5% ibotenic acid (30 nl per injection; Tocris, 0285) or 0.2% CTb (18 nl per injection; List Biological, Cat# 104), respectively. …

Histology:

Immunohistochemistry was performed on free-floating sections as described previously¹⁴. In brief, sections were rinsed in PBS, incubated in 0.3% hydrogen peroxide in PBS for 30min at room temperature, and then sequentially at room temperature in 3% normal donkey serum and 0.25% Triton X-100 in PBS (PBT) for 1h and primary antibody diluted in PBT with 0.02% sodium azide overnight. After overnight incubation with … or goat anti-CTb antibody (1:15,000; List Biological, Cat# 703), sections were rinsed and incubated for 2h in biotinylated antibody …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… For the experiments involving the Gi inhibitor, PTX (List Biological Laboratories Inc., Campbell, CA) and 8-Br-cAMP (Sigma-Aldrich), cells were pre-treated with the two compounds for 30 min …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… For EAE induction incomplete Freund’s adjuvant (IFA) and killed Mycobacterium tuberculosis strain H37RA were purchased from Difco (Sparks, MD), and pertussis toxin was purchased from List Biological Laboratories (Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Cholera Toxin (100 ng/mL) (List Biological Laboratories, Campbell, CA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE:

… 200 ng of pertussis toxin (List Biological Lab, Epsom, England) was given intraperitoneally on days 0 and 2 post-immunization (pi). EAE was assessed by a 0-5 scoring system described previously (Stromnes and Goverman, 2006). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

– … solution; #103, List Biological Laboratories, INC, Campbell, CA) or Fluorogold (2.5-5.0% FG, … They were then incubated in the goat anti-CTB antiserum (List Biological Cat# 703 RRID: AB_2313637) at 1:2,000 for 2 days at 4°C. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE induction and treatment:

EAE model was induced as previously described [41]. Briefly, Female mice at 8-10 wk of age were immunized subcutaneously in the flanks with 200 µg of MOG_35-55 peptides (purity >98%, GL Biochem Ltd., Shanghai) which were emulsified in complete Freunds adjuvant (CFA) containing 500 µg of Mycobacterium tuberculosis H37RA (Difco Laboratories, USA). Furthermore, 400 ng of pertussis toxin (PTX, List Biological Laboratories, USA) in 100 µl PBS-50 mM NaCl was intraperitoneally injected on the day of immunization and 24 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Electrophysiology:

… For the inflammatory model in adult DRG cultures, trinitrophenyl conjugated-lipopolysaccharide (TNP-LPS) conjugate from Escherichia coli (List Biological Laboratories, Campbell, CA, USA) was resuspended in media 1mg/mL and added to cultures (20g/mL). …

Author has indicated the following LPS used:

• Product #201 – LPS from Escherichia coli O111:B4

This paper provides valuable information regarding effects of ACT on phagocytes.

• Product #188L – Adenylate Cyclase Toxin, Recombinant from B. pertussis

… LPS from E. coli O111:B4 was purchased from List Biological Laboratories, Inc. …

• Product #201 – LPS from Escherichia coli O111:B4