Citations

…Subsequently, ultra-pure LPS from E. coli O111:B4 (List Biological Laboratories) was added for further 30-min co-incubation with inhibitors. …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

…After BMP treatment the cells were exposed to 100 ng/ml LPS (E. coli O111:B4, List Biological Laboratories, Campbell, USA) for indicated time. …

• Product #201 – LPS from Escherichia coli O111:B4

– … and pertactin (Prn), and aluminum hydroxide adjuvant. Serology by ELISA Microtiter plates were coated with 2 g/ml DT, PT, FHA and Prn (List Biological, Campbell, CA) and washed with PBS/0.05% Tween20 (PBST). Wells were blocked with 1% BSA in PBST, and …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

• Product #187 – Pertactin from B. pertussis (69 kDa Protein)

Product #170 is currently out of stock.  Large quantities may be obtained through Bulk Requests and Customer Orders.

Animals:

…induced EAE. All mice also received 300 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) via intraperitoneal (i.p.) injection on the day of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…Then, 200 ng of pertussis toxin (List Biological Lab) were given intraperitoneally on days 0 and 2 post-immunization (p.i.). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE (experimental autoimmune encephalomyelitis):

To induce EAE, B10.PL mice were injected subcutaneously with a CFA emulsion containing 100 g Ac19 and 200 g of Mycobacterium tuberculosis H37Ra (Difco) divided among 3 sites on their backs. 1 and 3 day after the immunization, the mice were injected intraperitoneally with 100 ng of pertussis toxin (List Biological Laboratory) in PBS. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…Pertussis toxin was purchased from List Biological Laboratories. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mouse EAE induction and CNS lymphocyte isolation

Mice were immunized s.c. with 100 μl of emulsified IFA supplemented with 500 μg Mycobacterium tuberculosis H37Ra (DIFCO) and 100 μg of MOG35–55, and received an i.p. injection of 200 ng pertussis toxin (List Biological Laboratories) at the time of immunization as well as 48 h later. The mice were observed daily for clinical signs and scored …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Diphtheria toxin treatment:

Diphtheria toxin (DT) was administered to DTR mice, genetically engineered to express the human DTR selectively in hair cells (Tong et al. 2015). A single 25 g/kg dose of DT (List Biological Laboratories, no. 150) was delivered via intramuscular injection to 4-wk-old DTR mice. Within 6 days after DT injection, DTR mice lose all of their hair cells and are completely deaf (Tong et al. 2015). After DT injection, DTR mice were allowed to survive for 2 wk before tissue collection.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Tracer experiments

Cholera toxin subunit injections:

…Briefly, the retrograde tracer cholera toxin subunit (CTB; List Biological Laboratories, Campbell, CA) was targeted to the PVN (n = 10) and to the PIL (n = 15) in lactating mother rats at postpartum day 4. …Glass micropipettes of 15 to 20 µm internal diameter were filled with 0.25% CTB dissolved in PB and lowered to the following stereotaxic coordinates (35): anteroposterior (AP), 1.7 mm; lateral (L), 0.4 mm; ventral (V), 7.4 mm for the PVN; and AP, 5.2 mm; L, 2.6 mm; and V, 6.4 mm for the PIL. CTB was applied by iontophoresis using a constant current source…

Visualization of CTB:

Sections were pretreated in PB-containing 0.5% Triton X-100 and 3% bovine serum albumin for 1 hour. Sections were then incubated in goat anti-CTB antiserum (1:10,000; #703, List Biological Laboratories; …

Double immunolabeling of CTB and TIP39 or CB:

Every fourth free-floating section was first stained for TIP39 by using FITC-tyramide amplification fluorescent immunocytochemistry, as described earlier. Sections were then incubated overnight in goat anti-CTB or …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)