Bacterial Toxin Research Citations

Toxins:

TcdA and TcdB₍₀₀₃₎ were purchased from List Biological Laboratories …

Murine passive protection:

Female C57BL/6 mice (78 weeks old) were administered 2 mg anti-T-toxin hIgG or PBS IP. Mice were challenged IP 24 h later with a MLD_100% of TcdA, TcdB₍₀₀₃₎, or CDTa plus CDTb and observed for 7 days.

• Product #152C – Toxin A from Clostridium difficile 
• Product #155 – Toxin B from Clostridium difficile

Macrophage culture and activation:

Murine peritoneal macrophages were elicited with thioglycollate (BD, Sparks, MD, USA; same batch for all strains) for 4 days. Cells from up to 4 mice were pooled and plated at 4 10⁶ cellsml¹ in DMEM+20% FBS+1% streptomycin/penicillin in duplicates or triplicates. After overnight culture, adherent cells were selected (adherence assay for macrophage enrichment). RNA deconvolution shows two main populations of inflammatory macrophages (F4/80+, MHCII and F4/80, MHCII+) and a small population of neutrophils (Supplementary Fig. 2). Cells were incubated with 2 ngml¹ LPS (List Biological, Campbell, CA, USA) or control in DMEM+1% FBS for 4h before harvest. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Induction and clinical assessment of EAE:

…Mice were immunized subcutaneously in both sides of the flank with 200 g MP4 in 200 l CFA. Mice received intraperitoneal injections of 200 ng pertussis toxin (List Biological Laboratories, Hornby, ONT, Canada) on the day of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Mice were immunized with a MOG 35-55 kit (EK-2110; Hooke Laboratories). Each mouse was immunized with a 20-L sc injection of MOG 35-55 /CFA emulsion and an ip injection of 200 ng of pertussis toxin (List Biological Laboratories) diluted in sterile PBS (14). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE disease model:

The experimental autoimmune encephalomyelitis (EAE) model was used as previously described⁴⁵. Briefly, 7-8 week old female mice were immunized s.c. with 200g MOG₃₅₅₅ (Life Technologies) in complete Freunds adjuvant (Difco). Mice were given 400ng pertussis toxin (List Biological Labs) in 500l PBS i.p. on day 0 and 2 post-immunization. For adoptive transfer, 110⁶ WT Foxp3 (GFP⁺) iTreg cells or PD-1HKO Foxp3 (GFP⁺) iTreg cells were injected i.v. before immunization on day 0. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…Mice were injected iv with 300 ng of reconstituted lyophilized Pertussis toxin (List Biological Laboratories) on the day of immunization and two days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…The MDS formulations were prepared with the following parameters: Matrix materials: poly (dl-lactide-co- glycolide) and poly (dl-lactide) Microsphere mean diameter range: 6-20 m RPA of anthrax (from List Biological Laboratories, Campbell, CA, US …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Pertussis-Toxin List Biological Laboratories, Campbell, CA, USA …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

The following inhibitors were used: PTX (List Biological Laboratories, 100 ng/mL), …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Tracing of myelinated afferent fiber projections to the dorsal horn:

The transganglionic tracer, cholera toxin B-subunit (CTB), has been used to map the central projections of cutaneous myelin primary aerents in the spinal dorsal horn of rats.25 After six weeks of RTX injection, CTB (1%, List Biological Laboratories, Campbell, CA) was injected into left sciatic nerve of vehicle-, RTX-, RTXMDL28170-, and RTXPEG400/DMSO-treated rats to determine the eect of MDL28170 on RTXinduced sprouting of myelinated aerent bers labeled by CTB in the dorsal horn. The sciatic nerve was exposed at the level after the rats were anesthetized with 10% chloralic hydras (3.5ml/kg, intraperitoneal). Four microliters of CTB tracers were loaded into Hamilton Yuan et al. 3 microsyringes and injected into the sciatic nerves on the left side of the body. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt