Bacterial Toxin Research Citations

Macrophage culture:

…BMDMs were primed for 4h with 600ngml¹ E. coli 0111:B4 LPS… and then transfected with poly(dA:dT)…, lethal toxin (500ngml¹, #172D and #171D mixed at a 50/50 ratio, List Labs) or flagellin…

• Product #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Immunization and challenge of mice:

Ninety-one 6-week-old female BALB/c mice (Charles River Laboratories, MA) were randomly divided into seven groups of 13 mice and used for immunization. In protein administration groups, mice were immunized intranasally with 10 μg of rec47 with or without 10 μg of heat-labile enterotoxin B subunit (LTB; Sigma Aldrich Co., Saint Louis, MO) from E. coli or 10 μg of cholera toxin (CT; List Biological Laboratories Inc., CA, USA) adjuvants. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE:

… At the same time, mice were also administered 250 ng of pertussis toxin (List Biological Laboratories) ip and again 2 d later. Clinical disease was monitored daily and scored …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Evaluation of anti-CLEC12A antibody in EAE immunized mice:

Progressive EAE was induced in female C57BL/6 mice (68 weeks old) and the CLEC12A/ mice as described previously⁶⁰⁶². Briefly, 100L of 150g myelin oligodendrocyte glycoprotein (MOG₃₅₅₅) (PolyPeptide Laboratories) peptide emulsified in complete Freunds adjuvant (Difco) containing 4mg/ml killed Mycobacterium tuberculosis (strain H37Ra; Difco) was injected subcuataneously. Following immunization, 200ng of pertussis toxin (List Labs) was injected i.p. into mice on Day 0, followed by a 400ng pertussis toxin i.p. injection on Day 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Macrophage Culture And Activation:

…After overnight culture, cells were washed in PBS and incubated with 2 ng/ml LPS (List Biological Inc., Campbell, CA, USA) or control in DMEM + 1% FBS for 4h before harvest. Viability of cultured macrophages was determined for some strains by staining with 2 M calcein AM …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Proteins:

…Protective antigen (PA) from Bacillus anthracis (List Biological Labs, Campbell, CA, USA) was used in the assay at a final concentration of 3 g/ml. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Preparation of the MDS formulations:

RPA of anthrax (from List Biological) Loading: 0.8-1%

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

– … After resolving the desorbed Pfs25-EPA on a non-reducing tris-acetate SDS-PAGE gel, Western blot analysis was performed using anti-Pfs25 monoclonal antibodies 4B7 and 1G2 (LMIV) and an anti-Exotoxin A polyclonal antibody (List Biological Laboratories, Inc, Campbell …

• Product #760 – Anti-Exotoxin A (Goat) from Pseudomonas aeruginosa

a-cholera toxin B, List Biological Laboratories; Campbell, CA, goat, 1:2000

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Mice, BM chimeras and in vivo treatments:

…For experiments with Ccr2-depleter mice, mice were injected ip with 10 ng/g Diphtheria Toxin (DT) (List Biological Laboratories) in PBS. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae