Bacterial Toxin Research Citations

Calcium imaging:

…After imaging for approx. 5 min. to establish baseline conditions, ligands were superfused onto the cells as indicated in figures using a gravity-driven perfusion system. For some experiments, cells were pretreated with pharmacological substances (H89, KT5720, BIM, mSIRK or mSIRK-L9A, Figure 6 and Figure 7 figure supplement 3), for 30 min. by adding them to the culture medium during Fura2AM loading. Pertussis toxin (PTX, 100 ng / ml, List Biological Laboratories, Campbell, CA, USA) was added to the culture medium in the incubator for 16 24 h and washed off the extracellular medium before loading with Fura2-AM. … 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…Mice received 150 ng pertussis toxin (List Biological Laboratories) intraperitoneally on days 0 and 2. Passive-transfer EAE was induced by injection of 2D2 TH17-polarized cells (as described above) intravenously into recipient mice at 5 106 to 7.5 106 cells/mouse. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

For the induction of peanut extract (PE) -induced food allergy, C57BL/6 mice were sensitized orally once a week for four weeks with 2 mg PE adjuvanted with 20 µg cholera toxin (List Biologicals 101B) followed by either four oral challenges on four consecutive days with 10 mg PE (in this model, symptoms were scored after the first three challenges) or two i.p. challenges (with a two -day break) with 1 mg PE (in that case, symptoms were scored after the first challenge). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Neutralizing Antibody Assay:

Toxin neutralizing properties of antiserum were determined using Vero cells and C. difficile purified toxin B (List Biological Labs, Campbell, CA, United States).

• Product #155 – Toxin B from Clostridium difficile

…Endotoxin-free, ultrapure lipopolysaccharide (LPS) was purchased from List Biologicals (Campbell, CA). …

Author specified the following ultrapure LPS used in their research:

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

… Human tumor necrosis factor-alpha (TNF-), interleukin (IL)-1, IL-6 (Peprotech, Rocky Hill, NJ) and lipopolysaccharides (LPS; List Biological Labs, Campbell, CA) were used for the pro-inflammatory cytokine stimulation, in FBS-free MEM. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Adjuvants:

…The cholera toxin (CT) adjuvant used was the B subunit of CT, pre-clinical grade (List Biological Laboratories, USA), supplied as lyophilised powder and reconstituted according to the manufacturers instructions. The adjuvant was mixed with the viral vectored vaccine by gentle pipetting and administered at doses and routes indicated in the Results and the Figures.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Induction and monitoring of EAE:

…EAE was induced in 8- to 12-week-old female hCCR6-Tg/mCCR6^/ mice by immunization with 75 g of recombinant rmMOG emulsified in Complete Freund Adjuvant (CFA, Sigma-Aldrich) containing 4 mg/ml Mycobacterium tuberculosis (Difco) subcutaneously on the inner side of both hind flanks. Mice were given 350 ng of pertussis toxin (List Biological Laboratories) i.p. on day 0 and again 48 hours later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction:

Active EAE was induced in C57BL/6 female mice by subcutaneous (s.c.) injection of 200 g myelin oligodendrocyte glycoprotein (MOG)3555 peptide (United Biochemical Research, Seattle, WA, USA) in 50 L of complete Freunds adjuvant (CFA) containing 5 mg/mL Mycobacterium tuberculosis H37Ra (Difco Laboratories, Detroit, MI, USA). Mice also received intraperitoneal injections of 400 ng Bordetella pertussis toxin (PTx) (List Biological, Campbell, CA, USA) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mouse breeding and treatments:

…DT (List Biological Laboratories Inc.) was injected i.p. at 10 ng/g of body weight every 48 hours for a total of 4 injections. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae