Citations

Surgical Procedures

C5 Unilateral Spinal Cord Contusion

… Three days prior to being euthanized rats in Experiment 2 were anesthetized with XAK, and an axial incision to the ipsilesional (right) forelimb exposed the median and ulnar nerves,26 and the perineurium was removed. A 5-µL Hamilton syringe fitted with a custom 30 gauge needle (#7803-07, Hamilton Company, Reno, NV) was inserted into each nerve, and 2.0 µL of cholera toxin β subunit (CTB; 10 µg/µL; Product #104, List Biological Laboratories, Inc, Campbell, CA) was injected to label myelinated sensory afferents. Rats received 5 cc of LRS and cefazolin subcutaneously following surgery.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

MC3R Expression in the VTA:

A cholera toxin B (CTB)-based retrograde tracing strategy was used to study whether Arc POMC neurons projected to the VTA. To label perikarya of neurons in the Arc nucleus projecting to the VTA, the rats (n=6) were treated with CTB (0.5% solution; #103, List Biological Laboratories, Campbell, CA) via unilateral iontophoresis (5A, 7s onoff) into the VTA for 20min.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

EAE immunization and SU5416 treatment:

To induce EAE, 7-weeks old C57BL/6J female mice were injected subcutaneously in the flank and at the tail base with 200 µg of myelin oligodendrocyte glycoprotein 35 to 55 (MOG 3555) peptide emulsified in complete Freunds adjuvant (BD Biosciences, San Jose, CA) supplemented with 600 µg of mycobacterium tuberculosis (strain H37Ra; BD Biosciences). Two intraperitoneal injections of 400 ng pertussis toxin (List Biological Laboratories, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…Sections were then incubated with cholera toxin B subunit-biotin conjugate (List Biological Laboratories, Campbell, CA, USA) diluted at 0.5 µg/ml with PBS plus 3% BSA, at room temperature for 60 min. …

• Product #112 – Cholera Toxin B Subunit Biotin Conjugate

Reagents:

Escherichia coli 0111:B4 LPS was purchased from List Biological Laboratories (Campbell, CA). …

ELISA and multiplex cytokine assays:

After stimulation with LPS at the indicated time points, cell supernatants were collected and analyzed using ELISA Kits (eBioscience) or Bioplex (Bio-Rad) according to the manufacturers protocols. 

• Product #201 – LPS from Escherichia coli O111:B4

For experiments where cells were treated with pertussis toxin (PTX), the cells were plated as normal and PTX (200 ng/mL final concentration, List Biological Laboratories, Campbell, California) was added to the wells immediately afterward. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

RABV Injection in the Inferior Olive:

The inferior olive was injected in 11 rats. …

The injectate consisted of a mixture of one part cholera toxin, b-subunit (CTb: low salt: List Biological Laboratories, 1% w/v in 0.2 M phosphate buffer, pH 7.4) and four parts RABV. RABV consisted of a cell culture supernatant in minimal essential medium titrating 4 10⁷ PFU/ml.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

C. difficile TcdA immunoblotting:

C. difficile TcdA (List Biologicals, Campbell, California, USA) binding to PSGL-1/mIgG2b was assessed by incubating the nitrocellulose membrane with 1 g/mL TcdA followed by anti-C. difficile Toxin A chicken IgY (List Biologicals) diluted 1:1,000 and…

• Product #152C – Toxin A from Clostridium difficile

Induction and treatment of EAE:

Mice were immunized subcutaneously with 300 µg MOG₃₅₅₅ peptide….Pertussis toxin (200ng, List Biological Laboratories, Campbell, CA, USA) in PBS was administered i.v. on days 0 and 2 post immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental Autoimmune Encephalomyelitis (EAE):

Induction of EAE was performed according to the protocol of Miller and Karpus (2007) … Female mice, at 8-10 weeks old, were subcutaneously injected in two sites (100 µL by site) adjacent to the tail with the emulsion. Pertussis toxin (200ng) (List Biological Laboratories Inc., Campbell, CA, USA) was administered intraperitoneally on the same day of immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)