Citations

… Ultra Pure LPS (Escherichia coli 0111:B4, LIST Biological Laboratories, Campbell, Calif., USA) was dissolved in sterile normal saline and injected intraperitoneally (1 mg/kg) every 2 days to induce chronic endotoxemia (n = 9 for each designated time point). …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Murine EAE model:

EAE was induced in female WT and Abcc8/ mice at 8 weeks of age, …Each mouse then received 400 ng of pertussis toxin (List Biological Laboratories) intraperitoneally (IP) on post-induction day (pid) 0 and pid-2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… LPS was obtained from List Biological Laboratories, Inc (Campbell, CA). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Immunizations and Infections:

For Treg ablation experiments, Foxp3-DEREG mice were injected intraperitoneally with 75 g/kg diphtheria toxin (List Biologicals) at days 1 and 2 post antigen immunization.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Reagent:  Cholera toxin B subunit (CTB) was from List Biological Laboratories.

Macrophage Culture, Transfection, Infection, and Cytotoxicity Assay:

For CTB treatment, primed cells were stimulated with 20 µg/ml CTB plus 1 µg/ml LPS (Kayagaki et al., 2011 and 2013).

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Cell culture and stimulation:

…For stimulation, the medium was exchanged for 2h and supplemented with 101000ng/ml LPS (ultrapure smooth LPS from E. coli O111:B4, List Biological Laboratories, Campbell, CA). …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

… Bordetella pertussis toxin (200 ng, PTX, List Biological Laboratories, Campbell, CA) was administered intraperitoneally at day 0 and 2 post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… by subcutaneous injection of 50 µg of MOG 3555 in Freund’s Complete Adjuvant (Thermo Fisher Scientific, Rockford, USA) supplemented with 4 mg/ml of Mycobacterium tuberculosis on day 0. Intraperitoneal pertussis toxin (0.1 µg/200 µl; List Biological Laboratories, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…containing 500 µg of Mycobacterium tuberculosis H37RA (Difco, Detroit, USA). Immediately after immunization and again 48 h later, the mice received a tail vein injection of 200 ng of Pertussis toxin (List Biological Laboratories, CA, USA) in 100 µl of PBS. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and KYC treatment:

For MOG-induced EAE, mice on the C57BL/6 background were subcutaneously immunized with 200 µg MOG₃₅₅₅ peptide emulsified in an equal volume of complete Freund’s adjuvant…containing 4 mg/mL mycobacterium followed by i.p. injection of 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA, USA) on days 0 and 2, as described…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)