Bacterial Toxin Research Citations

EAE Induction:

…Pertussis toxin was purchased from List Biological Laboratories (Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Surgical procedures.

All surgeries were performed under sterile conditions. Animals were induced with ketamine (0.1mg/kg i.m.), and maintained under isoflurane anesthesia (1.5% i.t.) for the duration of the surgery. They received buprenorphine analgesia prior to and following surgery. After incision of the overlying skin, the iliopsoas and semimembranosus muscles were exposed. Using a Hamilton syringe, we injected 4050l 0.5% cholera toxin subunit B (CTb; List Biological Lab,.CA; Cat# 703 in distilled water) into the iliopsoas and semimembranosus muscles. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Induction of EAE and clinical assessment:

Mice were immunized subcutaneously at the base of the tail with 50g MOG₃₅₅₅ peptide…200ng of pertussis toxin (List Biological Laboratories) was intraperitoneally injected on the same day and a second dose 2 days later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… This antibody stains a single point in two- dimensional immunoblots of extracts from rabbit cerebellum and kidney, at 28kD and pI 4.8, which is identical to calbindin D-28k (Celio et al., 1990). The anti-cholera toxin B subunit antibody (List Biological, …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Botulinum neurotoxins types A and B were purchased from List Biological Laboratories (Campbell, CA, USA).

…They were then incubated overnight in a primary antibody against CTB (anti-cholera B subunit made in goat, List Biological Laboratories, CA# 703, antibody RRID AB_10013220, Lot# 7032A6) diluted 1:33,000 in a blocking solution consisting of 0.4% triton and …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Antibody Determination:

Pertussis toxin, FHA, and Prn were purchased from List Biologicals and used to coat 96-well plates (Nunc) at 1 g/well overnight at 4C in PBS. …

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free). 

• Product #187 – Pertactin from B. pertussis (69 kDa Protein)

Product #170 is currently out of stock.  Large quantities may be obtained through Bulk Requests and Customer Orders.

Reagents and mouse strains:

…Pertussis toxin was purchased from List Biological Laboratories.

EAE induction following the fecal transfer:

EAE induction was performed as described previously… Briefly, on day 0, mice were immunized subcutaneously with 150 g of MOG₃₅₅₅ peptide emulsified in CFA in the two hind flanks and received 200 ng of pertussis toxin intraperitoneally 2 h later. Mice received another 400 ng of pertussis toxin on day 2 of the immunization…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

 …LPS was obtained from List Biological Laboratories (San Jose, CA, USA)…

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

EAE:

On the day of immunization and 48 h later, mice were administered intravenously with 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA, USA) in 200 l of PBS. Mice received daily intraperitoneal …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)