Bacterial Toxin Research Citations

EAE:

…containing heat-killed Mycobacterium tuber- culosis (Mtb, Difco, Detroit, MI) subcutaneously at four sites on the flanks followed by intraperitoneal injections of pertussis toxin (Ptx, List Biologicals, Campbell, CA), 75 ng on the day of immunization …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…CTB … 2% List Biological 103B …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

EAE:

…peptide in CFA containing 10 mg/mL heat-killed H37Ra, strain of Mycobacterium tuberculosis (Difco Laboratories). Animals received 250 ng pertussis toxin (List Biological Laboratories) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Neonatal CTB Injections for Anterograde Axonal Tracing:

Ferret kits aged P2 (n = 3) and P4 (n = 2) were deeply anesthetized and immobilized on a Kopf small animal stereotaxic apparatus. A borosilicate micropipette (20 m outer ) containing 2% CTB in PB pH 6.0 (List Biological Labs, Campbell, CA) was lowered into the LGN (1.2 mm posterior to Bregma; 1.11.2 mm lateral to midline; 4.85.5 mm under the pial surface). CTB was injected by iontophoresis applying positive current pulses 2 Amp, 7 on/off for 10 min. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

…Cholera toxin (CT) was purchased from List Biological Laboratories. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

LPS activation (control) is shown in the two bars to the right of the panel with an additional mock-coincubated control (100 ng ultrapure E. coli LPS [List Laboratories] was added to the cells for 3 h).

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

EAE:

… induced in 8 wk-old C57BL/6 mice by sc injection with 100 mg of myelin oligodendrocyte glycoprotein (MOG) 35-55 peptide (MEVG- WYRSPFSRVVHLYRNGK) emulsified with CFA on day 0 and iv in- jection with 400 ng of pertussis toxin (List Biological Laboratories …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mice and dietary intervention:

We used C57BL/6 mice or conditional AhR^CD11c and AhR^villin. …

Peanut extract (PE) (30 mg/ml) was prepared from peanuts from the Golden Peanut Plant (provided by Intersnack Nederland BV, The Netherlands) as described previously [20]. Peanut extracts were checked for protein content by BCA analysis (Pierce, Rockford, IL). Cholera toxin (CT) was purchased from List Biological Laboratories, Inc. (Campbell, CA).

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Immunohistochemistry:

Immunohistochemistry was performed as previously described…The primary antibodies used in these studies included rabbit anti-dsRed, which detects mCherry… and goat anti-CtB (1:10,000; product 703, List Biological Laboratories). …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Animals and Experimental Autoimmune Encephalomyelitis:

…Mice used in this study were included wildtype C57BL/6 (strain #000664) and GFAP-Cre mice (JAX strain #024098). To induce experimental autoimmune encephalomyelitis (EAE) wild-type C57BL/6 mice (68 weeks old) were immunized with a 1:1 ratio of myelin oligodendrocyte glycoprotein… The MOG-CFA emulsion was administered subcutaneously (s.c.) into the flanks of the hind-limbs (300 g/mouse). On days 0 and 2, pertussis toxin (PTX, List Biological) was injected intraperitoneally (i.p.) (500 ng/mouse). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)