Bacterial Toxin Research Citations

…Cholera toxin (List Biological Laboratory, No. 100B) ! CAUTION Cholera toxin is potentially harmful. Wear gloves and handle it carefully in a biosafety cabinet. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

…Briefly, the sections were incubated at room temperature with a mixture of goat anti-CTb Ig (1:2,000; List Biological, Campbell, CA, USA), …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Cholera Toxin B Subunit (CtB), Goat, Polyclonal, Purified CtB. isolated from Vibrio. cholerae. List Biological. Laboratories, Campbell, CA, USA. Catalogue number 703. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

– … The cells were stimulated with 10 g/L ultra-pure, endotoxin-free LPS (List Biological Laboratories, Inc, Campbell, CA, USA) per well. An equal volume of sterile endotoxin-free water was added to the negative control wells. …

Author indicated that the following List Labs product was utilized:

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

EAE:

EAE was induced in male and female B6 and PWD mice as follows. Mice were injected subcutaneously with 0.1 ml of emulsion containing 2.5 mg of MSCH in PBS and 50% CFA (Sigma, USA) on day 0 and day 7. CFA was supplemented with 4 mg/ml Mycobacterium tuberculosis H37Ra (Difco, USA). On day 0 and day 2 mice also received an i.p. injection of 200 ng pertussis toxin (List Laboratories, USA) as an ancillary adjuvant. Starting on day 10, mice were scored visually as previously described …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Mice received 100 ng pertussis toxin (List Biological Laboratories, Campbell CA) ip on days 0 and 2. Disease severity was evaluated using the following scale: 1: flaccid tail; 2: impaired righting reflex and hindlimb weakness; 3: partial hindlimb paralysis; 4: complete …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…Pertussis toxin (product no. 180) was purchased from List Biological laboratories, INC. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Experimental Induction of Autoimmune Encephalomyelitis With Intracranial or Intramuscular hPSC Delivery:

Mice were immunized by subcutaneous injection of 300 µg of myelin oligodendrocyte glycoprotein (MOG) peptide corresponding to amino acids 3555 (MOG3555), diluted in normal saline (0.9% NaCl), and emulsified with complete Freund’s adjuvant. Bordetella pertussis toxin (300 ng; List Biological Laboratories, Inc., Campbell, CA, http://www.listlabstage.wpengine.com) was diluted in 0.2 ml of normal saline and injected i.p. immediately after MOG immunization and 2 days later. Clinical signs of EAE typically appeared 1012 days after immunization, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…On the day of, and 2 d after, immunization, the mice were also inoculated i.p. with 200 ng of pertussis toxin (List Biological Laboratories). Adoptive transfer (passive) EAE was induced by injecting recipient mice with 5.0 106 polarized MOG-specific Th17 cells.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE

EAE was induced by a subcutaneous immunization with 200 μg of MOG peptide (35-55) (Sangon Biotech Co.) and 1 mg of a heat-killed H37Ra strain of Mycobacterium tuberculosis (Difo) emulsified in complete Freund’s adjuvant injected into the back and head region. This was followed by an injection of pertussis toxin (List Biological Laboratories) at a dose of 200 μg/mouse in the tail vein. Forty-eight hours later, pertussis toxin was administered again. Disease severity was scored as previously described 15.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)