Bacterial Toxin Research Citations

Anterograde Labeling of Vagal Afferents:

Each rat was given atropine (0.1 mg/ml, s.c.) 15 minutes prior to surgery to prevent airway secretions during surgery, … and CTb (1 to 2 l of 1-2% solution in deionized water, List Biological Laboratories) were pressure injected slowly into the nerve with a single-barrel glass micropipette, …

Perfusion and Immunocytochemistry:

… To examine channel content within CTb-labeled vagal afferents, alternating sections were incubated in the same cocktail with the exception of a polyclonal goat antibody directed against cholera toxin subunit B (1:25000; List Biological Laboratories, Cat: 703) instead of the IB4 antibody. …

Author did not specify which CTB was utilized.  List Labs provides Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae)  and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Histological assessment of contusion injury and phrenic motoneuron number:

…Spinal cord sections were systematically and randomly immunostained (every fourth section). Goat immunoglobulin G (IgG) anti-CTB (List Biological Laboratories Inc., Campbell, CA) and…were used as primary and secondary antibodies, respectively. The specificity of these antibodies has been validated previously.

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Tolerance conditions:

To determine the effect of MPLA and LPS on inducing tolerance in HUVECs, cells were exposed combinations of…100 ng/ml of Ultra-Pure LPS (E. coli 0111:B4, List Biological Laboratories, Inc. Campbell, CA), or control (2% FBS media) for 16 hours in media containing 2% FBS (1^st treatment)…Afterwards, cells were challenged with of LPS (100 ng/ml) in 2% FBS media (2^nd treatment). LPS derived from E. coli was chosen based on previous experiments examining the inhibitory kinetics of MPLA [17], in addition to the more common use of E. coli LPS in models of endotoxic shock…

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Reagents…for stimulation of human monocytes, LPS (O111:B4) was from List Biologicals.

• Product #201 – LPS from Escherichia coli O111:B4

Ca²⁺ current recording:

In some experiments, CSMG neurons were pretreated overnight with either …or 0.5 µg/µl PTX (List Biological Laboratories, Campbell, CA) in the culture medium. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAU:

We induced EAU in female B10.A mice, aged 6 to 10 weeks, by immunization with IRBP as described elsewhere,^29,30 with minor modifications. The mice were immunized with 40 µg bovine IRBP emulsified with complete Freund’s adjuvant (CFA) and injected subcutaneously into the base of the tail and both thighs. In addition, the mice were injected intraperitoneally with 0.2 µg pertussis toxin (List Laboratories, Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Complete Freund’s adjuvant was obtained from Sigma-Aldrich (St Louis, MO, USA). Heat-killed M. tuberculosis strain H37Ra was obtained from Difco (Detroit, MI, USA), and pertussis toxin was obtained from List Biological Laboratories (Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAM:

… Mice were also injected with 500 ng of pertussis toxin ip on day 0 (List Biologicals). Induction of EAM by Adoptive Transfer of CD4+ T Cells …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

Experimental autoimmune encephalomyelitis was induced as previously described… Bordetella pertussis Brain Struct Funct 123 toxin (250 ng; List Biological Laboratories, USA) was administered intraperitoneally on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.3. Induction of RR-EAE and in vivo treatments:

… Each mouse received s.c. injections of 200 µl emulsion, fractionated in two distinct sites draining axillary and inguinal lymph nodes. Pertussis toxin (PTX, 200 ng/mouse; List Biological Laboratories) was administered i.p. on the day of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)