Bacterial Toxin Research Citations

Preparation of the MDS formulations:

RPA of anthrax (from List Biological) Loading: 0.8-1%

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

– … After resolving the desorbed Pfs25-EPA on a non-reducing tris-acetate SDS-PAGE gel, Western blot analysis was performed using anti-Pfs25 monoclonal antibodies 4B7 and 1G2 (LMIV) and an anti-Exotoxin A polyclonal antibody (List Biological Laboratories, Inc, Campbell …

• Product #760 – Anti-Exotoxin A (Goat) from Pseudomonas aeruginosa

a-cholera toxin B, List Biological Laboratories; Campbell, CA, goat, 1:2000

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Mice, BM chimeras and in vivo treatments:

…For experiments with Ccr2-depleter mice, mice were injected ip with 10 ng/g Diphtheria Toxin (DT) (List Biological Laboratories) in PBS. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… For the retrograde tracing, 0.2 µl 0.4% CTb (#104, List Biological Laboratories, Campbell, CA, USA) were injected at the following coordinates for the VS: + 2.2 mm anterior to Bregma; 0.75 mm lateral to the midline; and dorsoventral 4.25 mm below the dura. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

…As indicated, the pipette solution was added with ATPS (EMD Millipore Chemicals, Germany), the light chain of botulinum toxin C (List Biological Lab., USA), Pitstop 1 (Abcam, USA), an AP2-blocking peptide containing the DNF motif of …

• Product #625A –  Botulinum Neurotoxin Type C Light Chain, Recombinant

EAE Induction:

…Pertussis toxin was purchased from List Biological Laboratories (Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Surgical procedures.

All surgeries were performed under sterile conditions. Animals were induced with ketamine (0.1mg/kg i.m.), and maintained under isoflurane anesthesia (1.5% i.t.) for the duration of the surgery. They received buprenorphine analgesia prior to and following surgery. After incision of the overlying skin, the iliopsoas and semimembranosus muscles were exposed. Using a Hamilton syringe, we injected 4050l 0.5% cholera toxin subunit B (CTb; List Biological Lab,.CA; Cat# 703 in distilled water) into the iliopsoas and semimembranosus muscles. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Induction of EAE and clinical assessment:

Mice were immunized subcutaneously at the base of the tail with 50g MOG₃₅₅₅ peptide…200ng of pertussis toxin (List Biological Laboratories) was intraperitoneally injected on the same day and a second dose 2 days later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… This antibody stains a single point in two- dimensional immunoblots of extracts from rabbit cerebellum and kidney, at 28kD and pI 4.8, which is identical to calbindin D-28k (Celio et al., 1990). The anti-cholera toxin B subunit antibody (List Biological, …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)