Citations

Induction of EAE:

For EAE induction, mice were immunized s.c. over two sites on the lateral abdomen with 100 mg MOG 3555 peptide…followed by i.p. injections of 250 ng Bordetella pertussis toxin (List Biological Laboratories, Campbell, CA) on days 0 and 2 after immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

C57BL/6 mice were immunized s.c. with 200 mg myelin oligodendrocyte glycoprotein (MOG)3555 peptide…followed by iv injection with 200 ng pertussis toxin (List Biological Laboratories) on…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Three of them were given a unilateral eye injection (left eye) of CTB (List Biologicals, Campbell, CA). The eye was prepunctured at a dorsotemporal location, and a nominal 1 µL of a 1% solution of the toxin in water was delivered through a glass semimicropipet. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Reagents and their sources were as follows:

Escherichia coli LPS serotype O1101:B4 (List Biological Laboratories);…

Isolation, Culture, and Experimental Testing of BMDC:

… BMDC were routinely primed with 100 ng/ml LPS for 4 h to activate TLR4 signaling prior to treatment with 10 m nigericin (30 min to 6 h) or 240480 g/ml alum (6 h). …

• Product #201 – LPS from Escherichia coli O111:B4

List Biological Laboratories INC. Certificate of Analysis-Tetanus Toxiod. …

• Product #191 – Tetanus Toxoid from Clostridium tetani

… Pertussis toxin (200 ng, List Biological Laboratories, US) was administered intraperitoneal on days 0 and 2. Mice were weighed and scored daily as …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cholera Toxin Subunit B (CTB):

The polyclonal goat anti-choleragenoid antibody (703, LOT 10327A4A, List Laboratories Inc., Campbell, CA, USA) was used to detect the tracer CTB (103B, List) provided by the same manufacturer. This tracing and detection method has been successfully applied in numerous previous studies…

• Product #103B – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae
• Product # 703 – Anti-Cholera Toxin B Subunit (Goat)

Drugs:

…Pertussis toxin was from List biological laboratories Inc. (Campbell, CA 95008, US). …

Results – Effect of Pertussis-toxin treatment:

In order to elucidate the identity of the endogenous G subunits mediating the NOP/G₁ interaction, and to evaluate their potential effects on NOP mediated -arrestin 2 recruitment, HEK293 cells stably expressing the human NOP (NOP/RLuc) receptor and either the G₁ subunit (G₁/RGFP) or the -arrestin 2 (-arrestin 2/RGFP) were treated for 20 h with 10 ng PTX. As shown in Fig 2, right panel, PTX treatment abolished the ability of the agonist to stimulate NOP/G-protein interaction, but had negligible effects on the stimulation of NOP/-arrestin 2 interactions…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell culture, transfection and selection

The Breast cancer cell lines MDA-MB-231 and MCF-7 (ATCC) were maintained in DMEM (Sigma) supplemented with fetal bovine serum (FBS, Invitrogen) and antibiotics and antimycotics (ABM, Invitrogen). Normal breast cell lines: MCF-12A and MCF10A (ATCC) were cultured in universal medium composed of DMEM/F12 medium supplemented with 10 μg/mL insulin, 20 ng/mL epidermal growth factor (EGF), 500 ng/mL hydrocortisone (all from Sigma) and 100 ng/mL cholera Toxin (LIST Biological Laboratories, Surrey, United Kingdom), 5 % horse serum (Invitrogen) and 1 % ABM. All established cell lines were used within 6 months of purchase from ATCC. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Cell culture:

Keratinocyte culture medium (KCM) was composed of a basal mixture of 3 parts DMEM to 1 part nutrient mixture F-12 Ham ), …1 nM cholera toxin (List Biological Labs, Campbell, CA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae