Bacterial Toxin Research Citations

EAE:

… Briefly, mice were immunized with 200 µg MOG 3555 peptide…followed by ip injection of 200 ng pertussis toxin (List Biological Laboratories, Campbell, California) on days 0 and 2. EAE development …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

Mice were also injected intraperitoneal (ip) on the same day with 300 ng Pertussis toxin (PT; List Biological Labs, Campbell, California) in 200 µL phosphate buffered saline (PBS) with a booster PT injection 48 hours later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and treatment:

EAE was induced by immunization female mice with myelin oligodendrocyte glycoprotein peptide (MOG _35-55 ) emulsified in complete Freunds adjuvant (Difco Laboratories) at a dose of 100 µg (F1 mice and C57BL/6 J) or 150 µg (NOD mice) per mouse, followed by the administration of pertussis toxin (150 ng per mouse; List biological laboratories, Inc.) on Days 0 and 2 as described.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents and Cell Culture:

… The following probe/antibodies were used: biotin-conjugated cholera toxin B subunit (List Biological Laboratories, Campbell, CA, USA); …

• Product #112 – Cholera Toxin B Subunit Biotin Conjugate

… Injection placements of 3 % Fluoro-Gold (FG; Fluorochrome, LLC, Denver, CO, USA) dissolved in 0.9 % saline, and 2% Cholera Toxin B Subunit (CTB; 104; List Biological Laboratories, Inc., Campbell, CA, USA) dissolved in deionized water were counterbalanced for location …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

EAE induction:

EAE was induced as previously described… Two hundred nanograms of Pertussis toxin (List Biological Laboratories) was administered intraperitoneally (i.p.) to the mice on the immunization day as well as 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Depletion of regulatory T cells in DEREG mice:

For conditional ablation of Tregs, DEREG mice were injected intraperitoneally [ip] twice with 1 g of diphtheria toxin [DT] [List Biological Laboratories, Campbell, CA, USA] 1 day before and on the first day of DSS treatment. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

RNA interference in RAW264.7 cells:

… Transfections were carried out in 96-well format using 200,000 cells/well and 2 m siRNA. Thirty-six hours after siRNA transfection, LTA was added to a final concentration of 2.5 g/ml LTA. Supernatant was collected 5 hr post-LTA treatment, and cytokine production was assayed using DuoSet ELISA kits (R&D Systems). In experiments monitoring the response to lipopolysaccharide (LPS) instead of LTA, LPS was added at a final concentration of 20 ng/ml (LPS from List Biological Laboratories, Campbell, CA).

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Cell culture:

… On the next day, the adherent macrophages were incubated in serum-free medium RPMI1640 at 37C for 2h and then stimulated with LPS (List Biological Laboratories, Escherichia coli 055:B5), …

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

…cholera toxin (List Biological Laboratories, Campbell, CA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae