Bacterial Toxin Research Citations

Pertussis-Toxin List Biological Laboratories, Campbell,CA, USA

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Peptide priming:

…On days 0 and 2, 300ng of pertussis toxin (Ptx, List Biological Labratories, Campbell CA, USA) was injected intraperionteallly in all immunizations to compare both the self and foreign immune responses.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Isolation and cultivation of OECs derived from luc tg rats:

…The composition of KCM is as follows: DMEM high-glucose medium (Gibco), Nutrient Mixture F-12 (Gibco), 10% fetal bovine serum (Japan Bio Serum), 400ng/mL hydrocortisone succinate (Wako), 2nM 3,3,5- triiodo-l-thyronine sodium salt (MP Biomedicals), 1nM cholera toxin (List Biological Laboratory), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Cell culture and immunoblotting:

MCF10A and MCF10DCIS.com [37] cells were cultured in Hams F12/DMEM (Cellgro) supplemented with …100ng/ml cholera toxin (List Biological Laboratories), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… The amount of immunized antigen was as follows: J8-VLPs (50 g) with or without cholera toxin (CT; 1 µg; List Biological Laboratory, Campbell, CA); FD-J8-VLPs (50 µg) with or without CT (1 µg); …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

…Clostridium difficile Toxin A List Biological Labs 152C Positive control for Vero Cell Assay…

• Product #152C – Toxin A from Clostridium difficile

Reagents:

Purified LPS from Escherichia coli was obtained from List Biological Laboratory Inc. (Campbell, CA). …

Analysis of inflammasome activation:

This was performed as previously described²⁸. Briefly, BMDMs were primed with LPS for 4hrs. To exclude the effect of CAPE on LPS, CAPE was added after washing out the LPS with phosphate-buffered saline(PBS). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Materials:

…Pertussis toxin was acquired from List Biological Laboratories Inc. (Campbell, CA). …

Measurement of Intracellular cAMP Levels in Intact Cells:

…To determine if modulation of adenylyl cyclase activity by SERMs was mediated through CB2 receptors coupling to G_i/o proteins, additional cAMP assays were conducted following overnight treatment with pertussis toxin as described elsewhere [28]. Briefly, CHO-hCB2 cells were seeded into 24-well plates as described above in normal culture media containing 100 ng/ml of pertussis toxin and incubated overnight at 37°C. Pertussis toxin-treated media was removed and measurement of intracellular [³H]cAMP levels following indicated SERM treatments was conducted as described above.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…CT (List Biological Laboratory, Campbell, CA, USA) was used as a nasal adjuvant.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE Induction.

Induction of active EAE was performed as previously described (37). Briefly, mice received a total of 200 µg MOG₃₅₅₅, 200 µg complete Freunds adjuvant (CFA), containing 4 mg/mL Mycobacterium tuberculosis (H37RA, BD Biosciences), and 200 ng pertussis toxin (List Biological Laboratories). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)