Bacterial Toxin Research Citations

Electrolyte Solutions and Analytes:

… Anthrax toxins PA63 and LF were obtained from List Biological Labs, Inc. (Campbell, CA, USA). …

Discussion:

We also used EIS to estimate the biochip receiver operating characteristic for the detection of B. anthracis LF. For example, we determined the sensitivity of the PA63 chip to LF (<50 pM), and the response time of the system (minutes). … Antibody 2C11 does not appear to block LF binding to the membrane, which is consistent within vivo measurements of LF activity [76].

… In particular, we demonstrate that AB toxins such as the LF:PA63 from B. anthracis [40] provide a highly specific, built-in sensing scheme.

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

… Emulsions were prepared using an Omni-Mixer (Omni International, Warrenton, VA). Mice also received 300 ng pertussis toxin (List Biological Laboratories, Campbell, CA) intravenously at the time of immunization and 72 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… All mice received 200 ng of pertussis toxin (List Biological Laboratories, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Antibodies and dyes:

…PTX was obtained from List Biological Laboratories (Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…8-12 week-old mice were immunized subcutaneously at the base of the tail with 100 µg of indicated peptides dissolved in 100 µL of PBS, emulsified in 100 µL CFA (Difco) supplemented with 500 µg M. tuberculosis H37RA (Difco). For active EAE, pertussis toxin (List Biological Laboratories, Campbell, CA) was injected i.v. at days 0 and 2 (400 and 200 ng respectively). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… At 0 h and 48 h after immunization, mice were intraperitoneally injected with 200 ng of pertussis toxin (List Biological Laboratories, Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Animals:

Female Foxd1^+/Cre (20) mice were bred with Gt(ROSA)26Sor^{tm1(HBEGF)Awai} mice to generate mice expressing the simian DT receptor (DTR) in FoxD1⁺ progenitor cells and their descendants (Foxd1Cre::RsDTR) in development and littermate controls (RsDTR). In some experiments, female Foxd1^+/Cre mice were crossed with Rosa26^{+/(STOP)-tdTomato} [B6;129S6-Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}] mice. To ablate pericytes, 25 ng/g DT (List Biological Laboratories) was injected intraperitoneally into Foxd1Cre::RsDTR mice or littermate controls. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Tolerance Induction and Food Allergy Model

Tolerance was induced by intragastric administration of 1 mg peanut extract (PE) daily for 5 consecutive days. The murine model of food allergy has been previously described (Li et al., 2000); briefly, mice were sensitized by administration of 1 mg PE with 10 μg of cholera toxin (List Biologicals) in 200 μl of PBS intragastrically on days 0 and 7, followed by a booster challenge with 10 mg PE on day 21. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE induction:

DAPK[CAM]-transgenic, DAPK-knockout, and their WT littermate control mice were immunized subcutaneously with 200µg of MOG peptide 3555 … followed by intraperitoneal injection of 200ng pertussis toxin (List Labs, Campbell, CA, USA) at day 0 and day 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… For treatments, the LSM was amended with 2 g/ml protective antigen (PA) or 2 g/ml lethal toxin (LT, 2 g/ml PA plus 2 g/ml LF, List Biological Laboratories). …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis