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February 7, 2024
Science Translational Medicine
Koenig, JFE;Knudsen, NPH;Phelps, A;Bruton, K;Hoof, I;Lund, G;Libera, DD;Lund, A;Christensen, LH;Glass, DR;Walker, TD;Fang, A;Waserman, S;Jordana, M;Andersen, PS;
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
… Unless otherwise specified, mice were sensitized by a single intraperitoneal injection of 250 µg of OVA (Sigma-Aldrich, A5478) and 5 µg of CT (List Labs; 100B) in phosphate-buffered saline (PBS; Sigma-Aldrich, P4417-100TAB). Epicutaneous sensitization was performed by applying 200 µg of OVA after shaving or tape stripping the lower back …
• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
February 6, 2024
Frontiers In Immunology
Yang, N;Srivastava, K;Chen, Y;Li, H;Maskey, A;Yoo, P;Liu, X;Tiwari, RK;Geliebter, J;Nowak-Wegrzyn, A;Zhan, J;Li, XM;
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
February 5, 2024
Biorxiv
Kim, J;Kim, D;Kim, D;Lee, S;Jang, W;Lim, D;
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
Cell Culture
MCF-10A cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM)- F12 (Welgene, LM002-04) supplemented with 5% horse serum (Invitrogen, 26050-088), epidermal growth factor (Peprotech, AF-100-15) at 20 ng/ml, insulin (Gibco, A11382II) at 10 μg/ml, hydrocortisone (Sigma, H0888) at 0.5 μg/ml, and cholera toxin (List Biological Laboratories, 100B) at 100 ng/ml. HEK293T, MDCK, HFE-145 (gastric epithelial) cells and PANC-1 (pancreatic adenocarcinoma) cells were cultured in DMEM medium supplemented with 10% fetal bovine serum (FBS) (Gibco, 12483020). AGS, MKN-45 (both from gastric adenocarcinoma), and BCPAP (thyroid carcinoma) were cultured in RPMI medium supplemented with 10% FBS.
• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
February 5, 2024
Journal Of Immunology
Kagohashi, K;Sasaki, Y;Ozawa, K;Tsuchiya, T;Kawahara, S;Saitoh, K;Ichii, M;Toda, J;Harada, Y;Kubo, M;Kitai, Y;Muromoto, R;Oritani, K;Kashiwakura, JI;Matsuda, T;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
EAE
… The mice were s.c. immunized with 100 µg of MOG (Sigma-Aldrich) emulsified in CFA (BD, Sparks, MD) plus 500 µg of Mycobacterium tuberculosis H37Ra (BD). At the times of immunization and 2 d later, the mice were i.v. injected with 400 ng of pertussis toxin (List Biological Laboratories). Disease severity of EAE was monitored using the criteria as previously described (26). …
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
February 3, 2024
Nature Communications
Wang, XF;Vigouroux, R;Syonov, M;Baglaenko, Y;Nikolakopoulou, AM;Ringuette, D;Rus, H;DiStefano, PV;Dufour, S;Shabanzadeh, AP;Lee, S;Mueller, BK;Charish, J;Harada, H;Fish, JE;Wither, J;Wälchli, T;Cloutier, JF;Zlokovic, BV;Carlen, PL;Monnier, PP;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
Induction and scoring of Experimental Autoimmune Encephalomyelitis (EAE) mice
Female mice were subcutaneously injected with an emulsion of 50 μg myelin oligodendrocyte glycoprotein (MOG) amino acids 35-55 (Sheldon Biotech, Montreal, QC) peptide in incomplete Freund’s adjuvant (Sigma) supplemented with 1 mg of mycobacterium tuberculosis (CFA) (Difco). 400 ng of pertussis toxin (List Biologicals) was administered intraperitoneally on days 0 and 2 post-immunization. For ligand treatment, 40 μg of purified protein was administered intravenously once every three days for the duration of the experiments. …
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
February 2, 2024
Biorxiv : The Preprint Server For Biology
Yu, H;Nishio, H;Barbi, J;Mitchell-Flack, M;Vignali, PDA;Zheng, Y;Lebid, A;Chang, KY;Fu, J;Higgins, M;Huang, CT;Zhang, X;Li, Z;Blosser, L;Tam, A;Drake, CG;Pardoll, DM;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
EAE induction
EAE was induced in mice by subcutaneous injection of 200 μg MOG35–55 peptide with 500 μg M. tuberculosis strain H37Ra (Difco) emulsified in incomplete Freund Adjuvant oil in 200ul volume into the flanks at two different sites. In addition, the mice received 400 ng pertussis toxin (PTX; List Biological Laboratories) i.p. at the time of immunization and 48 h later. …
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
February 1, 2024
Cell Reports Medicine
Chaudhuri, S;Weinberg, S;Wang, D;Yalom, L;Montauti, E;Iyer, R;Tang, A;Torres Acosta, M;Shen, J;Mani, N;Wang, S;Liu, K;Lu, W;Bui, T;Manzanares, L;Dehghani, Z;Wai, C;Gao, B;Wei, J;Yue, F;Cui, W;Singer, B;Sumagin, R;Zhang, Y;Fang, D;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
Induced experimental autoimmune encephalomyelitis (EAE) model
Per mouse, 200ug of MOG20,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54 peptide (EZ Biolabs #cp7203) emulsified in Freund’s Complete Adjuvant (Santa Cruz Biotechnologies #sc-24018). Mice were then subcutaneously injected with 150ul of the emulsification with 75ul each on the right and left flanks. Mice were then intraperitoneally injected with 200ng of pertussis toxin (List Biologicals #180) and then once again two days later. Mice were assessed daily for clinical signs of EAE. …
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
February 1, 2024
Nano Today
He, Z;Lai, J;Wang, H;He, Y;Zhang, C;Gao, L;Huang, H;Zheng, L;Hao, J;Gao, X;Gao, F;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
… About 500 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) was injected intraperitoneally on day 0 and 2. Mice were monitored for body weight and EAE onset daily. …
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
February 1, 2024
Immunity
Lan, Y;Zhang, X;Liu, S;Guo, C;Jin, Y;Li, H;Wang, L;Zhao, J;Hao, Y;Li, Z;Liu, Z;Ginhoux, F;Xie, Q;Xu, H;Jia, J;He, D;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
… Pertussis toxin (100 ng/mouse, List Biological Laboratories) was administered intravenously into the tail vein on day 0 and day 2 after immunization. The disease course was monitored daily, and mice were assigned a clinical score as …
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
January 31, 2024
Microorganisms
Tessier, E;Cheutin, L;Garnier, A;Vigne, C;Tournier, JN;Rougeaux, C;
Product: Anti-Edema Factor from B. anthracis (Goat)
… goat anti-EF (773L, List Labs, Campbell, CA, USA), …
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