Bacterial Toxin Research Citations

… A total of 3 µl of 1% Cholera toxin subunit B (CTB; List Biological Laboratories, Inc., CA, USA) was injected into the sciatic nerve using a thin glass needle and the skin was closed using single silk 4.0 sutures. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Antibody assessment:

Recombinant proteins (rPA and rLF) were …or purchased from List Biological Laboratories (Campbell, CA). Antibody titers against PA were determined by enzyme-linked immunosorbent assay (ELISA). Plates were coated with rPA at 2.0ng/L in a volume of 50L/well and incubated overnight at 4C. Plates were then washed three times with 1wash solution (KPL, Gaithersburg, MD), and blocked with 1% bovine serum albumin (KPL) containing 1% non-fat dry milk for 1h at 37C. …

The lethal toxin-neutralizing assay (TNA) titer was determined as described³⁴ except J774A.1 murine macrophage cells were used. Briefly, the serially diluted sera were pre-incubated with toxin mixture (PA and LF at 5:4 ratio) at 37C for 30min before adding to J774A.1 cells in 96-well plates. …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… and pertussis toxin was purchased from List Biological Laboratories, Inc. (Campbell, California).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… At the same time, and again after three days, each mouse received 300 ng pertussis toxin (List Biological Laboratories, Inc., CA, USA) in PBS i/v via the tail vein. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Transganglionic tracers,…and Cholera Toxin Subunit B (CTB, List Biological Laboratories, Campbell, CA), … The anti-CTB antibody (703, List Biological Laboratories, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… A solution of 1% cholera toxin B subunit (CTB; List Laboratories, … Sections were incubated with antibodies against CTB made in goat (1:12,000, List laboratories), followed by …

Author did not specify which CTB was utilized.  List Labs provides Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae) and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Ultra-pure LPS (Escherichia coli 0111:B4) was obtained from List Biologicals (Campbell, CA, USA). … Ultra-pure LPS (E. coli0111:B4) from List Biologicals was dissolved in DNase-/RNase-free sterile normal saline and intraperitoneally injected (5 mg/kg) (44, 46). …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Induction of EAE and Treatment:

…Mice were immunized s.c. in the flank with 200g MOG_35-55 emulsified in CFA supplemented with 300g Mycobacterium tuberculosis (Mt) H37RA (Difco). Pertussis Toxin (PTX, List Biological Laboratories, CA, USA) was injected i.v. at the time of immunization and 48h later. In some experiments, animals were sacrificed prior to onset of clinical symptoms in order to analyze the lymph node response. EAE was scored on a scale of 06: 0, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction:

EAE was actively induced by subcutaneously injecting 250 L MOG₃₅₅₅ peptide… Additionally, the animals received intraperitoneally (i.p.) 200 ng pertussis toxin (List Biological Laboratories) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Tracer Injections:

… For retrograde tracing, a low salt formulation of Cholera Toxin Subunit b (CTb; No. 104, List Biological
Laboratories, Campbell, CA) was applied via iontophoretic injection (Ericson & Blomqvist, 1988). …

Immunohistochemistry:

… For visualization of CTb, the tissues were washed three times for ten minutes in PB and then
incubated overnight at 4°C in a primary antibody solution of goat antiserum against CTb (List Biological
Laboratories, Campbell, CA) and 0.3% Triton PB. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)