Bacterial Toxin Research Citations

Reagents:

… Botulinum toxins A and B were purchased from List Biologicals Laboratories, Campbell, CA. …

Study of the paralytic effect of BoNTs on detrusor contractions:

After intensive washings and after at least 20 min of stable contractions, BoNTs were added to the baths, with final concentrations of 0.1 to 5 nmol/L. Each strip was exposed to only one concentration of BoNT. Amplitude of contractions was measured until 90% of the signal was abolished, or for 4 h if this value was not reached (for low concentrations). …

ELISA:

BMDMs were primed with ultra-pure LPS (3 ng/mL; List Biological Lab) for 4 hr and then stimulated with particles for 4 hr at 37C. …

Cell Death Assay:

LPS (3 ng/mL)-primed BMDMs were stimulated with the indicated particles for 4 hr at 37C. …

Immunoblot Analysis:

…In brief, BMDMs (1  10⁶/well) were seeded in 6-well plates and were cultured overnight. Cells were primed with LPS (3 ng/mL) for 4 hr at 37C. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Induction and Scoring of EAE:

EAE was induced as in prior studies… In addition, each animal received 200ng pertussis toxin (List Biological, Campbell, CA, USA) in 0.1ml PBS by i.p. injection at 0h and 48h post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Sorting and activation of BMBs and SPBs:

…After sorting, 0.510⁵ BMBs and SPBs were activated with IgE…or CT (100ng/ml, List Biological Laboratories, Campbell, CA) for 2hours. …

Immunization with CTO:

For immunization, Mcpt8-cre-YFP mice and Mcpt8-creROSA DT mice were challenged intranasally with 40g of OVA_323-339 peptide with 2µg of CT (List Biological laboratories) in PBS³². …

Experimental autoimmune encephalomyelitis:

…All mice also received 200ng pertussis toxin (List Biological Laboratories) intraperitoneally on day 0 and 2. Mice were measured clinical scores for EAE according to the following scale: …

Adoptive transfer of BMBs:

After BM culture, WT and IL-6 deficient CD49b(DX5)⁺/c-kit basophils were isolated using a FACSAria II. Isolated basophils were injected intravenously into IL-6 deficient mice. The next day, mice were challenged intranasally with 40g of OVA peptides with 2µg of CT. Inflamed lungs and draining LNs were collected after 7 days.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

ELISA assays:

An ELISA assay was used to measure the serum IgG titer of immunized animals for TcdA and TcdB. …96-well plates were coated with 50 ng per well TcdA (List Biological Laboratories, Campbell, CA), 25 ng per well TcdB (List Biological Laboratories, Campbell, CA), 250 ng per well 1mCDTa_ec or 250 ng per well proCDTb_ec in PBS and incubated overnight at 4C. …

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile

Immunization of Mice:

…Mice received 200ng/mouse pertussis toxin (List Biological Laboratories Inc., Campbell, CA, USA) i.p. on the day of immunization and 2 days thereafter. Clinical signs of disease were scored daily according to a standard scoring system (0, no clinical signs; 1, loss of tail tone; 2, hind limb weakness; 3, complete hind limb paralysis; 4, hind limb and forelimb paralysis; and 5, moribund or dead). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…were incubated with two-fold serially diluted pig serum or colostrum samples (diluted from 1:200 to 1:25,600) to detect anti-STa IgG or IgA antibodies; wells of Immulon 2HB microtiter plates (Thermo Scientific, Rochester, NY) coated with LT [List Biological Laboratories …

Lethal Toxin consists of Lethal Factor & Protective Antigen:

Author did not indicate which specific Lethal Factor was utilized.  List Labs provides:

• Product #172 – Anthrax Lethal Factor (LF),Recombinant from B. anthracis
• Product #169 – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

List Labs provides the following Protective Antigen:

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

…or cholera toxin (CT; List Biological Laboratories, CA) was mixed with the vaccine antigen immediately before injection. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Induction of EAE and Administration of LH2171:

Female SJL/J mice (5 weeks old) were used to assess the immunosuppressive effect of LH2171 on EAE. …

… in addition to two intraperitoneal injections of 400 ng of pertussis toxin (List Laboratories, Campbell, CA, United States) on days 0 and 2. EAE mice were divided into two groups: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…in 96-well flat-bottomed tissue culture plates at 2.5×10 4 cells/well in 50 L media and incubated for 16-19 h at 37 C with 5% CO 2 . pp-dPA83 formulations were titrated on the plated cells in the presence of a constant concentration of LF (List Biological Laboratories …

Author did not specify which Lethal Factor was utilized.  List Labs provides the following Lethal Factor:

• Product #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169 – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence