Citations

EAE Induction:

Female SJL mice (612 wk old) were obtained from Jackson Laboratories. EAE was induced by s.c. injecting 200 µg proteolipid protein (PLP) peptide …Two hundred nanograms of pertussis toxin (PTx) (List Biological Laboratories) dissolved in 200 µL PBS was administered i.p. on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

PA₆₃ was purchased from List Biological Laboratories, Inc., (Campbell, CA, USA). …

Channel Reconstitution into Planar Lipid Bilayers:

To form solvent-free planar lipid bilayers with the lipid monolayer opposition technique [81], we used …Single channels were formed by adding 0.5 to 1 L of 20 gmL¹ solution of PA₆₃ to the 1.5 mL aqueous phase in the cis-half of the bilayer chamber. Under this protocol, PA₆₃ channel insertions were always directional, as judged by channel conductance asymmetry in the applied transmembrane voltage. …

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

Table 3.2:

Primary antibodies for in vivo growth in SC sections

Primary antibody Made in  Producer Dilution used

Anti CTB Gt List Biological 1:80000

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Reagents:

LPS was obtained from List Biological Laboratories (San Jose, CA, USA). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Materials:

…while ETA, DT, and CT were purchased from List Biologicals (Campbell, CA). …

CT toxicity assay:

CHO-K1 cells (ATCC #CCL-61) were co-incubated with a combination of CT and grape compound for 18 h before cAMP levels were quantified as previously described…

Vero-d2EGFP toxicity assay:

Ricin, ETA, DT, and ST1/ST2 intoxication assays were peformed with Vero-d2EGFP cells as previously described…

Author did not specify which Cholera toxin was utilized.  List Labs provides Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae and Product #101 – Cholera Toxin from Vibrio cholerae.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
• Product #160 – Exotoxin A from Pseudomonas aeruginosa

Adoptive transfer and infection:

Spleens were collected from naive CD45.1+ gBT-I TCR transgenic mice, and CD8 T cells were magnetically purified by CD8 microbeads or …For experiments with DT depletion, animals were injected intraperitoneally with 0.5g of DT (List Biological) 1 day before WT HSV-2 challenge¹³.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Thus, ultra-pure LPS, available from select companies (eg, Invivogen or List Biological Laboratories), will not cause inflammation in the absence of TLR4.

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Primary mouse macrophage culture and treatment:

WT and miR-223^-/- mice were obtained from Jackson Laboratories. …Treatments consisted of unstimulated controls, LPS (100 ng/ml, LCD25; List Biological Laboratories) or a combination of LPS (100 ng/mL) & IFN (100 ng/ml) for 24 hours.

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Protein preparation and purication:

The PTx holotoxin (PTx)…were obtained from List Biological Laboratories.

PTx was used utilized in: PTx binding assays; Murine PTx leukocytosis study;  In vitro PTx neutralization assay

Author specified that List Labs Product #180 was utilized in their research.

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… to as OVA- A594) (Invitrogen, #O34783) was added to the wells at 5 g/ml formulated with the indicated TLR ligand as adjuvant, ie 100 ng/ml E. coli strain O111:B4 LPS (LIST Biological Laboratories, Page 14. 14 USA), …

• Product #201 – LPS from Escherichia coli O111:B4