Citations

Cholera toxin production assay:

GM₁ ganglioside enzyme-linked immunosorbent CT assays (ELISAs) were performed on the supernatants of cultures grown under AKI-inducing conditions or soy LB rhamnose-inducing conditions, and the total ng of CT produced per ml of culture per OD₆₀₀ unit (ng CT ml¹ OD₆₀₀¹) was determined as previously described [31]. Purified cholera toxin B subunit (List Biological Laboratories, Campbell, CA) was used as a standard.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… difficile toxin B (product number #155A), Staphylococcus aureus alpha toxin (product number #120C), Staphylococcus aureus enterotoxin type B (product number #122C), Salmonella typhimurium lipopolysaccharide (product number #225, all List Biological Laboratories …

• Product #155- Toxin B from Clostridium difficile
• Product #120 – Alpha Toxin from Staphylococcus aureus
• Product #122 – Enterotoxin Type B from Staphylococcus aureus
• Product #225 – LPS from Salmonella typhimurium

To induce EAM, mice received subcutaneous immunizations on days 0 and 7 of 100 g myosin heavy chain (MyHC)614629 peptide… On day 0, mice also received 500 ng pertussis toxin intraperitoneally (List Biologicals; …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

 Analysis of serum and mucosal antibodies:
 
Serum antibodies were analyzed by ELISA. High-affinity ninety-six well plates
designed for EIA/RIA (Thermo Scientific, Waltham, MA) were coated with 0.5 g/ml of
purified C. difficile Toxin A Toxoid and Toxin B Toxoid (List Biologicals, Campbell, CA) for 24
hours. Non-specific binding was blocked by incubating coated plates with 200 l of 5%BSA in
PBS-T for at least 2 hours at room temperature. Serum, mucosal extract or supernatant from …

…Cholera toxin (List Biological Laboratory, No. 100B) ! CAUTION Cholera toxin is potentially harmful. Wear gloves and handle it carefully in a biosafety cabinet. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

…Briefly, the sections were incubated at room temperature with a mixture of goat anti-CTb Ig (1:2,000; List Biological, Campbell, CA, USA), …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Cholera Toxin B Subunit (CtB), Goat, Polyclonal, Purified CtB. isolated from Vibrio. cholerae. List Biological. Laboratories, Campbell, CA, USA. Catalogue number 703. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

– … The cells were stimulated with 10 g/L ultra-pure, endotoxin-free LPS (List Biological Laboratories, Inc, Campbell, CA, USA) per well. An equal volume of sterile endotoxin-free water was added to the negative control wells. …

Author indicated that the following List Labs product was utilized:

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

EAE:

EAE was induced in male and female B6 and PWD mice as follows. Mice were injected subcutaneously with 0.1 ml of emulsion containing 2.5 mg of MSCH in PBS and 50% CFA (Sigma, USA) on day 0 and day 7. CFA was supplemented with 4 mg/ml Mycobacterium tuberculosis H37Ra (Difco, USA). On day 0 and day 2 mice also received an i.p. injection of 200 ng pertussis toxin (List Laboratories, USA) as an ancillary adjuvant. Starting on day 10, mice were scored visually as previously described …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Mice received 100 ng pertussis toxin (List Biological Laboratories, Campbell CA) ip on days 0 and 2. Disease severity was evaluated using the following scale: 1: flaccid tail; 2: impaired righting reflex and hindlimb weakness; 3: partial hindlimb paralysis; 4: complete …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)