Bacterial Toxin Research Citations

nthesized by Shenggong Biotech Shanghai Co., Ltd. Complete Freund’s adjuvant (CFA)(SLBW1457) was purchased from Sigma?Aldrich (St. Louis, USA). Pertussis toxin (PTX) (180243A1) was purchased from List Biological Laboratories (Campbell, USA). HRP goat anti-rabbit antibody (GB23303) was obtained from Servicebio Biotechnology Co., Ltd. Anti-fluorescence quenching solution (P0126) and haematoxylin … nthesized by Shenggong Biotech Shanghai Co., Ltd. Complete Freund’s adjuvant (CFA)(SLBW1457) was purchased from Sigma?Aldrich (St. Louis, USA). Pertussis toxin (PTX) (180243A1) was purchased from List Biological Laboratories (Campbell, USA). HRP goat anti-rabbit antibody (GB23303) was obtained from Servicebio Biotechnology Co., Ltd. Anti-fluorescence quenching solution (P0126) and haematoxylin

Immunogenicity Studies of PA83 variants in Mice

Immunogenicity studies were performed with 3 and 10 μg of plant-produced dPA83(E), dPA83(P) and commercial recombinant PA (rPA, List Biological Laboratories, Campbell, CA, used as a positive control) proteins, administered intraperitoneally in seven- to eight-week-old Balb/c mice (6 animals/group). Mice were injected with three doses of protein samples adsorbed in 0.3% Alhydrogel at three-week intervals (day 0, 21 and 42). …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Goat anti-CTb (1:5000) List Biological Labs Cat# 703; …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Corynebacterium diphtheriae Diphtheria toxoid DipTox Com. product 68,71,72 73,74,75 60 5 Native, List Biological Labs, Campbell, CA …

• Product #151 – Diphtheria Toxoid, from Corynebacterium diphtheriae

… 100 µl cholera toxin (List Biological Laboratories, 100B; …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Systemic LPS challenge

After participants had undergone the IMQ skin challenge, an i.v. LPS challenge was performed on Day 7 after the start of treatment. All participants received 1 ng/kg purified Escherichia coli O113 LPS (List Labs, US, Lot #94332B4) reconstituted in 0.5 mL glucose 2.5%–sodium chloride (NaCl) 0.45% and administered as a 2- min infusion. To ensure that participants stayed adequately hydrated, glucose 2.5%–NaCl 0.45% was infused, starting 2 h prior to LPS administration and continuing until 6 h afterwards.18 Circulating inflammatory proteins (acute phase proteins, cytokines), leukocyte differentiation, and clinical parameters (pulse rate, blood pressure, and temperature) were evaluated over time, starting 0.5 h after the end of the infusion and ending up to 24 h after infusion.

• Product #9433 – GMP LPS Lipopolysaccharide List™ Hpt™ From Escherichia Coli Type O113

… Anthrax lethal toxin List Biological Laboratories Inc. #171B/#172B …

Treatments

See Table S1 for list of Figures/panels in each figure and corresponding dose and exposure time. General information for the methods is provided here. To induce cytokine release from macrophages, microglia and BMDM cells were primed for 4–5 h (or time indicated in Table S1)with 1 μg/mL of LPS in culture medium, and some cells were pre-treated with 1 μM of CP456773/MCC950 for 30 min before ATP stimulation. Cells were then pulsed with 5 mM ATP for 30 min. For cytokine induction by anthrax lethal toxin (LeTx), LPS-primed cells were treated with LeTx at a final concentration of 250 ng/mL (LF and PA) for 3 h. Cell supernatants were collected and frozen at −80°C until assayed.

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis

… Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free) List Biological Cat#181 …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

EAE

EAE was induced as previously described52. In brief, 8–12-week-old female C57Bl/6J mice were immunized using 150 μg of MOG35–55 (Genemed Synthesis, 110582) mixed with freshly prepared Complete Freund’s Adjuvant (using 20 ml of Incomplete Freund’s Adjuvant (BD Biosciences, no. BD263910) mixed with 100 mg of Myobacterium tuberculosis H37Ra (BD Biosciences, no. 231141)) at a ratio of 1:1 (v/v at a concentration of 5 mg ml−1). All mice received two subcutaneous injections of 100 μl each of the MOG35-55/CFA mix. All mice then received a single intraperitoneal injection of 200 ng of pertussis toxin (List Biological Laboratories, no. 180) at a concentration in 200 μl of PBS. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer