Bacterial Toxin Research Citations

November 2, 2024

Inflammation

Karima, S;Khatami, SH;Ehtiati, S;Khoshtinatnikkhouy, S;Kachouei, RA;Jahan-Abad, AJ;Tafakhori, A;Firoozpour, H;Salmani, F;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

heat-killed mycobacterium tuberculosis. Then, this emulsion was injected subcutaneously at the base of the tail of each mouse. The mice also received i.p. injection of pertussis toxin (300 ng in 100 ?l PBS; List Biological Lab, USA) [28, 29]. EAE disease was graded on a 0-5 scale

November 1, 2024

Iscience

Iyer, A;Vaasjo, L;Siththanandan, V;C, R;Thurmon, A;Akumuo, M;Lu, V;Nnebe, C;Nair, R;Galazo, M;Tharin, S;

Product: Cholera Toxin B Subunit (CTB) from Vibrio cholerae FITC, Conjugate

Briefly, corticospinal projection neurons were 585 retrogradely labeled at P1 from the cerebral peduncle by injection of cholera toxin B subunit (CTB) 586 from Vibrio cholerae with FITC conjugate (List Biological Laboratories, Campbell, CA) under 587 ultrasound microscopic guidance. At P2, neuron bodies in the motor cortex were isolated by 588 microdissection of deep cortical layers, and dissoci

November 1, 2024

Jacc: Basic To Translational Science

Zhang, D;Wang, X;Zhu, L;Chen, Y;Yang, C;Zhong, Z;Kong, X;Nan, J;Wang, C;Hu, H;Chen, J;Shi, P;Hu, X;Zhu, W;Wang, J;

Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

e were anesthetized by isoflurane, and mixed AAV solutions (3.2 × 1011 and 2.4 × 1011 vector genomes per mouse, respectively) were injected directly into the myocardium of the left ventricle and apex region. The chest was closed, and the mice were extubated by spontaneous breathing and observed until they were fully awake. To reduce DP macrophages, diphtheria toxin (DT) (List Biological Laboratories) was diluted in PBS and administered to mice IP acutely (25 ng/mg body weight) or chronically (25 ng/mg every other day for 4 weeks). Single-cell RNA sequencing Cell sorting was performed using Aria instrumentation (BD Bioscience). Live single immune cells (4?,6-diamidino-2-phenylindole-CD45+) w

November 1, 2024

Nature Protocols

Tian, S;Qin, Y;Wu, Y;Dong, M;

Product: Anti-Clostridium difficile Toxin B (Chicken lgY)

Mouse monoclonal anti-Actin (Aves Labs, ACT-1010, RRID: AB_2313504) Chicken polyclonal anti-TcdB antibody (List Labs, 754A, RRID: AB_10013220) Chymotrypsin (MedChemExpress, cat. no. HY-108910)

November 1, 2024

Nature Communications

Yang, T;Li, T;Xing, Y;Cao, M;Zhang, M;Leng, Q;Qiu, J;Song, X;Chen, J;Hu, G;Qian, Y;

Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

VA/CFA). For the OVA/cholera toxin allergy model, mice were given drinking water with 0.1% OVA for three days. One week later, 1?mg OVA?+?20??g cholera toxin (100B; List Biological) in 0.2?M sodium bicarbonate was administered once per week for three weeks, with endpoint exa

October 31, 2024

The Journal Of Clinical Investigation

Jiang, Z;Wang, H;Wang, X;Duo, H;Tao, Y;Li, J;Li, X;Liu, J;Ni, J;Wu, EJ;Xiang, H;Guan, C;Wang, X;Zhang, K;Zhang, P;Hou, Z;Liu, Y;Wang, Z;Su, B;Li, B;Hao, Y;Li, B;Wu, X;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Induction and evaluation of EAE

To induce acute EAE, 10 to 12-week-old mice were injected subcutaneously in both flanks of back regions with 300 μg per mice of MOG35-55 peptide (Met-Glu-Val-GlyTrp-Tyr-Arg-Ser-Pro-Phe-Ser-Arg-Val-Val-HisLeu-Tyr-Arg-Asn-Gly-Lys, Genemed Synthesis) in CFA (Incomplete Freund’s adjuvant was purchased from IFA, Sigma) containing 5 mg/ml of heat-killed Mycobacterium tuberculosis H37Ra (BD Difco). Pertussis toxin (200 ng per mouse; List Biological Laboratories) was also administered to mice in PBS on the day of immunization and 48 hours later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

October 30, 2024

Nature

Kim, MW;Gao, W;Lichti, CF;Gu, X;Dykstra, T;Cao, J;Smirnov, I;Boskovic, P;Kleverov, D;Salvador, AFM;Drieu, A;Kim, K;Blackburn, S;Crewe, C;Artyomov, MN;Unanue, ER;Kipnis, J;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

the sequence itself is conserved and corresponds to MBP27-42 of the classic MBP isoform. Pertussis toxin (200?ng, List Biologicals) was injected i.p. on day 0 and day 2 after immunization with MOG and/or

October 30, 2024

Nature Communications

Li, WP;Mao, XT;Xie, JH;Li, JY;Liu, BQ;Wu, LX;Yang, B;Li, YY;Jin, J;

Product: Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

EAE induction

NAT10WT and NAT10cKO mice were anesthetized with pentobarbital and then immunized s.c. with MOG35-55 peptide (2568, R&D; 300 μg) mixed in CFA containing 5 mg/ml heat-killed Mycobacterium tuberculosis H37Ra (231141, Difco). 200 ng pertussis toxin (181, list labs) was i.p. injected to immunized mice subsequently and 24 h later. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

October 29, 2024

Biorxiv

Jank, L;Singh, S;Lee, J;Dhukhwa, A;Siavoshi, F;Joshi, D;Minney, V;Gupta, K;Ghimire, S;Deme, P;Schoeps, V;Soman, K;Ladakis, D;Smith, M;Borkowski, K;Newman, J;Baranzini, S;Waubant, E;Fitzgerald, K;Mangalam, A;Haughey, N;Kornberg, M;Chamling, X;Calabresi, P;Bhargava, P;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

EAE induction and ILA supplementation

Experimental autoimmune encephalomyelitis (EAE) was induced in 10–12-week-old C57BL/6J mice through subcutaneous injection of 150 µg of MOG35-55 peptide (JHMI Peptide Synthesis Core, USA), emulsified in Complete Freund’s Adjuvant (CFA; Thermo Scientific, USA) containing 8 mg/ml of heat-killed Mycobacterium tuberculosis (BD, NJ). The injection was administered at two sites on the abdomen. To further enhance the immune response, 300 ng of pertussis toxin (List Biological Labs, California, USA) was administered intraperitoneally on the day of immunization, followed by a second dose 48 hours later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

October 29, 2024

Available At SSRN

Randhawa, G;Mobarakabadi, M;DMello, C;Morch, M;Zhang, P;Ling, C;Yong, V;GHORBANI, S;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

EAE:
Female WT and Fbln2-/- mice (10–12 weeks old) were injected subcutaneously with 50 μg MOG35–55 peptide (synthesized by Protein and Nucleic acid Facility, Stanford University, Stanford, California, USA) emulsified in complete Freund’s adjuvant (CFA) (Thermo Fisher Scientific) supplemented with 10 mg/mL heat-inactivated Mycobacterium tuberculosis H37Ra (MilliporeSigma). A total of 100 μL emulsion was injected at 2 sites into hind flanks. On the day of immunization and 48 hours later, Pertussis toxin (300 ng per 200 μL; List Biological Laboratories) was injected intraperitoneally. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Bacterial Toxin Research Citations

Acetyl 11-Keto Beta-Boswellic Acid Improves Neurological Functions in a Mouse Model of Multiple Sclerosis

MiR-193b-365 microcluster downstream of Fezf2 coordinates neuron-subtype identity and dendritic morphology in cortical projection neurons

TIMD4hiMHC?hi Macrophages Preserve Heart Function Through Retnla

Design, performance, processing, and validation of a pooled CRISPR perturbation screen for bacterial toxins

Dietary nucleic acids promote oral tolerance through innate sensing pathways in mice

TMED4 facilitates Treg suppressive function via ROS homeostasis in tumor and autoimmune mouse models

Endogenous self-peptides guard immune privilege of the central nervous system

N-acetyltransferase 10 is implicated in the pathogenesis of cycling T cell-mediated autoimmune and inflammatory disorders in mice

Restoring the Multiple Sclerosis Associated Imbalance of Gut Indole Metabolites Promotes Remyelination and Suppresses Neuroinflammation

Targeting Extracellular Matrix Components to Attenuate Microglia Neuroinflammation: A Study of Fibulin-2 and Cspgs in a Model of Multiple Sclerosis