Bacterial Toxin Research Citations

…Pertussis Toxin (PT) was purchased from List Biological Laboratories, Inc. (Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction

Mice were subcutaneously injected in the hind limb with emulsified equal volume of MOG_35-55 peptide…then intraperitoneally injected with pertussis toxin (PTX, 200ng/mouse, List Biological Laboratories, Campbell, CA) on the day of immunization and 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

The primary antibodies used are anti-CTb goat serum (#703, List Biological Laboratories), …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE induction and assessment:

EAE was induced in 8- to 12-week-old female WT and KO mice⁴². Briefly, mice were immunized with 300g MOG₃₅₅₅ peptide (Biomatik, Wilmington, DE, USA) emulsified in complete Freund’s adjuvant, followed by i.p. injection of 400ng pertussis toxin (List Biological Laboratories, Campbell, CA, USA) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

In the sensitization experiments, six-week-old mice (n=5 per group) were administered by intragastric gavage, during three consecutive days on the first week and once per week during the following 5 weeks, the antigen (1 mg of either OVA, OM or LYS, a mixture of 1 mg of each of the three proteins, 5 mg of EW or 5 mg of either OA or OP) plus 10 µg of CT (List Biologicals, Campbell, USA). Sham-sensitized mice only received 10 µg of CT.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Labeling from retinal projections

Unilateral injections:

Six animals (three S. cyanus and three C. talarum) were anesthetized as previously mentioned, and given intraocular injections (8µL, left eye) of 0.5% Cholera toxin subunit B (CTB, List Biological Laboratories) mixed with 1% DMSO.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

To assess potential recall responses, PCLS were treated on day 2 of cultivation with either …or tetanus toxoid (List Biologicals Inc, Campbell, California; used at 5 g/mL).

• Product #191 – Tetanus Toxoid from Clostridium tetani

… was then cleared and 4-5 l of CT- (1% solution, w/v in distilled water; MW 11.5 kDa, List Biological Laboratories, CA … Primary antibodies included: CT- (1:2000, polyclonal goat anti-Cholera Toxin subunit, List Laboratories, Product# 703), …

Author did not specify which CTB was utilized.  List Labs provides Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae) and Product #104 (Cholera Toxin B Subunit (Choleragenoid)
from Vibrio cholerae in Low Salt).

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… These sections were incubated at room temperature sequentially with 0.5 g/ml goat anti-CTB antibody (1) (List Biological, Campbell, CA) overnight and then …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

…Subsequently, ultra-pure LPS from E. coli O111:B4 (List Biological Laboratories) was added for further 30-min co-incubation with inhibitors. …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4