Citations

Test samples were diluted and titrated on the plated cells in the presence or absence of LeTx (List Biological Laboratories, Campbell, CA, USA).

Lethal Toxin consistst of Lethal Factor and Protective Antigen:

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Serum samples from each mouse were then added to the monolayer of macrophages in increasing concentrations and serum and cells were co-cultured with PA (0.5 g/mL) and lethal factor (LF, 0.5 g/mL, List Biological Laboratories, Inc, Campbell, CA).

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

From the same subjects, whole blood was stimulated with ultrapure LPS from Salmonella minnesota R595 (List Biological Laboratories, Inc., Campbell, CA).

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

 Induction of EAE and Therapeutic Study:

…In addition, 200 ng of pertussis toxin (List Biological Laboratories Inc., Campbell, CA) was injected (100 µL) intraperitoneally on the day of immunization (day 0) and 2 days post-immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…the dura was punctured and two 200 nl injections of cholera toxin B subunit (CTB; List Biological Laboratories, USA; .5 %) were made on each side at 0.75 mm lateral to the midline and 1 mm deep to the surface, co-ordinates that correspond to the interomediolateral column (IML) in the adult rat (Turner et al., 2013)….

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Enzyme-linked immunosorbent assay (ELISA):

Samples were grown overnight in LB-L media, then subcultured 1:100 in LB-L media and grown for 8 h at 37 C and 225 rpm. The cultures were pelleted by one centrifugation step of 2272g for 10 min and the supernatant was passed through a 0.45 m filter. The wells of a 96-well microtiter plate (Santa Cruz Biotechnology, Inc., part# sc-204463) was coated with 100 L of a solution of 4 g mL¹ protective antigen (PA) of the anthrax toxin (List Biological Laboratories, part# 171E) in 5 mM HEPES, 50 mM NaCl, pH 7.5 covered at 4 C overnight. Liquid was removed by inversion and wells were incubated with a 200 L blocking solution (200 L 2% milk, 0.05% TBST) at room temperature for 1 h. …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Vaccines, TLR Agonists, and Multi-Analyte Assays:

Licensed vaccines were commercially obtained. Ultrapure LPS (Salmonella minnesota R595, List Biological Laboratories, Campbell, CA, USA) was used at 100ng/ml.

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

… PTX was obtained from List Biological Laboratories (Campbell, CA, USA) …

Coimmunoprecipitation:

Coimmunoprecipitation experiments were carried out as described before³⁵. Briefly, HEK293T cells were transiently transfected by using the calcium phosphate method. After incubation for 24h, the cells were stimulated with Ang II (1M) for the time indicated and harvested thereafter. For inhibitor experiments, cells were pretreated with the inhibitor or its control (Candesartan 100nM, PP2 10M, PTX 200ng/ml, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… The following inhibitors were used: Pertussis toxin (PTX) (List Biological Laboratories, 100 ng/ml), …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

CNS Autoimmune Disease Induction and Analysis:

…Mice received 200 ng B. pertussis toxin (Ptx) (List Biological) by i.p. injection on days 0 and 2. …At day 0 and 2, mice received Ptx. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)