Bacterial Toxin Research Citations

Induction and evaluation of experimental autoimmune encephalomyelitis

Mice were 8 to 14 weeks of age at the time of immunization. Experimental autoimmune encephalomyelitis (EAE) was induced by subcutaneous immunization with 60 to 75 μg recombinant human myelin oligodendrocyte glycoprotein protein (rhMOG, AnaSpec) and 800 μg H37Ra (DIFCO Laboratories) emulsified in complete Freund’s adjuvant (DIFCO Laboratories) or 200 μl of recombinant human MOG1–125 Hooke-Kit (Hooke Laboratories) followed by two subsequent intraperitoneal injections of 300 ng pertussis toxin (List Biological Laboratories or Hooke Laboratories) at the time of immunization and respectively one or two days later. In some experiments 400 ng pertussis toxin was used, while taking care that controls and testing cohorts received the same amount. Boost was performed four to six weeks after immunization via a second subcutaneous injection with half the amount of the components from the primary EAE induction. Some mice were boosted with complete Freund’s adjuvant and Mycobacterium tuberculosis only. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… transganglionic tracer, cholera toxin -subunit (CTB; List Biologicals; 1%; 2 µl per side) bilaterally into the sciatic nerves, 3 days prior to perfusions  (List Biological, 1:80.000 in TBST, 1% horse serum) for 72 h at 4° C, washed 3x in TBS with 1% horse …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

in the culture wells with the highest drug concentration did not exceed 1%. Cocultures were exposed to neostigmine (Sigma) and pancuronium (Sigma) for 10 minutes, and to acetylethylcholine mustard hydrochloride (Sigma), BoT-A (List Biological Laboratories) and 

EAE Induction:

… The day of MOG immunization was designated as day 0. Mice received intravenous injections of 400 ng of B. pertussis toxin (List Biological Laboratories) 0 and 2 days post-immunization. Control animals received B. pertussis toxin and emulsion containing PBS/CFA without MOG. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Animals received two intraperitoneal (IP) injections of pertussis toxin (List Biological Laboratories; #179B) …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunization Procedures:

Peptide emulsions were prepared in complete Freunds adjuvant (CFA) containing Mycobacterium tuberculosis H37RA extract (Difco Laboratories, Detroit, MI, USA) to a final concentration of 5 mg/ml. To induce disease, animals were immunized twice s.c., in inguinal and sternal regions on days 0 and 7, and all animals received pertussis toxin (PT, List Biological Laboratories, Campbell, CA, USA; 100 ng/mouse) i.p., on days 0 and 2 after the first immunization …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

For T reg cell depletion by DT, Foxp3EGFPDTR+ mice were treated i.p. with 50 g/kg DT (List Biological Laboratory).

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Cell culture and transfection:

The normal epithelial breast cell line MCF-10A was purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA) and cultured in MEBM basal medium (Lonza, Basel, Switzerland) supplemented with the MEGM Single Quot Kit (Lonza) and cholera toxin (List Biological Labs, Campbell, CA) under a humid environment with 5% CO₂ at 37°C. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… SC injections were centered on segments C2-3 and C6-7 in different rats. Tracers used were cholera toxin B subunit (CTB; 1% in saline, List Biological, Campbell, CA, product #103B, lot #10327A) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Induction and evaluation of EAE:

Male C57BL/6 mice (6-8 weeks old), originally obtained from the Jackson Laboratory, were injected s.c. in both flanks with 100 µg of the MOG₃₅₅₅ peptide (MEVGWYRSPFSRVVHLYRNGK) that was dissolved in PBS, emulsified in an equal volume of CFA (Difco), and supplemented with 5 mg/ml heat-killed mycobacterium tuberculosis H37Ra (Difco). The mice were also injected twice i.p. with 200 ng of pertussis toxin (List Biological Laboratories), once on the day of immunization and once 24 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)