Citations

EAE:

… Pertussis toxin (500 ng/mouse) (List Biological Laboratories, Inc.) was injected intraperitoneally on days 0 and 2. Animals were monitored daily for EAE signs based on a standard EAE 0-5 scale scoring system: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Diphtheria toxin administration:

Diphtheria toxin (DT) (List Biologicals Laboratories, Campbell, CA) was suspended in PBS. Stock solutions were thawed once, and mice were injected intraperitoneally with Diphtheria toxin as previously described (1, 3). …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Materials and Methods:

The experiments with RABV/CTb injections were all performed on male Wistar rats (200-300 g), …

Surgical Procedures and Injections:

…A volume of 150 or 200 nL of a mixture of 1 part 1% CTb (low salt; List Biological Laboratories, 1% w/v in 0.2 M phosphate buffer (PB), pH 7.4) and 4 parts RABV (Ruigrok et al., 2008) was injected in every case at a depth of 300600 m below the surface. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Infection, Induction, and Scoring of EAE:

Female 8-week-old BALB/c mice were used throughout this study. …Each mouse was immediately thereafter, injected intraperitoneally and 48h later with 300 ng pertussis toxin (List Biological Laboratories, Campbell, CA, USA) in 100L 0.9% NaCl. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents and Drugs:

…Pertussis toxin was from List Biologicals…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAM and use of different adjuvants:

For the induction of EAM, we used the myocarditogenic peptide derived from the sequence of the murine cardiac myosin heavy chain (DSAF DVLS FTAE EKAG VYK for A/J mice and Ac-SLKLMATLFSTYASAD for BALB/c mice)… On day 0, all mice additionally received 500ng of pertussis toxin ip (List Biologicals, Campbell, CA). Individual experiments were conducted at least three times with five mice per group.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

Nave WT and CD73^-/- mice were immunized subcutaneously with 100g MOG(3555)…200 ng Pertussis toxin (List Biological Laboratories) was given intraperitoneally on day 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Animal models:

…Immunized mice also received 400ng of pertussis toxin (List Biological Laboratories, Campbell, CA) i.p. on days 0 and 2. The animals were scored daily for clinical signs of EAE.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Then, 80 µl of CTb neural tracer (1 mg/ml; List Biological Laboratories, Inc., Campbell, CA), in a solution containing 10% dimethylsulfoxide, was injected into the vitreous chamber of the left eye with a glass micropipette attached to a 100 µl Hamilton syringe. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

For retrograde tracing, …subunit B of cholera toxoid (CtB; 1% in distilled water; List Biological, Campbell, CA) or …were unilaterally injected into selected areas of the VTA or RMTg.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt