Bacterial Toxin Research Citations

EAE:

…On days 0 and 1 post-challenge, mice received 400 ng of Bordetella pertussis toxin intraperitoneally (i.p.) (List Biological Laboratories, Campbell, CA; provided with Hooke Kit TM for EAE Induction) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Serology:

…Toxin-neutralizing assay (TNA) was performed as published earlier [53] to qualitatively assess the ability of antibodies generated to neutralize lethal toxin. Briefly, heat inactivated individual sera were serially diluted in 8 log₂ steps with a starting dilution of 1:50 on sterile 96 well plates (BD Biosciences, Heidelberg, Germany) seeded with 10⁵ J774 A.1 macrophages per well. Lethal toxin (LF) was added at concentration of 500 ng/mL PA and 100 ng/mL LF (List Biological Laboratories, Campbell, CA, USA) and survival of macrophages was visualized through MTT (5 mg/mL, Alfa Aesar, Karsruhe, Germany), which is degraded by living cells to a measurable (OD540_nm) colour compound. …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Determination of mAb affinity by surface plasmon resonance:

The affinities of mAbs were determined by surface plasmon resonance (SPR) on a BIAcore T200 instrument (GE Healthcare, Uppsala, Sweden) by carrying out two independent multi-cycle kinetic measurements for each antibody. All analyses were performed at 25°C on a CM5 sensor chip in running buffer (10 mM HEPES, 150 mM NaCl, 3 mM EDTA, 0.05% Tween 20). Polyclonal anti-mouse IgG antibody was covalently conjugated to a CM5 sensor chip according to the manufacturers protocol (Mouse Antibody Capture Kit, GE Healthcare, Uppsala, Sweden). Individual mAbs (1 µg/mL) were bound to this mouse capture chip for 30 s at 5 µL/min. Each captured antibody was subjected to five consecutive threefold serial dilutions of epsilon toxin (ranging from 2.47 to 1800 nM; List Biological Laboratories) for 120 s at a constant flow rate of 30 µL/min to obtain a maximum signal of approx. 30 resonance units (RU). …

Experimental design:

Host-cell free A. phagocytophilum obtained from 3  10⁷ Hoxb8 neutrophils with an infection rate of 90% was used to infect 1.2  10⁷ Hoxb8 neutrophils (differentiated for 4 days) in 6 ml medium. … Depending on the experiment, cells were stimulated with 10 ng/ml or 200 ng/ml Escherichia coli K12 D31m4 (Re) LPS (List Biologicals, Campbell, CA, USA) or …

• Product #302 – LPS from Escherichia coli K12, D31m4 (Re)

EAE:

…containing heat-killed Mycobacterium tuber- culosis (Mtb, Difco, Detroit, MI) subcutaneously at four sites on the flanks followed by intraperitoneal injections of pertussis toxin (Ptx, List Biologicals, Campbell, CA), 75 ng on the day of immunization …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…CTB … 2% List Biological 103B …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

EAE:

…peptide in CFA containing 10 mg/mL heat-killed H37Ra, strain of Mycobacterium tuberculosis (Difco Laboratories). Animals received 250 ng pertussis toxin (List Biological Laboratories) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Neonatal CTB Injections for Anterograde Axonal Tracing:

Ferret kits aged P2 (n = 3) and P4 (n = 2) were deeply anesthetized and immobilized on a Kopf small animal stereotaxic apparatus. A borosilicate micropipette (20 m outer ) containing 2% CTB in PB pH 6.0 (List Biological Labs, Campbell, CA) was lowered into the LGN (1.2 mm posterior to Bregma; 1.11.2 mm lateral to midline; 4.85.5 mm under the pial surface). CTB was injected by iontophoresis applying positive current pulses 2 Amp, 7 on/off for 10 min. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

…Cholera toxin (CT) was purchased from List Biological Laboratories. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

LPS activation (control) is shown in the two bars to the right of the panel with an additional mock-coincubated control (100 ng ultrapure E. coli LPS [List Laboratories] was added to the cells for 3 h).

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/